Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Local degradation of fibronectin at sites of expression of the transforming gene product pp60src

Abstract

Local degradation of extracellular fibronectin, a major extracellular adhesive protein, is believed to play an important part in the migration of cells through the extracellular matrix during tumour invasion, morphogenetic movement and trophoblast implantation1–5. Fibronectin is lost from the cell surface after transformation with Rous sarcoma virus (RSV)6. By using fluorescent and radiolabelled probes covalently coupled to the surface of substrata, we have recently identified a proteolytic activity that is expressed in RSV-transformed cells and is involved in the local degradation of fibronectin at cell–substratum contact sites7. Here, we extend the relevance of these findings and gain some insight into the cellular functions of pp60src, the transforming gene product of RSV. We show that newly expressed viral pp60src is localized at the cytoplasmic surface of the cell membrane, corresponding to the cell contact sites where degradation of extracellular fibronectin occurs.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Liotta, L., Rao, C. & Barsky, S. in The role of Extracellular Matrix in Development, 357–371 (Liss, New York, 1984).

    Google Scholar 

  2. Nicolson, G. L. Biochim. biophys. Acta 695, 113–176 (1982).

    CAS  PubMed  Google Scholar 

  3. Kalebic, T., Garbisa, S., Glaser, B. & Liotta, L. A. Science 221, 281–283 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Quigley, J. P. Cell 17, 131–141 (1979).

    Article  CAS  PubMed  Google Scholar 

  5. Glass, R. H., Aggeler, J., Spindle, A., Pedersen, R. A. & Werb, Z. J. Cell Biol. 96, 1108–1116 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Hynes, R. O. Proc. natn. Acad. Sci. U.S.A. 70, 3170–3173 (1973).

    Article  ADS  CAS  Google Scholar 

  7. Chen, W.-T., Olden, K., Bernard, B. & Chu, F. J. Cell Biol. 98, 1546–1555 (1984).

    Article  CAS  PubMed  Google Scholar 

  8. David-Pfeuty, T. & Singer, S. Proc. natn. Acad. Sci. U.S.A. 77, 6687–6691 (1980).

    Article  ADS  CAS  Google Scholar 

  9. Nigg, E., Sefton, B., Hunter, T., Walter, G. & Singer, S. Proc. natn. Acad. Sci. U.S.A. 79, 5322–5326 (1982).

    Article  ADS  CAS  Google Scholar 

  10. Shriver, K. & Rohrschneider, L. J. Cell Biol. 89, 525–535 (1981).

    Article  CAS  PubMed  Google Scholar 

  11. Wang, E., Yin, H., Krueger, J., Caliguiri, L. & Tamm, I. J. Cell Biol. 98, 761–771 (1984).

    Article  CAS  PubMed  Google Scholar 

  12. Hanafusa, H. Compreh. Virol. 10, 401–483 (1977).

    Article  CAS  Google Scholar 

  13. Sefton, B., Hunter, T., Ball, E. & Singer, S. Cell 24, 165–174 (1981).

    Article  CAS  PubMed  Google Scholar 

  14. Sefton, B. & Walter, G. J. Virol. 44, 467–474 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Wang, E. & Goldberg, A. Proc. natn. Acad. Sci. U.S.A. 78, 7412–7416 (1981).

    Article  ADS  Google Scholar 

  16. Parsons, S., McCarley, D., Ely, C., Benjamin, D. & Parsons, J. J. Virol. 45, 1190–1194 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Parsons, S., McCarley, D., Ely, C., Benjamin, D. & Parsons, J. J. Virol. 52, 272–282 (1984).

    Google Scholar 

  18. Bishop, J. M., A. Rev. Biochem. 53, 301–354 (1983).

    Article  Google Scholar 

  19. Collett, M. & Erikson, R. Proc. natn. Acad. Sci. U.S.A. 75, 2021–2024 (1978).

    Article  ADS  CAS  Google Scholar 

  20. Levinson, A., Oppermann, H., Levintow, L., Varmus, H. & Bishop, J. Cell 15, 561–572 (1978).

    Article  CAS  PubMed  Google Scholar 

  21. Willingham, M., Jay, G. & Pastan, I. Cell 18, 125–134 (1979).

    Article  CAS  PubMed  Google Scholar 

  22. Oppermann, H., Levinson, A., Varmus, H., Levintow, L. & Bishop, J. Proc. natn. Acad. Sci. U.S.A. 76, 1804–1808 (1979).

    Article  ADS  CAS  Google Scholar 

  23. Rohrschneider, L. & Rosok, M. Molec. cell. Biol. 3, 731–746 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, WT., Chen, JM., Parsons, S. et al. Local degradation of fibronectin at sites of expression of the transforming gene product pp60src. Nature 316, 156–158 (1985). https://doi.org/10.1038/316156a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/316156a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing