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Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs

Nature volume 315, pages 641–647 (1985)Cite this article

Abstract

Two distinct but distantly related complementary DNAs encoding proteins sharing human interleukin-1 (IL-1) activity (termed IL-lα and IL-1β), were isolated from a macrophage cDNA library. The primary translation products of the genes are 271 and 269 amino acids long, although expression in Escherichia coli of the carboxy-terminal 159 and 153 amino acids produces IL-1 biological activity.

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Authors and Affiliations

  1. Department of Protein Chemistry, 51 University Street, Seattle, Washington, 98101, USA

    Carl J. March, Steven Gillis, Christopher S. Henney, Shirley R. Kronheim & Thomas P. Hopp

  2. Department of Molecular Biology, 51 University Street, Seattle, Washington, 98101, USA

    Bruce Mosley, Alf Larsen, Douglas Pat Cerretti, Gary Braedt, Virginia Price & David Cosman

  3. Cellular Immunology Immunex Corporation, 51 University Street, Seattle, Washington, 98101, USA

    Kenneth Grabstein & Paul J. Conlon

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  1. Carl J. March
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  3. Alf Larsen
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March, C., Mosley, B., Larsen, A. et al. Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315, 641–647 (1985). https://doi.org/10.1038/315641a0

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