Molecular cloning and polymorphism of the human immune deficiency virus type 2

Article metrics

Abstract

We recently reported the isolation of a novel retrovirus, the human immune deficiency virus type 2 (HIV-2, previously named LAV-2), from patients with acquired immune deficiency syndrome (AIDS) originating from West Africa1,2. This virus is related to HIV-1, the causative agent of the AIDS epidemic now spreading in Central and East Africa, as well as the USA and Europe (see ref. 3 for review) both by its morphology and by its tropism and in vitro cytopathic effect on CD4 (T4) positive cell lines and lymphocytes. But preliminary hybridization experiments indicated that there are substantiated differences between the sequences of the two genomes2. Furthermore, the proteins of HIV-1 and HIV-2 have different sizes and their serological cross-reactivity is restricted to the major core protein, as the envelope glycoproteins of HIV-2 are not immunoprecipitated by HIV-1-positive sera1,2. We now report the molecular cloning of the complete 9.5-kilobase (kb) genome of HIV-2, the observation of restriction site polymorphism between different isolates, and a preliminary analysis of the relationship of HIV-2 with other human and simian retroviruses.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Clavel, F. et al. C.r. Acad. Sci. (Paris) 302, 485–488 (1986).

  2. 2

    Clavel, F. et al. Science 233, 343–346 (1986).

  3. 3

    Weiss, R. A. in RNA Tumor Viruses (eds Weiss, R., Teich, N., Varmus, H. & Coffin, J.) vol. II, 405–485 (Cold Spring Harbor Laboratory, New York, 1985).

  4. 4

    Alizon, M. et al. Nature 312, 757–760 (1984).

  5. 5

    Foley, G. E. et al. Cancer 18, 522–529 (1965).

  6. 6

    Wain-Hobson, S., Sonigo, P., Danos, O., Cole, S. & Alizon, M. Cell 40, 9–17 (1985).

  7. 7

    Loenen, W. A. M. & Brammar, W. J. Gene 10, 249–259 (1980).

  8. 8

    Daniel, M. D. et al. Science 228, 1201–1204 (1985).

  9. 9

    Alizon, M., Wain-Hobson, S., Montagnier, L. & Sonigo, P. Cell 46, 63–74 (1986).

  10. 10

    Kanki, P. et al. Science 232, 238–243 (1986).

  11. 11

    Barin, F. et al. Lancet ii, 1387–1389 (1985).

  12. 12

    Klatzmann, D. et al. Nature 312, 767–768 (1984).

  13. 13

    Dalgleish, A. et al. Nature 312, 763–767 (1984).

  14. 14

    Wilbur, W. J. and Lipman, D. J. Proc. natn. Acad. Sci. U.S.A. 80, 726–730 (1983).

  15. 15

    Gübler, U. & Hoffman, B. J. Gene 25, 263–269 (1983).

  16. 16

    Sanger, F., Nicklen, S. & Coulsen, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.