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Interaction of cell-type-specific nuclear proteins with immunoglobulin VH promoter region sequences

Nature volume 323, pages 548–551 (1986)Cite this article

Abstract

All human and murine immunoglobulin heavy chain variable region (VH) genes contain the sequence ATGCAAAT approximately 70 nucleotides 5′ from the site of transcription initiation1,2. This octanucleotide, in reverse orientation, is also found in all light chain variable region (VL) genes1,2, and in the immunoglobulin heavy chain transcriptional enhancer2. Transfection studies have established that this octamer is involved in the lyraphoid-specific transcription of immunoglobulin genes2–6. Octamer-containing fragments have been reported to bind a factor present in nuclear extracts of human cell lines; however, identical binding activity was detected in both B lymphoid and non-lymphoid cells7. Here we establish that nuclear extracts from distinct cell types differ in their ability to interact with octamer-containing fragments. We have also detected a DNA-protein interaction that may be involved in the cell-type specificity of immunoglobulin expression, and we have determined that a sequence upstream of the octamer participates in an interaction with a nuclear protein(s).

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References

  1. Parslow, T. G., Blair, D. L., Murphy, N. J. & Granner, D. K. Proc. natn. Acad. Sci. U.S.A. 81, 2650–2654 (1984).

    Article  ADS  CAS  Google Scholar 

  2. Falkner, F. G. & Zachau, H. G. Nature 310, 71–74 (1984).

    Article  ADS  CAS  Google Scholar 

  3. Bergman, Y., Rice, D., Grosschedl, R. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 81, 7041–7045 (1984).

    Article  ADS  CAS  Google Scholar 

  4. Mason, J. O., Williams, G. T. & Newberger, M. S. Cell 41, 479–487 (1985).

    Article  CAS  Google Scholar 

  5. Grosschedl, R. & Baltimore, D. Cell 41, 885–897 (1985).

    Article  CAS  Google Scholar 

  6. Foster, J., Stafford, J. & Queen, C. Nature 315, 423–425 (1985).

    Article  ADS  CAS  Google Scholar 

  7. Singh, H., Sen, R., Baltimore, D. & Sharp, P. Nature 319, 154–158 (1986).

    Article  ADS  CAS  Google Scholar 

  8. Gronowicz, E. S., Doss, C. A., Howard, F. D., Morrison, D. C. & Strober, S. J. Immun. 125, 976–980 (1980).

    CAS  PubMed  Google Scholar 

  9. Garner, M. M. & Revzin, A. Nucleic Acids Res. 9, 3047–3060 (1981).

    Article  CAS  Google Scholar 

  10. Fried, M. & Crothers, D. M. Nucleic Acids. Res. 9, 6505–6525 (1981).

    Article  CAS  Google Scholar 

  11. Yuan, D., Gilliam, A. C. & Tucker, P. W. Fedn Proc. 44, 2652–2659 (1985).

    CAS  Google Scholar 

  12. Yuan, D. & Tucker, P. W. J. exp. Med. 160, 564–583 (1984).

    Article  CAS  Google Scholar 

  13. Kemp, D. J., Morahan, G., Cowman, A. F. & Harris, A. W. Nature 301, 84–86 (1983).

    Article  ADS  CAS  Google Scholar 

  14. Nelson, K. J., Haimovich, J. & Perry, R. P. Molec. cell. Biol. 3, 1317–1332 (1983).

    Article  CAS  Google Scholar 

  15. Lennon, G. G. & Perry, R. P. Nature 318, 475–478 (1985).

    Article  ADS  CAS  Google Scholar 

  16. Ares, M. Jr, Mangin, M. & Weiner, A. M. Molec. cell. Biol. 5, 1560–1570 (1985).

    Article  CAS  Google Scholar 

  17. Mattaj, I. W., Leinherd, S., Jiricny, J. & DeRobertis, E. M. Nature 316, 163–167 (1985).

    Article  ADS  CAS  Google Scholar 

  18. Krol, A., Lund, E. & Dahlberg, J. E. EMBO J. 4, 1529–1535 (1985).

    Article  CAS  Google Scholar 

  19. Ciliberto, G., Buckland, R., Cortese, R. & Philipson, L. EMBO J. 4, 1537–1543 (1985).

    Article  CAS  Google Scholar 

  20. Knapp, M. R. et al. Proc. natn. Acad. Sci. U.S.A. 79, 2996–3000 (1982).

    Article  ADS  CAS  Google Scholar 

  21. Dignam, J. D., Lebovitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1489 (1983).

    Article  CAS  Google Scholar 

  22. Landolfi, N. F. & Cook, R. G. Molec. Immun. 23, 297–309 (1986).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Southwestern Medical School, University of Texas Health Science Center at Dallas, Dallas, Texas, 75235, USA

    Nicholas F. Landolfi, J. Donald Capra & Philip W. Tucker

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  1. Nicholas F. Landolfi
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  2. J. Donald Capra
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  3. Philip W. Tucker
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Landolfi, N., Capra, J. & Tucker, P. Interaction of cell-type-specific nuclear proteins with immunoglobulin VH promoter region sequences. Nature 323, 548–551 (1986). https://doi.org/10.1038/323548a0

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