Abstract
Flies and moths are approximately as distant phylogenetically as are mammals and birds. In terms of morphology, physiology and biochemistry, the complex proteinaceous eggshell or chorion differs substantially in these two insect groups, which are typified by Drosophila melanogaster and Bombyx mori. The major chorion proteins of moths are encoded by two families of genes, A and B, which have no obvious homologues in flies1. Unlike Drosophila, where chorion genes are oriented in tandem, moths show mostly chorion gene pairs (A plus B) that are divergently transcribed and coordinately expressed. The 5′ ends of the paired genes are separated by a DNA segment of only 300±50 base pairs, which may well include at least some of the cis-regulatory elements necessary for gene expression. Despite these differences, we have tested whether moth chorion genes might be expressed in flies. Cloned DNA fragments bearing moth chorion genes were introduced into the Drosophila germ line by P-element-mediated transformation2,3. Analysis of RNAs from transformed lines revealed that the genes are expressed with correct sex, tissue and temporal specificity, resulting in the accumulation of abundant moth chorion transcripts in late fly follicles.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kafatos, F. C. in Gene Structure and Regulation in Development. 41st Symp. Soc. dev. Biol. (eds Subtelny, S. & Kafatos, F. C.) 33–61 (Liss, New York, 1983).
Spradling, A. C. & Rubin, G. M. Science 218, 341–347 (1982).
Rubin, G. M. & Spradling, A. C. Science 218, 348–353 (1982).
Rubin, G. M. & Spradling, A. C. Nucleic Acids Res. 11, 6341–6351 (1983).
Orr, W., Komitopoulou, K. & Kafatos, F. C. Proc. natn. Acad. Sci. U.S.A. 81, 3773–3777 (1984).
Thireos, G., Griffin-Shea, R. & Kafatos, F. C. Proc. natn. Acad. Sci. U.S.A. 77, 5789–5793 (1980).
Griffin-Shea, R., Thireos, G. & Kafatos, F. C. Devl Biol. 91, 325–336 (1982).
Riek, E. F. in The Insects of Australia, 168–186 (Melbourne University Press, Carlton, Australia, 1970).
Hinton, H. E. A. Rev. Ent. 3, 181–206 (1958).
Martynova, O. A. Rev. Ent. 6, 185–294 (1961).
Corces, V. A. et al. Proc. natn. Acad. Sci. U.S.A. 78, 7038–7041 (1981).
McKnight, G. S. et al. Cell 34, 335–341 (1983).
Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
McMaster, G. K. & Carmichael, G. C. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).
Cox, K. H. et al. Devl Biol. 101, 485–502 (1984).
Church, G. M. & Gilbert, A. Proc. natn. Acad. Sci. U.S.A. 81, 1991–1995 (1984).
King, R. C. Ovarian Development in Drosophila melanogaster (Academic, New York, 1970).
Berk, A. J. & Sharp, P. A. Cell 12, 721–730 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mitsialis, S., Kafatos, F. Regulatory elements controlling chorion gene expression are conserved between flies and moths. Nature 317, 453–456 (1985). https://doi.org/10.1038/317453a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/317453a0
This article is cited by
-
Anatomy and evolution of a DNA replication origin
Chromosoma (2021)
-
A comprehensive analysis of the chorion locus in silkmoth
Scientific Reports (2015)
-
Genome clashes in hybrids: insights from gene expression
Heredity (2007)
-
Evolution of cis-regulatory sequence and function in Diptera
Heredity (2006)
-
Gene Network Polymorphism Is the Raw Material of Natural Selection: The Selfish Gene Network Hypothesis
Journal of Molecular Evolution (2004)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.