Abstract
Rapid mechanical changes have been associated with electrical activity in a variety of non-muscle excitable cells1–5. Recently, mechanical changes have been reported in cochlear hair cells6–8. Here we describe electrically evoked mechanical changes in isolated cochlear outer hair cells (OHCs) with characteristics which suggest that direct electrokinetic phenomena are implicated in the response. OHCs make up one of two mechanosensitive hair cell populations in the mammalian cochlea; their role may be to modulate the micromechanical properties of the hearing organ through mechanical feedback mechanisms6–10. In the experiments described here, we applied sinusoidally modulated electrical potentials across isolated OHCs; this produced oscillatory elongation and shortening of the cells and oscillatory displacements of intracellular organdies. The movements were a function of the direction and strength of the electrical field, were inversely related to the ionic concentration of the medium, and occurred in the presence of metabolic uncouplers. The cylindrical shape of the OHCs and the presence of a system of membranes within the cytoplasm—laminated cisternae11—may provide the anatomical substrate for electrokinetic phenomena such as electro-osmosis12,13.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Iwasa, K., Tasaki, I. & Gibbons, R. C. Science 210, 338–339 (1980).
Tasaki, I. & Birne, P. M. Brain Res. 301, 265–272 (1984).
Tasaki, I. & Nakaye, T. Science 223, 411–413 (1984).
Brown, K. T. & Murakami, M. Nature 201, 626 (1964).
Hill, B. C., Schubert, E. D., Nokes, M. A. & Michelson, R. P. Science 196, 426–428 (1977).
Crawford, A. C. & Fettiplace, R. J. Physiol., Lond. 364, 359–379 (1985).
Brownell, W. E., Bader, C.R., Bertrand, D. & Ribaupierre, Y. Science 227, 194–196 (1985).
Zenner, H. P., Zimmermann, U. & Schmitt, U. Hearing Res. 18, 127–133 (1985).
Davis, H. Hearing Res. 9, 79–90 (1983).
Dallos, P. in Contemporary Sensory Neurobiology (eds Correia, M. J. & Perachio A. A.) 207–230 (Liss, New York, 1985).
Saito, K. Cell Tissue Res. 229, 467–481 (1983).
McLaughlin, S. & Mathias, R. T. J. gen. Physiol. 85, 699–728 (1985).
Poo, M. A. Rev. Biophys. Bioengng 10, 245–276 (1981).
Chance, B., Williams, G. R. & Hollunger, G. J. biol. Chem. 278, 439–444 (1961).
Epstein, M. L., Sheridan, J. D. & Johnson, R. G. Expl Cell Res. 104, 25–30 (1977).
Horwitz, B. NeuroScience 12, 887–905 (1984).
Balasubramanian, A. & McLaughlin, S. Biochim. biophys. Acta 685, 1–5 (1982).
Morrison, F. A. & Osterele, J. F. J. chem. Phys. 43, 2111–2115 (1965).
Nee, T. W. J. Chromatography 105, 231–248 (1975).
Lim, D. J. J. acoust. Soc. Am. 67, 1686–1695 (1980).
Bohne, B. A. & Carr, C. D. J. acoust. Soc. Am. 77, 153–158 (1985).
Ashmore, J. & Brownell, W. E. J. Physiol., Lond. (Abstr.) (in the press).
Brownell, W. E., Manis, P. B., Zidanic, M. & Spirou, G. A. J. acoust. Soc. Am. 74, 792–800 (1983).
Brownell, W. E. & Kachar, B. in Peripheral Auditory Mechanisms (eds Alien, J. B. et al.) (Springer, New York, 1986).
Strelioff, D., Flock, A. & Minser, K. E. Hearing Res. 18, 169–175 (1985).
Weiss, T. F. Hearing Res. 7, 353–360 (1982).
Brown, N. C. & Nutall, A. J. Physiol., Lond. 354, 625–646 (1984).
Mountain, D. C. Science 210, 71–72 (1980).
Wever, E. G. Physiol Rev. 46, 102–127 (1966).
Dallos, P. A Rev. Psychol. 32, 153–190 (1981).
Inoue, S. J. Cell Biol. 89, 346–356 (1981).
Flock, A. & Strelioff, D. Hearing Res. 15, 11–18 (1984).
Siegel, J. H. & Kim, D. O. Hearing Res. 6, 171–182 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kachar, B., Brownell, W., Altschuler, R. et al. Electrokinetic shape changes of cochlear outer hair cells. Nature 322, 365–368 (1986). https://doi.org/10.1038/322365a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/322365a0
This article is cited by
-
Single particle cryo-EM structure of the outer hair cell motor protein prestin
Nature Communications (2022)
-
Identification and characterization of amphibian SLC26A5 using RNA-Seq
BMC Genomics (2021)
-
Complex nonlinear capacitance in outer hair cell macro-patches: effects of membrane tension
Scientific Reports (2020)
-
Prestin kinetics and corresponding frequency dependence augment during early development of the outer hair cell within the mouse organ of Corti
Scientific Reports (2019)
-
The speed limit of outer hair cell electromechanical activity
HNO (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.