Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A novel cell surface molecule on early B-lineage cells

Nature volume 321, pages 616–618 (1986)Cite this article

Abstract

B cells and their antibody-secreting progeny represent one of several differentiation pathways that haematopoietic stem cells (HSC) may enter1,2. Cells representing intermediate stages between HSC and B cells have been identified in mammalian haematopoietic tissues3,4 and studied intensively over the past decade. This population of early B-lineage cells, termed pre-B, is characterized by cellular proliferation and an orderly cascade of immunoglobulin gene rearrangements5–7, a combination of events leading to the generation of clonally diverse B cells which then migrate to peripheral lymphoid tissues. It remains to be determined what elements determine the polyclonal growth of pre-B cells, how immunoglobulin gene rearrangements are regulated, and what happens to pre-B cells undergoing ‘non-productive’ immunoglobulin gene rearrangements. These issues could be resolved more easily if early B-lineage cells could be identified precisely and isolated. Here, we describe a cell surface glycoprotein that is selectively expressed by pre-B and newly formed B cells in murine haematopoietic tissues. The molecule, a homodimer formed by disulphide-linked chains of relative molecular mass (Mr) 140,000, is identified by a mouse monoclonal alloantibody called BP-1.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Cooper, M. D., Peterson, R. D. A., South, M. A. & Good, R. A. J. exp. Med. 123, 75–102 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Moore, M. A. S. & Owen, J. T. T. Lancet ii, 658–659 (1967).

    Article  Google Scholar 

  3. Osmond, D. G. & Nossal, G. J. V. Cell. Immun. 13, 117–131 (1974).

    Article  CAS  Google Scholar 

  4. Owen, J. J. T., Cooper, M. D. & Raff, M. C. Nature 249, 361–363 (1974).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Kincade, P. W. Adv. Immun. 31, 177–245 (1981).

    Article  CAS  PubMed  Google Scholar 

  6. Cooper, M. D., Velardi, A., Calvert, J. E., Gathings, W. E. & Kubagawa, H. in Progress in Immunology V (ed. Tada, T.) 603–612 (Academic, Tokyo, 1983).

    Google Scholar 

  7. Alt, F. W., Blackwell, T. K., DePinho, R. A., Reth, M. G. & Yancopoulos, G. D. Immun. Rev. 89, 5–30 (1986).

    Article  CAS  PubMed  Google Scholar 

  8. Coffman, R. L. & Weissman, I. L. Nature 289, 681–683 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Kincade, P. W., Lee, G., Watanabe, T., Sun, L. & Scheid, M. P. J. Immun. 127, 2261–2268 (1981).

    Google Scholar 

  10. McKearn, J. P., Baum, C. & Davie, J. M. J. Immun. 132, 332–339 (1984).

    CAS  PubMed  Google Scholar 

  11. Paige, C. J., Skarvalt, H. & Sauter, H. J. Immun. 134, 3699–3704 (1985).

    CAS  PubMed  Google Scholar 

  12. Pillemer, E., Whitlock, C. & Weissman, I. L. Proc. natn. Acad. Sci. U.S.A. 81, 4434–4438 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Bonhomme, F. et al. Biochem. Genet. 22, 275–303 (1984).

    Article  CAS  PubMed  Google Scholar 

  14. Alt, F., Rosenberg, N., Lewis, S., Thomas, E. & Baltimore, D. Cell 27, 381–390 (1981).

    Article  CAS  PubMed  Google Scholar 

  15. Shulman, M., Wilde, C. D. & Kohler, G. Nature 276, 269–270 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Gathings, W. E., Lawton, A. R. & Cooper, M. D. Eur. J. Immun. 7, 804–810 (1977).

    Article  CAS  Google Scholar 

  17. Williams, A. F. Nature 314, 579–580 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  18. Springer, T. G., Galfre, G., Secher, D. S. & Milstein, C. Eur. J. Immun. 9, 301–304 (1979).

    Article  CAS  Google Scholar 

  19. Bergman, Y., Haimovich, J. & Melchers, F., Eur. J. Immun. 8, 574–579 (1977).

    Article  Google Scholar 

  20. Knapp, M. R. et al. J. Immun. 123, 992–999 (1979).

    CAS  PubMed  Google Scholar 

  21. Maki, R., Kearney, J., Paige, C. & Tonegawa, S. Science 209, 1366–1369 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Perry, R. P., Kelley, D. E., Coleclough, C. & Kearney, J. F. Proc. natn. Acad. Sci. U.S.A. 78, 247–251 (1981).

    Article  ADS  CAS  Google Scholar 

  23. Burrows, P. D., LeJeune, M. & Kearney, J. F. Nature 20, 838–841 (1979).

    Article  ADS  Google Scholar 

  24. Burrows, P. D., Beck-Engeser, G. B. and Wabl, M. R. Nature 306, 243–246 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Goding, J. W. J. Immun. 124, 2082–2088 (1980).

    CAS  PubMed  Google Scholar 

  26. Chen, C. H., Chanh, T. C. & Cooper, M. D. Eur. J. Immun. 14, 385–391 (1984).

    Article  CAS  Google Scholar 

  27. Weber, K. & Osborn, M. J. biol. Chem. 244, 4406–4412 (1969).

    CAS  PubMed  Google Scholar 

  28. Bordier, C. J. biol. Chem. 256, 1604–1607 (1981).

    CAS  PubMed  Google Scholar 

  29. Elder, J. H. & Alexander, S. Proc. natn. Acad. Sci. U.S.A. 79, 4540–4544 (1982).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cellular Immunobiology Unit, Department of Pediatrics and Microbiology,

    Max D. Cooper & David Mulvaney

  2. The Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA

    Max D. Cooper & David Mulvaney

  3. Department of Immunology, Pasteur Institute, Paris, Cedex 15, France

    Antonio Coutinho & Pierre-André Cazenave

Authors
  1. Max D. Cooper
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David Mulvaney
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Antonio Coutinho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pierre-André Cazenave
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cooper, M., Mulvaney, D., Coutinho, A. et al. A novel cell surface molecule on early B-lineage cells. Nature 321, 616–618 (1986). https://doi.org/10.1038/321616a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/321616a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing