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cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines

Nature volume 320, pages 695–699 (1986)Cite this article

Abstract

The amino-acid sequence of the precursor of the human tumour cell line-derived platelet-derived growth factor (PDGF) A-chain has been deduced from complementary DNA clones and the gene localized to chromosome 7. The protein shows extensive homology to the PDGF B-chain precursor. Expression of the PDGF A-chain gene is independent of that of the PDGF B-chain in a number of human tumour cell lines, and secretion of a PDGF-like growth factor of relative molecular mass 31,000 correlates with expression of A- but not B-chain messenger RNA.

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Author information

Author notes

  1. Carl-Henrik Heldin

    Present address: Ludwig Institute for Cancer Research, Biomedicum, 5-751 23, Uppsala, Sweden

  2. Peter Lind

    Present address: KabiGen AB, Strandbergsgatan 49, S-112 87, Stockholm, Sweden

Authors and Affiliations

  1. Department of Pathology, University Hospital, S-751 85, Uppsala, Sweden

    Christer Betsholtz & Bengt Westermark

  2. Department of Medical and Physiological Chemistry, Box 575, Biomedicum, S-751 23, Uppsala, Sweden

    Ann Johnsson & Carl-Henrik Heldin

  3. Wallenberg Laboratory, University of Uppsala, Box 562, S-751 22, Uppsala, Sweden

    Peter Lind

  4. Chiron Corporation, 4560 Horton Street, Emeryville, California, 94608, USA

    Mickey S. Urdea

  5. Department of Human Genetics, Roswell Park Memorial Institute, Buffalo, New York, 14263, USA

    Roger Eddy & Thomas B. Shows

  6. Transplantation Biology Division, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ, UK

    Karen Philpott & Andrew L. Mellor

  7. Molecular Medicine Research Group, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ, UK

    Timothy J. Knott & James Scott

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Betsholtz, C., Johnsson, A., Heldin, CH. et al. cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. Nature 320, 695–699 (1986). https://doi.org/10.1038/320695a0

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