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Fluorescence detection in automated DNA sequence analysis


We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.

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  1. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  2. Smith, A. J. H. Meth. Enzym. 65, 560–580 (1980).

    Article  ADS  CAS  Google Scholar 

  3. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–559 (1980).

    Article  CAS  Google Scholar 

  4. Smith, L. M., Fung, S., Hunkapiller, M. W., Hunkapiller, T. J. & Hood, L. E. Nucleic Acids Res. 13, 2399–2412 (1985).

    Article  CAS  Google Scholar 

  5. Smith, L. M., Kaiser, R. J., Sanders, J. Z. & Hood, L. E. Meth. Enzym. (in the press).

  6. Atkinson, T. & Smith, M. Oligonucleotide Synthesis: A Practical Approach (ed. Gait, M. J.) 35–81 (IRL, Oxford, 1984).

    Google Scholar 

  7. Forster, A. C., McInnes, J. L., Skingle, D. C. & Symons, R. H. Nucleic Acids Res. 13, 745–761 (1985).

    Article  CAS  Google Scholar 

  8. Chuvpilo, S. A. & Kravchenko, V. V. Sov. J. bioorg. Chem. 9, 1694–1697 (1983).

    Google Scholar 

  9. Rosenthal, A., Schwertner, S., Hahn, V. & Hunger, H. Nucleic Acids Res. 13, 1173–1184 (1985).

    Article  CAS  Google Scholar 

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Smith, L., Sanders, J., Kaiser, R. et al. Fluorescence detection in automated DNA sequence analysis. Nature 321, 674–679 (1986).

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