Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Primary structure and transmembrane orientation of the murine anion exchange protein

Nature volume 316, pages 234–238 (1985)Cite this article

Abstract

The amino-acid sequence of murine band 3, deduced from the nucleotide sequence of a complementary DNA clone, confirms that this integral membrane glycoprotein is composed of two major structural domains which correlate with its dual functions as the anchor for the erythrocyte cytoskeleton and as a plasma membrane anion antiporter. This latter activity resides within a highly Hydrophobie domain that crosses the plasma membrane at least 12 times.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Fairbanks, G., Steck, T. L. & Wallach, D. F. H. Biochemistry 10, 2606–2617 (1971).

    Article  CAS  PubMed  Google Scholar 

  2. Weith, J. O., Andersen, O. S., Brahm, J., Bjerrum, P. H. & Borders, C. L. Phil. Trans. R. Soc. B299, 383–399 (1982).

    Article  Google Scholar 

  3. Steck, T. L. J. Cell Biol. 62, 1–19 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Macara, I. G. & Cantley, L. C. in Cell Membranes, Methods and Reviews (eds E. Elson, W. Frazier & L. Glaser) 41–87 (Plenum, New York, 1982).

    Google Scholar 

  5. Cabantchik, Z. I., Knauf, P. A. & Rothstein, A. Biochim. biophys. Acta 515, 239–302 (1978).

    Article  CAS  PubMed  Google Scholar 

  6. Knauf, P. A. Curr. Topics Membrane Transport 12, 249–363 (1979).

    Article  CAS  Google Scholar 

  7. Bennett, V. & Stenbuck, P. J. Nature 280, 468–473 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Bennett, V. & Stenbuck, P. J. J. biol. Chem. 255, 6424–6432 (1980).

    CAS  PubMed  Google Scholar 

  9. Bennett, V. J. cell. Biochem. 18, 49–65 (1982).

    Article  CAS  PubMed  Google Scholar 

  10. Shaklai, N., Yguerabide, J. & Ranney, H. M. Biochemistry 16, 5593–5592 (1977).

    Article  CAS  PubMed  Google Scholar 

  11. Strapazen, E. & Steck, T. L. Biochemistry 15, 1421–1424 (1976).

    Article  Google Scholar 

  12. Yu, J. & Steck, T. L. J biol. Chem. 250, 9176–9184 (1975).

    CAS  Google Scholar 

  13. Grinstein, S., Ship, S. & Rothstein, A. Biochim. biophys. Acta 507, 294–304 (1978).

    Article  CAS  PubMed  Google Scholar 

  14. Jennings, M. L., Lackey, M. A. & Denney, D. H. J. biol. Chem. 259, 4652–4660 (1984).

    CAS  PubMed  Google Scholar 

  15. Jennings, M. L. & Nicknish, J. S. Biochemistry 23, 6432–6436 (1984).

    Article  CAS  PubMed  Google Scholar 

  16. Braell, W. A. & Lodish, H. F. J biol. Chem. 256, 11337–11334 (1981).

    CAS  PubMed  Google Scholar 

  17. Bozzini, C. E., Barrio-Rendo, M. E., Devote, F. C. H. & Eppper, C. E. A. J. Physiol. 219, 724–728 (1970).

    CAS  Google Scholar 

  18. Spivak, J. L., Marmor, J. & Dickerman, H. W. J. Lab. clin. Med. 79, 526–540 (1972).

    CAS  PubMed  Google Scholar 

  19. Young, R. A. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 80, 1194–1198 (1983).

    Article  ADS  CAS  Google Scholar 

  20. Braell, W. A. & Lodish, H. F. Cell 28, 23–31 (1982).

    Article  CAS  PubMed  Google Scholar 

  21. Kaul, R. K., Murthy, P. S. N., Reddy, A. G., Steck, T. L. & Kohler, H. J. biol. Chem. 258, 7981–7990 (1983).

    CAS  PubMed  Google Scholar 

  22. Kozak, M. Nucleic Acids Res. 9, 5223–5252 (1981).

    Article  Google Scholar 

  23. Braell, W. A. thesis Massachusetts Inst. Technol. (1981).

  24. Drickamer, L. K. J. biol. Chem. 251, 5115–5123 (1976).

    CAS  PubMed  Google Scholar 

  25. Mawby, W. J. & Findlay, J. B. C. Biochem. J. 205, 465–475 (1983).

    Article  Google Scholar 

  26. Brock, C. J., Tanner, M. J. A. & Kempf, C. Biochem. J. 213, 577–586 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  PubMed  Google Scholar 

  28. Walder, J. A. et al. J. biol. Chem. 259, 10238–10246 (1984).

    CAS  PubMed  Google Scholar 

  29. Rao, A. J. biol. Chem. 254, 3503–3511 (1979).

    CAS  PubMed  Google Scholar 

  30. Low, P. S., Westfall, M. A., Allen, D. P. & Appell, K. C. J. biol. Chem. 259, 13070–13076 (1984).

    CAS  PubMed  Google Scholar 

  31. England, B. J., Gunn, R. B. & Steck, T. L. Biochim biophys. Acta 623, 171–182 (1980).

    Article  CAS  PubMed  Google Scholar 

  32. Eisenberg, D. A. Rev. Biochem. 53, 595–623 (1984).

    Article  CAS  Google Scholar 

  33. Steck, T. L., Ramos, B. & Strapazen, E. Biochemistry 15, 1154–1161 (1976).

    Article  CAS  Google Scholar 

  34. Schiffer, M. & Edmundson, A. B. Biophys. J. 7, 121–135 (1967).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Jennings, M. L. & Passow, H. Biochim. biophys. Acta 554, 498–519 (1979).

    Article  CAS  PubMed  Google Scholar 

  36. Finer-Moore, J. & Stroud, R. M. Proc. natn. Acad. Sci. U.S.A. 81, 155–159 (1984).

    Article  ADS  CAS  Google Scholar 

  37. Chirgwin, J. M., Przybyla, A. E., McDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  PubMed  Google Scholar 

  38. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  39. Hunyh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning Techniques: A Practical Approach (ed. Glover, D.) (IRL, Oxford, in the press).

  40. Young, R. A. & Davis, R. W. in Genetic Engineering Vol. 7 (eds Setlow, J. & Hollaender, A.) 29–41 (Plenum, New York, 1985).

    Google Scholar 

  41. Vieira, J. & Messing, J. Gene 19, 259–268 (1982).

    Article  CAS  PubMed  Google Scholar 

  42. Maniatis, T., Fritsch, E. F. & Sambrook, J. in Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  43. Gubler, U. & Hofman, B. J. Gene 25, 263–269 (1983).

    Article  CAS  PubMed  Google Scholar 

  44. Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  PubMed  Google Scholar 

  46. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  47. Biggin, M. D., Gibson, T. J. & Hong, G. F. Proc. natn. Acad. Sci. U.S.A. 80, 3963–3965 (1983).

    Article  ADS  CAS  Google Scholar 

  48. Guo, L.-H., Yang, R. C. A. & Wu, R. Nucleic Acids Res. 111, 5521–5540 (1983).

    Article  Google Scholar 

  49. Deininger, P. L. Analyt. Biochem. 129, 216–223 (1983).

    Article  CAS  PubMed  Google Scholar 

  50. Messing, J., Gronenborn, B., Muller-Hill, B. & Hofschneider, D. H. Proc. natn. Acad. Sci. U.S.A. 74, 3642–3646 (1977).

    Article  ADS  CAS  Google Scholar 

  51. Staden, R. Nucleic Acids Res. 10, 4731–4751 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. Dayhoff, M. O., Barker, W. C. & Hunt, T. L. Meth. Enzym. 91, 534–545 (1983).

    Google Scholar 

  53. Tanner, M. J. A., Williams, D. G. & Kyle, D. Biochem. J. 183, 417–427 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts, 02142, USA

    Ron R. Kopito & Harvey F. Lodish

  2. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Harvey F. Lodish

Authors
  1. Ron R. Kopito
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Harvey F. Lodish
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kopito, R., Lodish, H. Primary structure and transmembrane orientation of the murine anion exchange protein. Nature 316, 234–238 (1985). https://doi.org/10.1038/316234a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/316234a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing