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Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence

Nature volume 325, pages 622–625 (1987)Cite this article

Abstract

Eukaryotic messenger RNAs are translated with unequal efficiencies in vivo1,2 and in vitro3–5 and the molecular basis of this phenomenon is not understood. As an approach to understanding the role of the 5' untranslated leader sequence in regulating mRNA translational efficiency, chimaeric mRNAs have been generated by joining a heterologous leader to complementary DNA (cDNA) sequences, followed by in vitro transcription using SP6 RNA polymerase and in vitro protein synthesis. We used the untranslated leader from the coat protein mRNA of alfalfa mosaic virus (AMVRNA 4), a well-translated, highly competitive message5,6, to replace the leader sequence of barley α-amylase (BαA) and human interleukin 1β (IL-1β)cDNAs. Deletion of transcribed vector sequences and replacement of the native untranslated region with the AMV RNA 4 leader can result in as much as a 35-fold increase in mRNA translational efficiency; moreover, the translational efficiency of the chimaeric mRNAs containing the AMV RNA 4 leader is at least as great as that of virion RNA 4. The results suggest that the chimaeric AMV-mRNAs have either a higher relative affinity or a diminished requirement for a limiting component(s) of the translational machinery; in addition, it may be feasible, through use of heterologous leader sequences, to increase expression of engineered genes or cDNAs without changing the antigenic or biological properties of the encoded protein.

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References

  1. Cordell, B. et al. Cell 31, 531–542 (1982).

    Article  CAS  Google Scholar 

  2. Alton, T. H. & Lodish, H. F. Cell 12, 301–310 (1977).

    Article  CAS  Google Scholar 

  3. Lodish, H. F. A. Rev. Biochem. 45, 39–72 (1976).

    Article  CAS  Google Scholar 

  4. Kabat, D. & Chappel, M. R. J. biol. Chem. 252, 2684–2690 (1977).

    CAS  Google Scholar 

  5. Herson, D., Schmidt, A., Seal, S. N., Marcus, A. & van Vloten Doting, L. J. biol. Chem. 254, 8245–8249 (1979).

    CAS  Google Scholar 

  6. Godefroy-Colbum, T., Thivent, C. & Pinck, L. Eur. J. Biochem. 147, 541–548 (1985).

    Article  Google Scholar 

  7. Gehrke, L., Auron, P. E., Quigley, G. Q., Rich, A. & Sonenberg, N. Biochemistry 22, 5157–5164 (1983).

    Article  CAS  Google Scholar 

  8. Rogers, J. C. & Milliman, C. J. biol. Chem. 258, 8169–8174 (1983).

    CAS  PubMed  Google Scholar 

  9. Auron, P. E. et al. Proc. natn. Acad. Sci. U.S.A 81, 7907–7911 (1984).

    Article  ADS  CAS  Google Scholar 

  10. Kozak, M. Nature 308, 241–246 (1984).

    Article  ADS  CAS  Google Scholar 

  11. Kozak, M. Cell 44, 283–292 (1986).

    Article  CAS  Google Scholar 

  12. Ehrenfeld, E. Cell 28, 435–436 (1982).

    Article  CAS  Google Scholar 

  13. Van Vloten-Doting, L. & Neeleman, L. in Translation of Plant Virus RNAs (eds Parthier, B. & Boulter, D.) 337–367 (Springer, New York, 1982).

    Google Scholar 

  14. Zagorski, W. Eur. J. Biochem. 86, 465–472 (1978).

    Article  CAS  Google Scholar 

  15. Joshi, S. & Haenni, A.-L. FEBS Lett. 177, 163–174 (1984).

    Article  CAS  Google Scholar 

  16. Atabekov, J. G. & Morozov, S. Yu. Adv. Virus Res. 25, 1–91 (1979).

    Article  CAS  Google Scholar 

  17. Loesch-Fries, L. S., Jarvis, N. P., Krahn, K. J., Nelson, S. E. & Hall, T. C. Virology 146, 177–187 (1985).

    Article  CAS  Google Scholar 

  18. Friesen, P. D. & Rueckert, R. R. J. Virol. 49, 116–124 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Gough, N. M., Metcalf, D., Gough, J., Grail, D. & Dunn, A. R. EMBO J. 4, 645–653 (1985).

    Article  CAS  Google Scholar 

  20. Iserentant, D. & Fiers, W. Gene 9, 1–12 (1980).

    Article  CAS  Google Scholar 

  21. Sonenberg, N., Guertin, D., Cleveland, D. & Trachsel, H. Cell 27, 563–572 (1981).

    Article  CAS  Google Scholar 

  22. McGarry, T. J. & Lindquist, S. Cell 42, 903–911 (1985).

    Article  CAS  Google Scholar 

  23. Ray, B. K. et al. J. biol. Chem. 260, 7651–7658 (1985).

    CAS  PubMed  Google Scholar 

  24. Sonenberg, N., Guertin, D. & Lee, K. A. W. Molec. cell Biol. 2, 1633–1638 (1982).

    Article  CAS  Google Scholar 

  25. Gehrke, L. Gene Analysis Techniques 3, 45–52 (1986).

    Article  CAS  Google Scholar 

  26. Brederode, R. T., Koper-Zwarthoff, E. C. & Bol, J. F. Nucleic Acids Res. 8, 2213–2223 (1980).

    Article  CAS  Google Scholar 

  27. Kozak, M. Microbiol. Rev. 47, 1–45 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Kozak, M. Nature 308, 241–246 (1984).

    Article  ADS  CAS  Google Scholar 

  29. Lockard, R. E. & RajBhandary, U. L. Cell 9, 747–760 (1976).

    Article  CAS  Google Scholar 

  30. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning; a Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  31. Edery, I. & Sonenberg, N. Proc. natn. Acad. Sci. U.S.A. 82, 7590–7594 (1985).

    Article  ADS  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, E25–545, Cambridge, Massachusetts, 02139, USA

    Stephen A. Jobling & Lee Gehrke

  2. Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Lee Gehrke

Authors
  1. Stephen A. Jobling
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  2. Lee Gehrke
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Jobling, S., Gehrke, L. Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence. Nature 325, 622–625 (1987). https://doi.org/10.1038/325622a0

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