Abstract
Cytosolic dihydrofolate reductase from mouse contains a cryptic mitochondrial targeting sequence. If this sequence is attached to the amino terminus of 'passenger' proteins which by themselves cannot enter mitochondria, the resulting fusion proteins are transported into yeast mitochondria.
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References
Hurt, E. C. & van Loon, A. P. G. M. Trends Biochem. Sci. 11, 204–207 (1986).
Eilers, M. & Schatz, G. Nature 322, 228–232 (1986).
Roise, D., Horvath, S. J., Tomich, J. M., Richards, J. H. & Schatz, G. EMBO J. 5, 1327–1334 (1986).
von Heijne, G. EMBO J. 5, 1335–1342 (1986).
Allison, D. & Schatz, G. Proc. natn. Acad. Sci. U.S.A. 83, 9011–9015 (1986).
Volz, K. W. et al. J. biol. Chem. 257, 2528–2536 (1982).
Hurt, E. C., Pesold-Hurt, B. & Schatz, G. EMBO J. 3, 3149–3156 (1984).
Hurt, E. C., Müller, U. & Schatz, G. EMBO J. 4, 3509–3518 (1985).
Hurt, E. C., Pesold-Hurt, B., Suda, K., Oppliger, W. & Schatz, G. EMBO J. 4, 2061–2068 (1985).
Horwich, A. L., Kalousek, F. Fento, W. A., Pollock, R. A. & Rosenberg, L. E. Cell 44, 451–459 (1986).
Dowhan, W., Bibus, C. R. & Schatz, G. EMBO J. 4, 179–184 (1985).
Kabsch, W. & Sander, C. Proc. natn. Acad. Sci. U.S.A. 81, 1075–1078 (1984).
Baker, A. & Schatz, G. Proc. natn. Acad. Sci. U.S.A. (in the press).
Stone, D. & Philips, A. W. FEBS Lett. 74, 85–87 (1986).
Suissa, M. Analyt. Biochem. 133, 511–514 (1983).
Stueber, D., Ibrahimi, I., Cutler, D., Dobberstein, B. & Bujard, H. EMBO J. 3, 3143–3148 (1984).
Hurt, E. C., Pesold-Hurt, B. & Schatz, G. FEBS Lett. 178, 306–310 (1984).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
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Hurt, E., Schatz, G. A cytosolic protein contains a cryptic mitochondrial targeting signal. Nature 325, 499–503 (1987). https://doi.org/10.1038/325499a0
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DOI: https://doi.org/10.1038/325499a0
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