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Ion channels activated by inositol 1,4,5-trisphosphate in plasma membrane of human T-lymphocytes

Nature volume 326, pages 301–304 (1987)Cite this article

Abstract

Hydrolysis of membrane-associated phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)-P2) to water soluble inositol 1,4,5-trisphosphate (Ins(l,4,5)P3) is a common response by many different kinds of cells to a wide variety of external stimuli (see refs 1 and 2 for review). Ins (1,4,5)P3 is a putative second messenger which increases intracellular Ca2+ by mobilizing internal Ca2+ stores, a hypothesis which has been substantiated by studies with chemically permeabilized cells3–13 and with isolated microsomal membrane fractions11,12–16. But the possibility that Ins(1,4,5)P3 could induce in intact cells an influx of external Ca2+ through transmembrane channels, originally hypothesized by Michell in 197517, has never been directly tested. We report here single-channel recordings of an Ins(1,4,5)P3-activated conductance in excised patches of T-lymphocyte plasma membrane. The Ins(1,4,5)P3-activated transmembrane channel appears to be identical to the recently described mitogen-regulated, voltage-insensitive Ca2+ permeable channel involved in T-cell activation18. We suggest that Ins(1,4,5)P3 acts as the second messenger mediating transmembrane Ca2+ influx through specific Ca2+-permeable channels in mitogen-stimulated T-cell activation.

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Author notes

  1. Miyuki Kuno

    Present address: Department of Physiology, Osaka City University Medical School, Abenkoku, Osaka, 545, Japan

  2. Phyllis Gardner: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Medicine, Stanford University School of Medicine, Stanford, California, 94305, USA

    Miyuki Kuno & Phyllis Gardner

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  1. Miyuki Kuno
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  2. Phyllis Gardner
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Kuno, M., Gardner, P. Ion channels activated by inositol 1,4,5-trisphosphate in plasma membrane of human T-lymphocytes. Nature 326, 301–304 (1987). https://doi.org/10.1038/326301a0

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