A new subunit of the human T-cell antigen receptor complex

Abstract

The T-cell antigen receptor binds antigen in association with a cell surface molecule encoded by the major histocompatibility complex (MHC)1. MHC restricted recognition of antigen by this receptor leads to the complex pattern of programmed gene expression that characterizes T-cell activation. The eventual understanding of human T-cell function will require the complete elucidation of the structure of the human T-cell antigen receptor. On human T cells, clonally determined, disulphide-linked α and β chains of the receptor are non-covalently and stoichiometrically associated with three additional polypeptides known as the T3 complex2,3. These receptor subunits are glycoproteins of relative molecular mass (Mr) 25,000 (25K) and 20K (γ and δ) and a non-glycosylated 20K protein (ε). Our studies of murine T cells show that the mouse T-cell antigen receptor consists of at least seven distinct polypeptide chains4–6. In addition to clonotypic α and β chains, the murine complex consists of glycoproteins of 26K and 21K and endoglycosaminidase F (endo F)-insensitive polypeptides of 25K, 21K and 16K. The latter, which we have termed ζ (zeta), exists as a homodimer within the complex. The 26K component (gp26) has been shown to be the murine analogue of the human δ chain6,7. Other cross species homologies remain to be established, however none of the described human receptor components appear similar to the murine ζ polypeptide. We report here the use of an antiserum raised against the murine ζ subunit to identify a previously unrecognized component of the human T-cell antigen receptor. This human protein is T-cell specific and biochemically similar to the murine ζ polypeptide.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Marrack, P. & Kappler, J. Adv. Immun. 38, 1–24 (1986).

    CAS  Article  Google Scholar 

  2. 2

    Borst, J., Prendiville, M. A. & Terhorst, C. Eur. J. Immun. 13, 576–580 (1983).

    CAS  Article  Google Scholar 

  3. 3

    Kanellopoulos, J. M., Wigglesworth, N. M., Owen, M. J. & Crumpton, M. J. EMBO J. 2, 1807–1814 (1983).

    CAS  Article  Google Scholar 

  4. 4

    Samelson, L. E., Harford, J. B. & Klausner, R. D. Cell 43, 223–231 (1985).

    CAS  Article  Google Scholar 

  5. 5

    Samelson, L. E., Patel, M. D., Weissman, A. M., Harford, J. B. & Klausner, R. D. Cell 46, 1083–1090 (1986).

    CAS  Article  Google Scholar 

  6. 6

    Oettgen, H. C., Pettey, C. L., Meloy, W. L. & Terhorst, C. Nature 320, 272–275 (1986).

    ADS  CAS  Article  Google Scholar 

  7. 7

    Samelson, L. E., Weissman, A. M., Robey, F. A., Berkower, I. & Klausner, R. D. J. Immun. 137, 3254–3258 (1986).

    CAS  PubMed  Google Scholar 

  8. 8

    Weiss, A. & Stobo, J. D. J. exp. Med. 160, 1284–1299 (1984).

    CAS  Article  Google Scholar 

  9. 9

    Kung, P. C., Goldstein, G., Reinherz, E. L. & Schlossman, S. F. Science 206, 347–349 (1979).

    ADS  CAS  Article  Google Scholar 

  10. 10

    Meuer, S. C. et al. J. exp. Med. 157, 705–719.

  11. 11

    Schneider, C., Sutherland, R., Neuman, R. & Greaves, M. J. biol. Chem. 257, 8516–8522 (1982).

    CAS  PubMed  Google Scholar 

  12. 12

    Samelson, L. E., Germain, R. N. & Schwartz, R. H. Proc. natn. Acad. Sci. U.S.A. 80, 6972–6976 (1983).

    ADS  CAS  Article  Google Scholar 

  13. 13

    Hildreth, J., Gotch, F., Hildreth, P. & McMichael, A. Eur. J. Immun. 13, 202–208 (1983).

    CAS  Article  Google Scholar 

  14. 14

    Rivnay, B. & Metzger, H. J. biol. Chem. 257, 12800–12808 (1982).

    CAS  PubMed  Google Scholar 

  15. 15

    Rivnay, B., Wank, S. A., Poy, G. & Metzger, H. Biochemistry 21, 6922–6927 (1982).

    CAS  Article  Google Scholar 

  16. 16

    Laemmli, U. K. Nature 227, 680–685 (1970).

    ADS  CAS  Article  Google Scholar 

  17. 17

    Falkoff, R. J. M., Zhu, L. P. & Fauci, A. S. J. Immun. 129, 97–102 (1982).

    CAS  PubMed  Google Scholar 

  18. 18

    Samelson, L. E. & Schwartz, R. H. J. Immun. 131, 2645–2650 (1983).

    CAS  PubMed  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Weissman, A., Samelson, L. & Klausner, R. A new subunit of the human T-cell antigen receptor complex. Nature 324, 480–482 (1986). https://doi.org/10.1038/324480a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing