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Deregulated expression of c-myc by murine erythroleukaemia cells prevents differentiation

Nature volume 322, pages 848–850 (1986)Cite this article

Abstract

Friend murine erythroleukaemia (F-MEL) cells are a permanent line of primitive erythroid precursors originally derived from the spleens of mice infected with the Friend strain of murine leukaemia virus1. F-MEL cells differentiate in vitro in response to various chemical inducers1,2. Concomitantly with induction, a biphasic regulation of c-myc oncogene transcripts is observed3. Within one hour of the addition of dimethyl sulphoxide (DMSO) or hypoxan-thine (Hyp), the levels of c-myc transcripts fall dramatically and remain virtually undetectable for the next few hours. Between 8 and 24 hours after induction, c-myc transcripts return to pre-induction levels and then decline again between 3 and 5 days as most of the cells undergo terminal differentiation. To explore the potential relationship between c-myc expression and F-MEL terminal differentiation, we have investigated here whether reversing the early fluctuations in c-myc transcript levels affects the ability of F-MEL cells to differentiate. We therefore constructed an amplifiable plasmid vector containing a full-length mouse c-myc complementary DNA and introduced it stably into recipient F-MEL cells. The exogenous c-myc sequences are transcribed in F-MEL cells and the transcript levels do not change significantly in response to inducing agents. The net result is continued c-myc expression following DMSO or Hyp induction and a complete or partial inhibition of F-MEL differentiation.

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Authors and Affiliations

  1. Department of Pediatrics and the Committee on Cellular and Molecular Biology, C. S. Mott Children's Hospital, University of Michigan School of Medicine, Ann Arbor, Michigan, 48109, USA

    E. V. Prochownik & J. Kukowska

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  1. E. V. Prochownik
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  2. J. Kukowska
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Prochownik, E., Kukowska, J. Deregulated expression of c-myc by murine erythroleukaemia cells prevents differentiation. Nature 322, 848–850 (1986). https://doi.org/10.1038/322848a0

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