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Role of acetylcholine receptor subunits in gating of the channel

Nature volume 318, pages 538–543 (1985)Cite this article

Abstract

The Torpedo and calf acetylcholine receptors and hybrids composed of subunits from the two species have been produced in Xenopus oocytes by the use of the cloned complementary DNAs. Single-channel current measurements indicate that these receptors form channels of similar conductance but with different gating behaviour.

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References

  1. Katz, B. & Miledi, R. J. Physiol., Lond. 224, 665–699 (1972).

    Article  CAS  Google Scholar 

  2. Sakmann, B. & Neher, E. A. Rev.Physiol. 46, 455–472 (1984).

    Article  CAS  Google Scholar 

  3. Karlin, A. Cell Surface Rev. 6, 191–260 (1980).

    CAS  Google Scholar 

  4. Changeux, J.-P. Harvey Lect. 75, 85–254 (1981).

    CAS  Google Scholar 

  5. Conti-Tronconi, B. M. & Raftery, M. A. A. Rev. Biochem. 51, 491–530 (1982).

    Article  CAS  Google Scholar 

  6. Noda, M. et al. Nature 299, 793–797 (1982).

    Article  ADS  CAS  Google Scholar 

  7. Noda, M. et al. Nature 301, 251–255 (1983).

    Article  ADS  CAS  Google Scholar 

  8. Noda, M. et al. Nature 302, 528–532 (1983).

    Article  ADS  CAS  Google Scholar 

  9. Claudio, T., Ballivet, M., Patrick, J. & Heinemann, S. Proc. natn. Acad. Sci. U.S.A. 80, 1111–1115 (1983).

    Article  ADS  CAS  Google Scholar 

  10. Sumikawa, K. et al. Nucleic Acids Res. 10, 5809–5822 (1982).

    Article  CAS  Google Scholar 

  11. Devillers-Thiery, A., Giraudat, J., Bentaboulet, M. & Changeux, J.-P. Proc. natn. Acad. Sci U.S.A. 80, 2067–2071 (1983).

    Article  ADS  CAS  Google Scholar 

  12. Noda, M. et al. Nature 305, 818–823 (1983).

    Article  ADS  CAS  Google Scholar 

  13. Tanabe, T. et al. Eur. J. Biochem. 144, 11–17 (1984).

    Article  CAS  Google Scholar 

  14. Takai, T. et al. Eur. J. Biochem. 143, 109–115 (1984).

    Article  CAS  Google Scholar 

  15. Kubo, T. et al. Eur. J. Biochem. 149, 5–13 (1985).

    Article  CAS  Google Scholar 

  16. Shibahara, S. et al. Eur. J. Biochem. 146, 15–22 (1985).

    Article  CAS  Google Scholar 

  17. LaPolla, R. J., Mayne, K. M. & Davidson, N. Proc. natn. Acad. Sci. U.S.A. 81, 7970–7974 (1984).

    Article  ADS  CAS  Google Scholar 

  18. Nef, P., Mauron, A., Stalder, R., Alliod, C. & Ballivet, M. Proc. natn. Acad. Sci. U.S.A. 81, 7975–7979 (1984).

    Article  ADS  CAS  Google Scholar 

  19. Takai, T. et al. Nature 315, 761–764 (1985).

    Article  ADS  CAS  Google Scholar 

  20. Mishina, M. et al. Nature 307, 604–608 (1984).

    Article  ADS  CAS  Google Scholar 

  21. Mishina, M. et al. Nature 313, 364–369 (1985).

    Article  ADS  CAS  Google Scholar 

  22. Green, M. R., Maniatis, T. & Melton, D. A. Cell 32, 681–694 (1983).

    Article  CAS  Google Scholar 

  23. Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).

    Article  CAS  Google Scholar 

  24. Neher, E. & Sakmann, B. Proc. natn. Acad. Sci. U.S.A. 72, 2140–2144 (1975).

    Article  ADS  CAS  Google Scholar 

  25. Adams, P. R. & Sakmann, B. J. Physiol., Lond. 283, 621–644 (1978).

    Article  CAS  Google Scholar 

  26. Colquhoun, D. in Drug Receptors (ed. Rang. H. P.) 149–182 (Macmillan, London, 1973).

    Book  Google Scholar 

  27. Eisenman, G. Biophys. J. 2, Suppl. 2, 259–323 (1962).

    Article  ADS  CAS  Google Scholar 

  28. Guy, H. R. Biophys. J. 45, 249–261 (1984).

    Article  ADS  CAS  Google Scholar 

  29. Finer-Moore, J. & Stroud, R. M. Proc. natn. Acad. Sci. U.S.A. 81, 155–159 (1984).

    Article  ADS  CAS  Google Scholar 

  30. Colquhoun, D. & Sakmann, B. J. Physiol., Lond. (in the press).

  31. Colquhoun, D. & Sakmann, B. Nature 294, 464–466 (1981).

    Article  ADS  CAS  Google Scholar 

  32. Sytkowski, A. J., Vogel, Z. & Nirenberg, M. W. Proc. natn. Acad. Sci. U.S.A. 70, 270–274 (1973).

    Article  ADS  CAS  Google Scholar 

  33. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sei. U.S.A 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  34. Gurdon, J. B. The Control of Gene Expression in Animal Development (Clarendon, Oxford, 1974).

    Google Scholar 

  35. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers Arch. ges. Physiol. 391, 85–100 (1981).

    Article  CAS  Google Scholar 

  36. Colquhoun, D. & Sigworth, F.J. in Single Channel Recording (eds Sakmann, B. & Neher, E.) 191–263 (Plenum, New York, 1983).

    Book  Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für biophysikalische Chemie, D-3400, Göttingen, FRG

    Bert Sakmann, Christoph Methfessel & Tomoyuki Takahashi

  2. Departments of Medical Chemistry and Molecular Genetics and *Physiology, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Masayoshi Mishina, Tomoyuki Takahashi, Toshiyuki Takai, Masaaki Kurasaki, Kazuhiko Fukuda & Shosaku Numa

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  1. Bert Sakmann
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  2. Christoph Methfessel
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  3. Masayoshi Mishina
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  4. Tomoyuki Takahashi
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  5. Toshiyuki Takai
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  6. Masaaki Kurasaki
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  8. Shosaku Numa
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Sakmann, B., Methfessel, C., Mishina, M. et al. Role of acetylcholine receptor subunits in gating of the channel. Nature 318, 538–543 (1985). https://doi.org/10.1038/318538a0

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