Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication

Abstract

Dissection and reconstitution of the adenovirus DNA replication machinery has led to the discovery of two HeLa nuclear proteins which are required in conjunction with three viral proteins1–4. One of these, nuclear factor I (NF-I), recognizes an internal region of the origin between nucleotides 25 and 40 (refs 4–8) and by binding to one side of the helix stimulates the initiation reaction up to 30-fold. NFI-binding sites have been observed upstream of several cellular genes, such as chicken lysozyme9,10, human IgM11 and human c-myc12, and coincide in most cases with DNase I hypersensitive regions. Here we report the identification of a novel DNA-binding protein from HeLa nuclei, designated NF-III, that recognizes a sequence in the adenovirus origin very close to the NFI-binding site, between nucleotides 36 and 54. This sequence includes the partially conserved nucleotides TATGATAATGAG. NF-III stimulates DNA replication four- to sixfold by increasing the initiation efficiency. Potential cellular binding sites include promoter elements of the histone H2B gene, the human interferon β gene, the human and mouse immunoglobulin Vκ andVHgenes and the mammal/chicken/Xenopus laevis U1 and U2 small nuclear RNA genes. Furthermore, a subset of the herpes simplex virus immediate early promoter specific TAATGARAT elements is homologous with the adenovirus 2 (Ad-2) NFIII-binding site.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Challberg, M. D. & Kelly, T. J. A. Rev. Biochem. 51, 907–934 (1982).

    Article  Google Scholar 

  2. 2

    Sussenbach, J. S. & Van der Vliet, P. C. Curr. Top. Microbiol. Immun. 109, 53–73 (1983).

    CAS  Google Scholar 

  3. 3

    Nagata, K., Guggenheimer, R. A., Enomoto, T., Lichy, J. H. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 79, 6438–6442 (1982).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Nagata, K., Guggenheimer, R. A. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 80, 4266–4270 (1983).

    ADS  CAS  Article  Google Scholar 

  5. 5

    Rawlins, D. R., Rosenfeld, P. J., Wides, R. J., Challberg, M. D. & Kelly, T. J. Cell 37, 309–319 (1984).

    CAS  Article  Google Scholar 

  6. 6

    Guggenheimer, R. A., Stillman, B. W., Nagata, K., Tamanoi, F. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 81, 3069–3073 (1984).

    ADS  CAS  Article  Google Scholar 

  7. 7

    Leegwater, P. A. J., Van Driel, W. & Van der Vliet, P. C. EMBO J. 4, 1515–1521 (1985).

    CAS  Article  Google Scholar 

  8. 8

    De Vries, E., Van Driel, W., Tromp, M., Van Boom, J. H. & Van der Vliet, P. C. Nucleic Acids Res. 13, 4935–4952 (1985).

    CAS  Article  Google Scholar 

  9. 9

    Borgmeyer, U., Nowock, J. & Sippel, A. E. Nucleic Acids Res. 12, 4295–4311 (1984).

    CAS  Article  Google Scholar 

  10. 10

    Nowock, J., Borgmeyer, U., Püschel, A. W., Rupp, R. A. W. & Sippel, A. E. Nucleic Acids Res. 13, 2045–2061 (1985).

    CAS  Article  Google Scholar 

  11. 11

    Hennighausen, L. et al. Nature 314, 289–292 (1985).

    ADS  CAS  Article  Google Scholar 

  12. 12

    Siebenlist, U., Hennighausen, L., Battey, J. & Leder, P. Cell 37, 381–391 (1984).

    CAS  Article  Google Scholar 

  13. 13

    Sillman, B. W. Cell 35, 7–9 (1983).

    Article  Google Scholar 

  14. 14

    Pruijn, G. J. M., Kusters, H. G., Gmelig Meyling, F. H. J. & Van der Vliet, P. C. Eur. J. Biochem. 154, 363–370 (1986).

    CAS  Article  Google Scholar 

  15. 15

    Tamanoi, F. & Stillman, B. W. Proc. natn. Acad. Sci. U.S.A. 79, 2221–2225 (1982).

    ADS  CAS  Article  Google Scholar 

  16. 16

    Van Bergen, B. G. M., Van der Ley, P. A., Van Driel, W., Van Mansfeld, A. D. M. & Van der Vliet, P. C. Nucleic Acids Res. 11, 1975–1989 (1983).

    CAS  Article  Google Scholar 

  17. 17

    Leegwater, P. A. J., Van der Vliet, P. C., Rupp, R. A. W., Nowock, J. & Sippel, A. E. EMBO J. 5, 381–386 (1986).

    CAS  Article  Google Scholar 

  18. 18

    Enomoto, T., Lichy, J. H., Ikeda, J.-E. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 78, 6779–6783 (1981).

    ADS  CAS  Article  Google Scholar 

  19. 19

    Harvey, R. P., Robins, A. J. & Wells, J. R. E. Nucleic Acids Res. 10, 7851–7863 (1982).

    CAS  Article  Google Scholar 

  20. 20

    Falkner, F. G. & Zachau, H. G. Nature 310, 71–74 (1984).

    ADS  CAS  Article  Google Scholar 

  21. 21

    Fujita, T., Ohno, S., Yasumitsu, H. & Taniguchi, T. Cell 41, 489–496 (1985).

    CAS  Article  Google Scholar 

  22. 22

    Krol, A., Lund, E. & Dahlberg, J. E. EMBO J. 4, 1529–1535 (1985).

    CAS  Article  Google Scholar 

  23. 23

    Ciliberto, G., Buckland, R., Cortese, R. & Philipson, L. EMBO J. 4, 1537–1543 (1985).

    CAS  Article  Google Scholar 

  24. 24

    Whitton, J. L. & Clements, J. B. Nucleic Acids Res. 12, 2061–2079 (1984).

    CAS  Article  Google Scholar 

  25. 25

    Hay, R. T. EMBO J. 4, 421–426 (1985).

    CAS  Article  Google Scholar 

  26. 26

    Wang, K. & Pearson, G. D. Nucleic Acids Res. 13, 5173–5187 (1985).

    CAS  Article  Google Scholar 

  27. 27

    Hay, R. T. J. molec. Biol. 186, 129–136 (1985).

    CAS  Article  Google Scholar 

  28. 28

    Pearson, G. D. et al. Gene 23, 293–305 (1983).

    CAS  Article  Google Scholar 

  29. 29

    Rijnders, A. W. M., Van Bergen, B. G. M., Van der Vliet, P. C. & Sussenbach, J. S. Nucleic Acids Res. 11, 8777–8789 (1983).

    CAS  Article  Google Scholar 

  30. 30

    Tsernoglou, D., Tucker, A. D. & Van der Vliet, P. C. J. molec. Biol. 172, 237–239 (1984).

    CAS  Article  Google Scholar 

  31. 31

    Van der Vliet, P. C., Van Dam, D. & Kwant, M. M. FEBS Lett. 171, 5–8 (1984).

    ADS  CAS  Article  Google Scholar 

  32. 32

    Challberg, M. D. & Kelly, T. J. Proc. natn. Acad. Sci. U.S.A. 76, 655–659 (1979).

    ADS  CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Pruijn, G., van Driel, W. & van der Vliet, P. Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication. Nature 322, 656–659 (1986). https://doi.org/10.1038/322656a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing