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Three-dimensional structure of a complex of antibody with influenza virus neuraminidase

Nature volume 326, pages 358–363 (1987)Cite this article

Abstract

The structure of a complex between influenza virus neuraminidase and an antibody displays features inconsistent with the inflexible 'lock and key' model of antigen‐antibody binding. The structure of the antigen changes on binding, and that of the antibody may also change; the interaction therefore has some of the character of a handshake.

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References

  1. Colman, P. M. & Ward, C. W. Curr. Top. Microbiol. Immun. 114, 178–255 (1985).

    Google Scholar 

  2. Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. Sequences of Proteins of Immunological Interest (US Department of Health and Public Services, Washington DC, 1983).

    Google Scholar 

  3. Schiffer, M., Girling, R. L., Ely, K. R. & Edmundson, A. B. Biochemistry 12, 4620–4631 (1973).

    Article  CAS  PubMed  Google Scholar 

  4. Poljak, R. J. et al. Proc. natn. Acad. Sci. U.S.A. 70, 3305–3310 (1973).

    Article  ADS  CAS  Google Scholar 

  5. Segal, D. M. et al. Proc. natn. Acad. Sci. U.S.A. 71, 4298–4302 (1974).

    Article  ADS  CAS  Google Scholar 

  6. Matsushima, M. et al. J. molec. Biol. 121, 441–459 (1978).

    Article  CAS  PubMed  Google Scholar 

  7. Saul, F., Amzel, L. M. & Poljak, R. J. J. biol. Chem. 253, 585–597 (1978).

    CAS  PubMed  Google Scholar 

  8. Marquart, M., Deisenhofer, J., Huber, R. & Palm, W. J. molec. Biol. 141, 369–392 (1980).

    Article  CAS  PubMed  Google Scholar 

  9. Satow, Y., Cohen, O. H., Padlan, E. A. & Davies, D. R. J. molec. Biol. 190, 593–604 (1986).

    Article  CAS  PubMed  Google Scholar 

  10. Suh, S. W. et al. Proteins: Structure, Function and Genetics, 1, 74–80 (1986).

    Article  CAS  Google Scholar 

  11. Colman, P. M. & Webster, R. G. in Biological Organisation: Macromolecular Interactions at High Resolution (ed. Burnett, R.) 125–133 (Academic, New York, 1987).

    Google Scholar 

  12. Amit, A. G., Marriuzza, R. A., Phillips, S. E. V. & Poljak, R. J. Science 233, 747–753 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Novotny, J. & Haber, E. Proc. natn. Acad. Sci. U.S.A. 82, 4592–4596 (1985).

    Article  ADS  CAS  Google Scholar 

  14. Davies, D. R., Padlan, E. A. & Segal, D. M. A. Rev. Biochem. 44, 639–667 (1975).

    Article  CAS  Google Scholar 

  15. Chothia, C., Novotny, J., Bruccoleri, R. & Karplus, M. J. molec. Biol. 186, 651–663 (1986).

    Article  Google Scholar 

  16. Padlan, E. A., Cohen, G. H. & Davies, D. R. Molec. Immun. 23, 951–960 (1986).

    Article  CAS  PubMed  Google Scholar 

  17. Colman, P. M., Deisenhofer, J., Huber, R. & Palm, W. J. molec. Biol. 100, 257–282 (1976).

    Article  CAS  PubMed  Google Scholar 

  18. Davies, D. R. & Metzger, H. A. Rev. Immun. 1, 87–117 (1983).

    Article  CAS  Google Scholar 

  19. Benjamin, D. C. et al. A. Rev. Immun. 2, 67–101 (1984).

    Article  CAS  Google Scholar 

  20. Crumpton, M. J. & Wilkinson, J. M. Biochem. J. 94, 545–556 (1965).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Smith-Gill, S. J. et al. J. Immun. 128, 314–322 (1982).

    CAS  PubMed  Google Scholar 

  22. Lerner, R. A. Nature 299, 592–596 (1982).

    Article  ADS  CAS  Google Scholar 

  23. Tainer, J. A. et al. Nature 312, 127–134 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Westhof, E. et al. Nature 311, 123–126 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Metzger, H. Contemp. Top. molec. Immun. 7, 119–152 (1978).

    Article  CAS  Google Scholar 

  26. Laver, W. G. Virology 86, 78–87 (1978).

    Article  CAS  PubMed  Google Scholar 

  27. Schulman, J. L. in The Influenza Viruses and Influenza (ed. Kilbourne, E. D.) 373–393 (Academic, New York, 1975).

    Google Scholar 

  28. Webster, R. G., Laver, W. G. & Air, G. M. in Genetics of Influenza Viruses (ed. Palese, P. & Kingsbury, B. W.) 127–168 (Springer, New York, 1983).

    Book  Google Scholar 

  29. Varghese, J. N., Laver, W. G. & Colman, P. M. Nature 303, 35–40 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Colman, P. M., Varghese, J. N. & Laver, W. G. Nature 303, 41–44 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  31. Laver, W. G., Colman, P. M., Webster, R. G., Hinshaw, V. S. & Air, G. M. Virology 137, 314–323 (1984).

    Article  CAS  PubMed  Google Scholar 

  32. Air, G. M., Ritchie, L. R., Laver, W. G. & Colman, P. M. Virology 145, 117–122 (1985).

    Article  CAS  PubMed  Google Scholar 

  33. Tulloch, P. A. et al. J. molec. Biol. 190, 215–225 (1986).

    Article  CAS  PubMed  Google Scholar 

  34. Laver, W. G., Webster, R. G. & Colman, P. M. Virology 156, 181–184 (1987).

    Article  CAS  PubMed  Google Scholar 

  35. Epp, O. et al. Biochemistry 14, 4943–4952 (1975).

    Article  CAS  PubMed  Google Scholar 

  36. Epp, O. et al. Eur. J. Biochem. 45, 513–524 (1974).

    Article  CAS  PubMed  Google Scholar 

  37. Colman, P. M., Schramm, H. J. & Guss, J. M. J. molec. Biol. 116, 73–79 (1977).

    Article  CAS  PubMed  Google Scholar 

  38. Furey, W., Wang, B. C., Yoo, C. S. & Sax, M. J. molec. Biol. 167, 661–692 (1983).

    Article  CAS  PubMed  Google Scholar 

  39. Chang, C. H. et al. Biochemistry 24, 4890–4897 (1985).

    Article  CAS  PubMed  Google Scholar 

  40. Holowka, D. A., Strosberg, A. D., Kimball, J. W., Haber, E. & Cathou, R. E. Proc. natn. Acad. Sci. U.S.A. 69, 3399–3403 (1972).

    Article  ADS  CAS  Google Scholar 

  41. Levison, S. A., Hicks, A. N., Portman, A. J. & Dandliker, W. B. Biochemistry 14, 3778–3786 (1975).

    Article  CAS  PubMed  Google Scholar 

  42. Schlessinger, J., Steinberg, I. Z., Givol, I. D., Hochman, J. & Pecht, I. Proc. natn. Acad. Sci. U.S.A. 72, 2775–2779 (1975).

    Article  ADS  CAS  Google Scholar 

  43. Lancet, D. & Pecht, I. Proc. natn. Acad. Sci. U.S.A. 73, 3549–3553 (1976).

    Article  ADS  CAS  Google Scholar 

  44. Zidovetzki, R., Blatt, Y. & Pecht, I. Biochemistry 20, 5011–5018 (1981).

    Article  CAS  PubMed  Google Scholar 

  45. Edmundson, A. B., Ely, K. R. & Herron, J. N. Molec. Immun. 21, 561–576 (1984).

    Article  CAS  PubMed  Google Scholar 

  46. Ely, K. R. et al. Biochemistry 17, 158–167 (1978).

    Article  CAS  PubMed  Google Scholar 

  47. Jackson, D. C. & Webster, R. G. Virology 123, 69–77 (1982).

    Article  CAS  PubMed  Google Scholar 

  48. Mandel, B., Virology 69, 500–510 (1976).

    Article  CAS  PubMed  Google Scholar 

  49. Lubeck, M. & Gerhard, W. Virology 118, 1–7 (1982).

    Article  CAS  PubMed  Google Scholar 

  50. Crumpton, M. J. Biochem. J. 100, 223–232 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Amon, R. The Antigens Vol. 1 (ed. Sela, M.) 87–159 (Academic, New York, 1973).

    Google Scholar 

  52. Dimmock, N. J. J. gen. Virol 65, 1015–1022 (1984).

    Article  PubMed  Google Scholar 

  53. Srinivasappa, J. et al. J. Virol. 57, 397–401 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  54. Tonegawa, S. Sci. Am. 253, 104–113 (1985).

    Article  Google Scholar 

  55. Barth, R. K. et al. Nature 316, 517–523 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  56. Arden, B., Klotz, J., Siu, G. & Hood, L. E. Nature 316, 783–787 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  57. Goverman, J., Hunkapillar, T. & Hood, L. E. Cell 45, 475–484 (1986).

    Article  CAS  PubMed  Google Scholar 

  58. Amyard-Henry, M. et al. Bull. WHO 48, 199–202 (1973).

    Google Scholar 

  59. Air, G. M. Virology 97, 468–472 (1979).

    Article  CAS  PubMed  Google Scholar 

  60. Wang, B. C. Meth. Enzym. 115, 90–112 (1985).

    Article  CAS  PubMed  Google Scholar 

  61. Sussman, J. L. in Methods and Applications in Crystallographic Computing (eds Hall, S. R. & Ashida, T.) 206–237 (Oxford University Press, 1984).

    Google Scholar 

  62. Hendrickson, W. A. & Konnert, J. H. in Biomolecular Structure, Conformation, Function and Evolution Vol. 1 (ed. Srinivasan, R.) 43–57 (Pergamon, Oxford, 1981).

    Book  Google Scholar 

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Author notes

  1. W. G. Laver: John Curtin School of Medical Research, Australian National University, Canberra 2601, Australia

  2. G. M. Air: Department of Microbiology, University of Alabama, Birmingham, Alabama 35294, USA

  3. R. G. Webster: St Jude Children's Research Hospital, Memphis, Tennessee 38101, USA

Authors and Affiliations

  1. CSIRO Division of Protein Chemistry, 343 Royal Parade, Parkville, 3052, Australia

    P. M. Colman, W. G. Laver, J. N. Varghese, A. T. Baker, P. A. Tulloch, G. M. Air & R. G. Webster

Authors
  1. P. M. Colman
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  2. W. G. Laver
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  3. J. N. Varghese
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  5. P. A. Tulloch
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  6. G. M. Air
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  7. R. G. Webster
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Colman, P., Laver, W., Varghese, J. et al. Three-dimensional structure of a complex of antibody with influenza virus neuraminidase. Nature 326, 358–363 (1987). https://doi.org/10.1038/326358a0

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