Abstract
Osmotic stress can be used to estimate the internal volume change during the opening and closing of a voltage gated ionic channel. Mitochondrial voltage-dependent anion channels, from rat liver and from Neurospora, reconstituted into planar lipid bilayers show a change of 2 to 4×104 Å3 in internal volume, a large change inconsistent with a blocking or local gating model but supporting models with major closure of the channel space.
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Zimmerberg, J., Parsegian, V. Polymer inaccessible volume changes during opening and closing of a voltage-dependent ionic channel. Nature 323, 36–39 (1986). https://doi.org/10.1038/323036a0
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DOI: https://doi.org/10.1038/323036a0
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