Abstract
Classical phenomenological approaches to the study of the mechanism of T-cell-mediated cytotoxicity1–3 have now given way to a search for molecules involved in this function; this is attempted either by subcellular and biochemical fractionation of material from cytotoxic cells4,5, or through the characterization of molecules recognized by cytotoxicity-inhibiting monoclonal antibodies (see ref. 6 for a review). Molecules having a role in cytotoxi-city may also be identified by detecting the corresponding messenger RNA transcripts. Such an approach may include, as a first step, the search for transcripts as specific as possible to cytotoxic T cells; only secondarily can their actual relevance to cytotoxicity be investigated. We report here the preparation and systematic screening of a differential complementary DNA bank, in which we detected three distinct messenger RNA transcripts (CTLA-1, CTLA-2 and CTLA-3) present in various cytotoxic T cells but not (or less so) in a range of non-cytotoxic lymphoid cells. We describe the co-inducibility of these transcripts and of cytoxicity in thymocytes and hybridoma cells, the sequence of CTLA-1 cDNA, its protein homology with serine esterases and the localization of the corresponding gene to mouse chromosome 14.
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Brunet, JF., Dosseto, M., Denizot, F. et al. The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14. Nature 322, 268–271 (1986). https://doi.org/10.1038/322268a0
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DOI: https://doi.org/10.1038/322268a0
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