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Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line

Nature volume 318, pages 73–75 (1985)Cite this article

Abstract

The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha7–MSV) on various types of cells in vitro are well documented1–8. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones9,10. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.

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Authors and Affiliations

  1. Laboratory of Molecular Oncology, Institute of Physical and Chemical Research, Wako-shi, Saitama, 351-01, Japan

    Makoto Noda, Ding-gan Liu & Yoji Ikawa

  2. Department of Microbiology, Keio University School of Medicine, Shinjuku, Tokyo, 160, Japan

    Makoto Noda, Minoru Ko & Toshiya Takano

  3. Department of Neurochemistry, Mitsubishi Kasei Institute of Life Sciences, Machida-shi, Tokyo, 194, Japan

    Akihiko Ogura & Takehiko Amano

Authors
  1. Makoto Noda
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  2. Minoru Ko
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  6. Toshiya Takano
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  7. Yoji Ikawa
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Noda, M., Ko, M., Ogura, A. et al. Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line. Nature 318, 73–75 (1985). https://doi.org/10.1038/318073a0

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