Letter | Published:

In vitro osteoclast generation from different bone marrow fractions, including a highly enriched haematopoietic stem cell population

Nature volume 321, pages 7981 (01 May 1986) | Download Citation

Subjects

Abstract

It is well established that the osteoclast is formed by fusion of post-mitotic, mononuclear precursors1 derived from circulating progenitor cells2. However, the precise haematopoietic origin of the osteoclast is unknown. We have investigated this here by fractionating mouse bone marrow and isolating haematopoietic stem cells using a three-step method combining equilibrium density centrifugation and two fluorescence-activated cell sortings (FACS)3, and have tested the ability of each bone marrow fraction, including highly purified haematopoietic stem cells, to generate osteoclasts during co-culture with preosteoclast-free embryonic long bones4,5. The osteoclast-forming capacity was found to increase with increasing stem cell purity. On the other hand, the culture time needed for osteoclast formation also increased with purification, suggesting the presence of progressively more immature progenitor cells. The pluripotent haematopoietic stem cell fractions with the highest purity needed preincubation with a stem cell-activating factor (interleukin-3) to activate the predominantly quiescent stem cells in vitro.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , , , & Lab. Invest. 53, 72–79 (1985).

  2. 2.

    J. oral Path. 12, 226–256 (1983).

  3. 3.

    , , , & J. exp. Med. 159, 1576–1590 (1984).

  4. 4.

    et al. J. exp. Med. 156, 1604–1614 (1982).

  5. 5.

    & Devl Biol. 95, 429–438 (1983).

  6. 6.

    , & Expl Hemat. 7, 541–553 (1979).

  7. 7.

    & J. cell. Physiol. 102, 233–243 (1980).

  8. 8.

    & Stem Cells 1, 240–249 (1981).

  9. 9.

    , & Leukemia Res. 6, 81–88 (1982).

  10. 10.

    & Radiat. Res. 14, 213–222 (1962).

  11. 11.

    , & Cell Tissue Kinet. 3, 355–362 (1970).

  12. 12.

    & Cell Tissue Kinet. 12, 161–175 (1979).

  13. 13.

    , & Expl Hemat. 12, Suppl. 13, abstr. 3 (1984).

  14. 14.

    & Nature 258, 325–327 (1975).

  15. 15.

    J. exp. Med. 142, 651–663 (1975).

  16. 16.

    , & Nature 283, 669–670 (1980).

  17. 17.

    & Immunobiology 161, 193–203 (1982).

  18. 18.

    , & Expl Hemat. 9, 644–655 (1981).

  19. 19.

    , , & Anat. Rec. (in the press).

Download references

Author information

Affiliations

  1. Laboratory of Cell Biology and Histology, University of Leiden, Rijnsburgerweg 10, 2333 AA Leiden, The Netherlands

    • Ben A. A. Scheven
    •  & Peter J. Nijweide
  2. Radiobiological Institute TNO, Lange Kleiweg 151, 2288 GJ Rijswijk, The Netherlands

    • Jan W. M. Visser

Authors

  1. Search for Ben A. A. Scheven in:

  2. Search for Jan W. M. Visser in:

  3. Search for Peter J. Nijweide in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/321079a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.