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A mouse homoeo box gene is expressed during embryogenesis and in adult kidney

Nature volume 317, pages 745–748 (1985)Cite this article

Abstract

The homoeo box sequence of Drosophila is an element located in several genes that regulate segmentation and segment identity1; it has homologues in the genomes of vertebrate species2 and a number of homoeo box-containing recombinant DNA clones have been isolated from Xenopus, man and mouse3–9. In the present study we have isolated from a library of murine DNA a recombinant λ clone (H24.1) which contains a sequence closely homologous to the homoeo box within the Drosophila Antennapedia (Antp) gene. The protein sequence of the homoeo domain is identical to that encoded by Hu-1, one of a pair of closely linked homoeo boxes in the human genome5. We have used a sensitive RNase protection assay to examine transcription of the H24.1 gene during mouse development, and in adult tissues. We report that the gene is transcribed from as early as 7.5 days post coitum (p.c.), with maximum expression at days 11.5 and 12.5 p.c. The transcript is enriched in embryonic spinal cord and brain at day 12.5 p.c., and in adult kidney.

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Author notes

  1. Paul Schofield

    Present address: Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK

Authors and Affiliations

  1. MRC Mammalian Development Unit, Wolfson House, 4 Stephenson Way, London, NW1 2HE

    Ian J. Jackson

  2. Laboratory of Molecular Embryology, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

    Paul Schofield & Brigid Hogan

Authors
  1. Ian J. Jackson
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  2. Paul Schofield
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  3. Brigid Hogan
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Jackson, I., Schofield, P. & Hogan, B. A mouse homoeo box gene is expressed during embryogenesis and in adult kidney. Nature 317, 745–748 (1985). https://doi.org/10.1038/317745a0

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