Abstract
The combination of complementary DNA expression and single-channel current analysis provides a powerful tool for studying the structure–function relationship of the nicotinic acetylcholine receptor (AChR) (refs 1–5). We have previously shown that AChR channels consisting of subunits from different species, expressed in the surface membrane of Xenopus oocytes, can be used to relate functional properties to individual subunits4. Here we report that, in extracellular solution of low divalent cation concentration, the bovine AChR channel has a smaller conductance than the Torpedo AChR channel. Replacement of the δ-subunit of the Torpedo AChR by the bovine δ-subunit makes the channel conductance similar to that of the bovine AChR channel. To locate the region in the δ-subunit responsible for this difference, we have constructed chimaeric δ-subunit cDNAs with different combinations of the Torpedo and bovine counterparts. The conductances of AChR channels containing chimaeric δ-subunits suggest that a region comprising the putative transmembrane segment M2 and the adjacent bend portion between segments M2 and M3 is involved in determining the rate of ion transport through the open channel.
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References
Mishina, M. et al. Nature 307, 604–608 (1984).
Mishina, M. et al. Nature 313, 364–369 (1985).
Sakmann, B. & Neher, E. A. Rev. Physiol. 46, 455–472 (1984).
Sakmann, B. et al. Nature 318, 538–543 (1985).
Mishiha, M. et al. Nature 321, 406–411 (1986).
Takai, T. et al. Nature 315, 761–764 (1985).
McLaughlin, S. G. A., Szabo, G. & Eisenman, G. J. gen. Physiol. 58, 667–687 (1971).
Hille, B., Woodhull, A. M. & Shapiro, B. I. Phil. Trans. R. Soc. Lond. B 270, 301–318 (1975).
Marty, A. J. Physiol., Paris 76, 523–527 (1980).
Noda, M. et al. Nature 302, 528–532 (1983).
Claudio, T., Ballivet, M., Patrick, J. & Heinemann, S. Proc. natn. Acad. Sci. U.S.A. 80, 1111–1115 (1983).
Devillers-Thiery, A., Giraudat, J., Bentaboulet, M. & Changeux, J.-P. Proc. natn. Acad. Sci. U.S.A. 80, 2067–2071 (1983).
Guy, H. R. Biophys. J. 45, 249–261 (1984).
Finer-Moore, J. & Stroud, R. M. Proc. natn. Acad. Sci. U.S.A. 81, 155–159 (1984).
Kubo, T. et al. Eur. J. Biochem. 149, 5–13 (1985).
Giraudat, J., Dennis, M., Heidmann, T., Chang, J.-Y. & Changeux, J.-P. Proc. natn. Acad. Sci. U.S.A. 83, 2719–2723 (1986).
Oberthür, W., Muhn, P., Baumann, H., Lottspeich, F., Wittmann-Liebold, B. & Hucho, F. EMBO J. 5, 1815–1819 (1986).
Hucho, F., Oberthür, W. & Lottspeich, F. FEBS Lett. 205, 137–142 (1986).
Konarska, M. M., Padgett, R. A., & Sharp, P. A. Cell 38, 731–736 (1984).
Methfessel, C. et al. Pflügers Arch. ges. Physiol. (in the press).
Noda, M. et al. Nature 301, 251–255 (1983).
Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
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Imoto, K., Methfessel, C., Sakmann, B. et al. Location of a δ-subunit region determining ion transport through the acetylcholine receptor channel. Nature 324, 670–674 (1986). https://doi.org/10.1038/324670a0
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DOI: https://doi.org/10.1038/324670a0
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