Human transforming growth factor-α causes precocious eyelid opening in newborn mice

Abstract

Both marine and human epidermal growth factors (EGFs) are known to cause precocious opening of the eyelids in newborn mice1–3. Another set of peptides that are structurally and functionally homologous to murine and human EGFs are the murine and human type-α transforming growth factors (TGF-αs)4–7. TGF-αs have been found in many cancer cells and it has been suggested that their autocrine action may play an important part in malignant transformation8–11. In several in vitro systems murine and human TGF-αs are functionally interchangeable with murine and human EGFs6,12–14. However, the in vivo activity of the TGF-αs has not been characterized, as only small amounts of these peptides were available until recently. The cloning of the gene for human TGF-α and its expression in Escherichia coli6 now allow us to demonstrate that human TGF-α is as active as murine EGF in promoting eyelid opening in newborn mice. Furthermore, we show in a dose-dependent eyelid opening assay that human EGF is as potent as its murine homologue with respect to this biological property.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Cohen, S. J. biol. Chem. 237, 1555–1562 (1962).

    CAS  PubMed  Google Scholar 

  2. 2

    Gregory, H. Nature 257, 325–327 (1975).

    ADS  CAS  Article  Google Scholar 

  3. 3

    Cohen, S. & Carpenter, G. Proc. natn. Acad. Sci. U.S.A. 72, 1317–1321 (1975).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Marquardt, H., Hunkapiller, M. W., Hood, L. E. & Todaro, G. J. Science 223, 1079–1082 (1984).

    ADS  CAS  Article  Google Scholar 

  5. 5

    Marquardt, H. & Todaro, G. J. J. biol. Chem. 257, 5220–5225 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. 6

    Derynck, R., Roberts, A. B., Winkler, M. E., Chen, E. Y. & Goeddel, D. V. Cell 38, 287–297 (1984).

    CAS  Article  Google Scholar 

  7. 7

    Roberts, A. B., Frolik, C. A., Anzano, M. A. & Sporn, M. B. Fedn Proc. 42, 2621–2626 (1983).

    CAS  Google Scholar 

  8. 8

    De Larco, J. E. & Todaro, G. J. Proc. natn. Acad. Sci. U.S.A. 75, 4001–4005 (1978).

    ADS  CAS  Article  Google Scholar 

  9. 9

    Todaro, G. J., Fryling, C. & De Larco, J. E. Proc. natn. Acad. Sci. U.S.A. 77, 5258–5262 (1980).

    ADS  CAS  Article  Google Scholar 

  10. 10

    Sporn, M. B. & Todaro, G. J. New Engl. J. Med. 303, 878–880 (1980).

    CAS  Article  Google Scholar 

  11. 11

    Sporn, M. B. & Roberts, A. B. Nature 313, 745–747 (1985).

    ADS  CAS  Article  Google Scholar 

  12. 12

    Anzano, M. A. et al. Cancer Res. 42, 4776–4778 (1982).

    CAS  Google Scholar 

  13. 13

    Massagué, J. J. biol. Chem. 258, 13606–13613, 13614–13620 (1983).

    PubMed  PubMed Central  Google Scholar 

  14. 14

    Twardzik, D. R., Todaro, G. J. & Marquardt, H. Science 216, 894–897 (1982).

    ADS  CAS  Article  Google Scholar 

  15. 15

    Cohen, S. & Carpenter, G. A. Rev. Biochem. 48, 193–216 (1979).

    CAS  Article  Google Scholar 

  16. 16

    Hollenberg, M. D. Vitam. Horm. 37, 69–110 (1979).

    CAS  Article  Google Scholar 

  17. 17

    Smith, J. et al. Nucleic Acids Res. 10, 4467–4482 (1982).

    ADS  CAS  Article  Google Scholar 

  18. 18

    Roberts, A. B., Anzano, M. A., Lamb, L. C., Smith, J. M. & Sporn, M. B. Proc. natn. Acad. Sci. U.S.A. 78, 5339–5343 (1981).

    ADS  CAS  Article  Google Scholar 

  19. 19

    Savage, C. R. & Cohen, S. J. biol. Chem. 247, 7609–7611 (1972).

    CAS  Google Scholar 

  20. 20

    Assoian, R. K., Komoriya, A., Meyers, C. A., Miller, D. M. & Sporn, M. B. J. biol. Chem. 258, 7155–7160 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Smith, J., Sporn, M., Roberts, A. et al. Human transforming growth factor-α causes precocious eyelid opening in newborn mice. Nature 315, 515–516 (1985). https://doi.org/10.1038/315515a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.