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Blue light-dependent proton extrusion by guard-cell protoplasts of Vicia faba

Abstract

In plant leaves, light regulation of stomatal movement, and hence of gas exchange1, involves a non-photosynthetic photosensory system sensitive to blue light2–6, which transduces many other plant movement responses. The exact nature of the primary photo-reactions of this system is still not known7. Stomatal opening is, in general, based on swelling of the guard cells driven by ion uptake1, and it has been suggested that energy for such uptake may be provided by a chemi-osmotic extrusion of protons across the plasmalemma1,8,9. We report here that guard-cell protoplasts from Vicia faba, irradiated continuously with photosynthetically saturating, high-fluence-rate red light, extrude H+ ions in response to a short pulse (30s) of blue light. The response is relatively long-lived, such that a high rate of H+ extrusion continues for several minutes after the pulse stimulation, indicating that continuous input of blue light energy is not required for a sustained response. The kinetic relation between pulse input and response output matched closely that found for the stomatal response in intact leaves6. In agreement with the chemi-osmotic hypothesis, activation of an electrogenic plasmalemma H+-ATPase is postulated as the cause of blue light-induced H+ extrusion.

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References

  1. Zeiger, E. A Rev. Pl. Physiol. 34, 441–475 (1983).

    CAS  Article  Google Scholar 

  2. Hsiao, T. C., Allaway, W. E. & Evans, L. T. Pl. Physiol. 51, 82–88 (1973).

    CAS  Article  Google Scholar 

  3. Zeiger, E. & Hepler, P. K. Science 196, 887–889 (1977).

    ADS  CAS  Article  Google Scholar 

  4. Ogawa, T., Ishikawa, H., Shimada, K. & Shibata, K. Planta 142, 61–65 (1978).

    CAS  Article  Google Scholar 

  5. Schwartz, A. & Zeiger, E. Planta 161, 129–136 (1984).

    CAS  Article  Google Scholar 

  6. Iino, M., Ogawa, T. & Zeiger, E. Proc. natn. Acad. Sci. U.S.A. 82, 8019–8023 (1985).

    ADS  CAS  Article  Google Scholar 

  7. Senger, H (ed.) Blue Light Effects in Biological Systems (Springer, Berlin, 1984).

  8. Gepstein, S., Jacobs, M. & Taiz, L. Pl. Sci. Lett. 28, 63–72 (1982).

    CAS  Article  Google Scholar 

  9. Zeiger, E., Moody, W., Hepler, P. K. & Varela, F. Nature 270, 270–271 (1977).

    ADS  Article  Google Scholar 

  10. Shimazaki, K. & Zeiger, E. Pl. Physiol. 78, 211–214 (1985).

    CAS  Article  Google Scholar 

  11. Shimazaki, K., Gotow, K. & Kondo, N. Pl. Cell Physiol. 23, 871–879 (1982).

    CAS  Google Scholar 

  12. Raschke, K. & Humble, G. D. Planta 115, 47–57 (1973).

    CAS  Article  Google Scholar 

  13. Neumann, J. & Levine, R. P. Pl. Physiol. 47, 700–704 (1971).

    CAS  Article  Google Scholar 

  14. Hornberg, C. & Weiler, E. W. Nature 310, 321–324 (1984).

    ADS  CAS  Article  Google Scholar 

  15. Kowallik, W. A. Rev. Pl. Physiol. 33, 51–72 (1982).

    CAS  Article  Google Scholar 

  16. Assmann, S. M., Simoncini, L. & Schroeder, J. I. Nature 318, 285–287 (1985).

    ADS  CAS  Article  Google Scholar 

  17. Spanswick, R. M. A Rev. Pl. Physiol. 32, 267–289 (1981).

    CAS  Article  Google Scholar 

  18. Lew, R. R. & Spanswick, R. M. Pl. Physiol. 75, 1–6 (1984).

    CAS  Article  Google Scholar 

  19. Marré, E. A Rev. Pl. Physiol. 30, 273–288 (1979).

    ADS  Article  Google Scholar 

  20. Balke, N. E. & Hodges, T. K. Pl. Sci. Lett 10, 319–325 (1977).

    CAS  Article  Google Scholar 

  21. Shimazaki, K., Gotow, K., Sakaki, T. & Kondo, N. Pl. Cell Physiol. 24, 1049–1056 (1983).

    CAS  Google Scholar 

  22. Arnon, D. Pl. Physiol 24, 1–15 (1949).

    CAS  Article  Google Scholar 

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Shimazaki, K., Iino, M. & Zeiger, E. Blue light-dependent proton extrusion by guard-cell protoplasts of Vicia faba. Nature 319, 324–326 (1986). https://doi.org/10.1038/319324a0

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