Abstract
L3T4, Lyt-2 and the T-cell receptor for antigen are cell-surface molecules involved in antigen specific T cell activation. We have constructed functional murine cytolytic-helper T-cell hybrid clones to study the link between expression of cell-surf ace molecules and specific cell function. Three of the clones express two antigen receptors; and both Lyt-2 and L3T4, normally expressed on mutually exclusive subsets of mature T lymphocytes. The pattern of lymphokines produced by the hybrid cells in response to antigen was not controlled by the specific antigen receptor; both T-cell growth factor, produced only by the helper T-cell partner, and γ-interferon, produced only by the cytolytic T-cell partner, were secreted when either antigen receptor was stimulated. However, cytolytic activity appeared to be restricted to the recognition of antigen by the T-cell receptor of the cytolytic partner. Thus cytoly-sis appears to be rightly linked to the antigen receptor of the cytolytic parent but lymphokine release is not tightly linked.
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Havran, W., Fitch, F. Characterization of murine cytolytic-helper hybrid T cell clones. Nature 325, 65–67 (1987). https://doi.org/10.1038/325065a0
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DOI: https://doi.org/10.1038/325065a0
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