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Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination

Abstract

A ‘rescuable’ plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human β-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.

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Smithies, O., Gregg, R., Boggs, S. et al. Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination. Nature 317, 230–234 (1985). https://doi.org/10.1038/317230a0

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