Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci

Nature volume 320, pages 549–551 (1986)Cite this article

Abstract

Specific chromosomal aberrations are associated with specific types of cancer (for review see ref. 1). The distinctiveness of each association has led to the belief that these chromosomal aberrations are clues to oncogenic events or to the state of differentiation in the malignant cell type2–6. Malignancies of T lymphocytes demonstrate such an association characterized most frequently by structural translocations or inversions of chromosomes 7 and 14 (refs 7–9). Analyses of these chromosomally marked tumours at the molecular level may therefore provide insight into the aetiology of the cancers as well as the mechanisms by which chromosomes break and rejoin. Here we report such an analysis of the tumour cell line SUP-T1 derived from a patient with childhood T-cell lymphoma carrying an inversion of one chromosome 14 between bands qll.2 and q32.3, that is10, inv(14) (qll.2; q32.2). These are the same chromosomal bands to which the T-cell receptor α-chain11,12 (14qll.2) and the immunoglobulin heavy-chain locus13 (14q32.3) have been assigned. Our analysis reveals that this morphological inversion of chromosome 14 was mediated by a site-specific recombination event between an immunoglobulin heavy-chain variable region (Ig VH) and a T-cell receptor (TCR) α-chain joining segment (TCR Jα). S1 nuclease analysis shows that this hybrid gene is transcribed into poly(A)+ RNA.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Yunis, J. J. Science 221, 227–236 (1983).

    Article  ADS  CAS  Google Scholar 

  2. Klein, G. Nature 294, 313–318 (1981).

    Article  ADS  CAS  Google Scholar 

  3. Yunis, J. J. & Soreng, A. L. Science 226, 1199–1204 (1984).

    Article  ADS  CAS  Google Scholar 

  4. Chaganti, R. S. K. Blood 62, 515–524 (1983).

    CAS  PubMed  Google Scholar 

  5. Kirsch, I. R., Brown, J. A., Lawrence, J., Korsmeyer, S. J. & Morton, C. C. Cancer Genet. Cytogenet. 18, 159–172 (1985).

    Article  CAS  Google Scholar 

  6. Kirsch, I. R. et al. in Genes and Cancer, 583–587 (Liss, New York, 1984).

    Google Scholar 

  7. Zech, L., Hammarstrom, L. & Smith, C. I. E. Int. J. Cancer 32, 431 (1981).

    Article  Google Scholar 

  8. Zech, L. et al. Nature 308, 858–860 (1984).

    Article  ADS  CAS  Google Scholar 

  9. Hecht, F., Morgan, R., Gemmill, R. M., Hecht, B. K. M. & Smith, S. D. New Engl. J. Med. 313, 758–759 (1985).

    CAS  PubMed  Google Scholar 

  10. Hecht, F., Morgan, R., Hecht, B. K. M. & Smith, S. D. Science 226, 1445–1447 (1984).

    Article  ADS  CAS  Google Scholar 

  11. Croce, C. M. et al. Science 227, 1044–1047 (1985).

    Article  ADS  CAS  Google Scholar 

  12. Caccia, N. et al. J. exp. Med. 161, 1255–1260 (1985).

    Article  CAS  Google Scholar 

  13. Kirsch, I. R., Morton, C. C., Nakahara, K. & Leder, P. Science 216, 301–303 (1982).

    Article  ADS  CAS  Google Scholar 

  14. Yoshikai, Y. et al. Nature 316, 837–840 (1985).

    Article  ADS  CAS  Google Scholar 

  15. Erikson, J., Williams, D. L., Finan, J., Nowell, P. C. & Croce, C. M. Science 229, 784–786 (1985).

    Article  ADS  CAS  Google Scholar 

  16. Lewis, W. H., Michalopoulos, E. E., Williams, D. L., Minden, M. D. & Mak, T. W. Nature 317, 544–546 (1985).

    Article  ADS  CAS  Google Scholar 

  17. Leder, P. Scient. Am. 246 (5), 102–115 (1982).

    Article  CAS  Google Scholar 

  18. Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. in Sequences of Proteins of Immunological Interest, 109–110 (US Department of Health and Human Services, Washington, DC, 1983).

    Google Scholar 

  19. Yanagi, Y., Chan, A., Chin, B., Minden, M. & Mak, T. W. Proc. natn. Acad. Sci. U.S.A. 82, 3430–3434 (1985).

    Article  ADS  CAS  Google Scholar 

  20. McBride, O. W. et al. Nucleic Acids Res. 10, 8155–8170 (1982).

    Article  CAS  Google Scholar 

  21. Erikson, J., Finan, J., Nowell, P. C. & Croce, C. M. Proc. natn. Acad. Sci. U.S.A. 79, 5611–5615 (1982).

    Article  ADS  CAS  Google Scholar 

  22. Berk, A. & Sharp, P. Cell 12, 721–732 (1977).

    Article  CAS  Google Scholar 

  23. Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).

    Article  ADS  CAS  Google Scholar 

  24. Sakano, H., Huppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).

    Article  ADS  CAS  Google Scholar 

  25. Sakano, H., Maki, R., Kurosawa, Y., Roder, W. & Tonegawa, S. Nature 286, 676–683 (1980).

    Article  ADS  CAS  Google Scholar 

  26. Early, P., Huang, H., Davis, M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).

    Article  CAS  Google Scholar 

  27. Sakano, H., Kurosawa, Y., Weigert, M. & Tonegawa, S. Nature 290, 562–565 (1981).

    Article  ADS  CAS  Google Scholar 

  28. Hayday, A. C. et al. Nature 316, 828–832 (1985).

    Article  ADS  CAS  Google Scholar 

  29. Winoto, A., Mjolsness, S. & Hood, L. Nature 316, 832–836 (1985).

    Article  ADS  CAS  Google Scholar 

  30. Chien, Y., Gascoigne, N. R. J., Kavaler, J., Lee, N. E. & Davis, M. M. Nature 309, 322–326 (1984).

    Article  ADS  CAS  Google Scholar 

  31. Gascoigne, N. R. J., Chien, Y., Becker, D. M., Kavaler, J. & Davis, M. M. Nature 310, 387–391 (1984).

    Article  ADS  CAS  Google Scholar 

  32. Tsujimoto, Y., Finger, L. R., Yunis, J., Nowell, P. C. & Croce, C. M. Science 226, 1097–1099 (1984).

    Article  ADS  CAS  Google Scholar 

  33. Bakhshi, A. et al. Cell 41, 899–906 (1985).

    Article  CAS  Google Scholar 

  34. Tsujimoto, Y. et al. Nature 315, 340–343 (1985).

    Article  ADS  CAS  Google Scholar 

  35. McCaw, B. K., Hecht, F., Harnden, D. G. & Teplitz, R. L. Proc. natn. Acad. Sci. U.S.A. 72, 2071–2075 (1975).

    Article  ADS  CAS  Google Scholar 

  36. Kohn, P. H., Whang-Peng, J. & Levis, W. R. Cancer Genet. Cytogenet. 6, 289–302 (1982).

    Article  CAS  Google Scholar 

  37. Taylor, A. M. R. in Ataxia-telangiectasia. A Cellular Link Between Cancer Neuropathy and Immunodeficiency (eds Bridges, B. A. & Harnden, D. G.) 53–81 (Wiley, New York, 1982).

    Google Scholar 

  38. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  39. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. NCI-Navy Medical Oncology Branch, National Cancer Institute, Naval Hospital, Bethesda, 20814, USA

    Christopher T. Denny, Gregory F. Hollis & Ilan R. Kirsch

  2. Pediatric Branch, National Cancer Institute, Naval Hospital, Bethesda, 20814, USA

    Christopher T. Denny

  3. Ontario Cancer Institute, University of Toronto, Toronto, Ontario, Canada, M5S 1A1

    Yasunobu Yoshikai & Tak W. Mak

  4. Children's Hospital of Stanford, Palo Alto, California, 94305, USA

    Stephen D. Smith

Authors
  1. Christopher T. Denny
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasunobu Yoshikai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tak W. Mak
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stephen D. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gregory F. Hollis
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ilan R. Kirsch
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Denny, C., Yoshikai, Y., Mak, T. et al. A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci. Nature 320, 549–551 (1986). https://doi.org/10.1038/320549a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/320549a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing