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Differential expression of two distinct T-cell receptors during thymocyte development


The product of the T-cell receptor (TCR) γ-gene1 has recently been found to be expressed on a subset of both peripheral cells2 and thymocytes3,4. As an initial approach to understanding the role of this γ-chain of TCR (TCRγ) in T-cell development, we have studied the ontogeny of TCR expression at the protein level in the developing murine thymus. We show here that the first T3-associated TCR to be expressed in the developing thymus is a disulphide-linked heterodimer composed of a γ-chain of relative molecular mass 35,000 (Mr 35K) and a 45K partner (termed TCR δ). This TCR γ δ is first detected approximately two days before the appearance of cell-surface TCR αβ heterodimers. We report that N-glycosidase digestions reveal that all of the γ-protein expressed on fetal thymocytes, as in adult CD48 (L3T4, Lyt2) thymocytes4, bear N-linked carbohydrate side chains. The major γ-gene transcribed in mature, αβ-bearing T cells ( Vγ1.2 Cγ2) encodes no N-linked glycosylation site1 so these results suggest that the fetal γδ receptor defines a distinct T-cell lineage whose development in the thymus precedes classical αβ -bearing cells.

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  1. Saito, H. et al. Nature 309, 757–762 (1984).

    Article  ADS  CAS  Google Scholar 

  2. Brenner, M. B. et al. Nature 322, 145–149 (1986).

    Article  ADS  CAS  Google Scholar 

  3. Bank, I. et al. Nature 322, 179–181 (1986).

    Article  ADS  CAS  Google Scholar 

  4. Lew, A. M. et al. Science 234, 1401–1405 (1987).

    Article  ADS  Google Scholar 

  5. Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., & Bluestone, J. A. Proc. natn. Acad. Sci. U.S.A. (in the press).

  6. Bluestone, J. A., Pardoll, D. M., Sharrow, S. & Fowlkes, B. J. Nature 326, 82–84 (1987).

    Article  ADS  CAS  Google Scholar 

  7. Ceredig, R., Dialynas, D. P., Fitch, F. W. & MacDonald, H. R. J. exp. Med. 158, 1654–1671 (1983).

    Article  CAS  Google Scholar 

  8. Raulet, D. H., Garman, R. D., Saito, H. & Tonegawa, S. Nature 314, 103–107 (1985).

    Article  ADS  CAS  Google Scholar 

  9. Helig, J. S. & Tonegawa, S. Nature 322, 836–840 (1986).

    Article  ADS  Google Scholar 

  10. Born, W., Rathbun, G., Tucker, P., Marrack, P. & Kappler, J. Science 234, 479–482 (1986).

    Article  ADS  CAS  Google Scholar 

  11. Garman, R. D., Doherty, P. J. & Raulet, D. H. Cell 45, 733–742 (1986).

    Article  CAS  Google Scholar 

  12. Hayday, A. C. et al. Cell 40, 259–269 (1985).

    Article  CAS  Google Scholar 

  13. Snodgrass, H. R., Dembic, Z., Steinmetz, M. & von Boehmer, H. Nature 315, 232–233 (1985).

    Article  ADS  CAS  Google Scholar 

  14. Langman, R. E. Rev. Physiol. Biochem. Pharmac. 81, 1–36 (1978).

    CAS  Google Scholar 

  15. Lew, A. M., Maloy, W. L. & Coligan, J. E. J. lmmun. 136, 254–258 (1986).

    CAS  Google Scholar 

  16. Tarentino, A. L., Gomez, C. M. & Plummer, T. H. Biochemistry 24, 4665–4671 (1985).

    Article  CAS  Google Scholar 

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Pardoll, D., Fowlkes, B., Bluestone, J. et al. Differential expression of two distinct T-cell receptors during thymocyte development. Nature 326, 79–81 (1987).

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