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Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination

Nature volume 317, pages 230–234 (1985)Cite this article

Abstract

A ‘rescuable’ plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human β-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.

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Authors and Affiliations

  1. Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin, 53706, USA

    Oliver Smithies, Ronald G. Gregg & Michael A. Koralewski

  2. School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, 15261, USA

    Sallie S. Boggs

  3. Center for Genetics, University of Illinois College of Medicine, Chicago, Illinois, 60612, USA

    Raju S. Kucherlapati

Authors
  1. Oliver Smithies
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  2. Ronald G. Gregg
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  3. Sallie S. Boggs
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  4. Michael A. Koralewski
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  5. Raju S. Kucherlapati
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Smithies, O., Gregg, R., Boggs, S. et al. Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination. Nature 317, 230–234 (1985). https://doi.org/10.1038/317230a0

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