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Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3′ end of H3 messenger RNA

Nature volume 323, pages 777–781 (1986)Cite this article

Abstract

Processing of the 3′ end of sea urchin H3 histone pre-mRNA requires conserved sequence elements and the presence of U7 snRNA. A mutation in the conserved CAAGAAAGA sequence of the H3 pre-mRNA that renders 3′ processing of this precursor defective is shown to be suppressed by a compensatory change in the U7 snRNA, restoring the base-pairing potential of the two RNAs. RNA-RNA contacts between these two molecules appear to be an essential feature of the 3′ processing reaction.

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Author notes

  1. Willi Bannwarth: Central Research Units, Hoffmann-La Roche, CH-4002, Basle, Switzerland

Authors and Affiliations

  1. Institut für Molekularbiologie II der Universität Zürich, CH-8093, Hönggerberg, Zürich, Switzerland

    Fred Schaufele, Gregory M. Gilmartin, Willi Bannwarth & Max L. Birnstiel

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  1. Fred Schaufele
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  2. Gregory M. Gilmartin
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  3. Willi Bannwarth
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  4. Max L. Birnstiel
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Schaufele, F., Gilmartin, G., Bannwarth, W. et al. Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3′ end of H3 messenger RNA. Nature 323, 777–781 (1986). https://doi.org/10.1038/323777a0

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