Ca2+-dependent and Ca2+-independent phagocytosis in human neutrophils

Abstract

The phagocytic function of neutrophils is a crucial element in host defence against invading microorganisms. Two main specific receptor-mediated mechanisms operate in the phagocyte plasma membrane, one recognizing the C3b/bi fragment of complement and the other the Fc domain of immunoglobulin G (ref. 1). There is evidence that phagocytosis mediated by these receptors differs in the number and nature of the intracellular signals generated2–5. However, the mechanism by which receptor binding is transduced into a signal that generates the formation of the phagocyte pseudopod is not known, although extensive biochemical evidence has allowed the postulate that calcium ion gradients in the peripheral cytoplasm, by interacting with calcium-sensitive contractile proteins, initiate the process of engulfment6. Using the high-affinity fluorescent calcium indicator quin2 both to measure and to buffer intracellular calcium ([Ca2+]i), we show here that in human neutrophils two mechanisms of phagocytosis coexist: a [Ca2+]i-dependent and modulated phagocytosis, triggered by activation of the Fc receptor, and a [Ca2+]i-independent mechanism triggered by the activation of the C3b/bi receptors.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Silverstein, S. C., Steinman, R. M. & Cohn, Z. A. A. Rev. Biochem. 46, 665–722 (1977).

    Article  Google Scholar 

  2. 2

    Hed, J. & Stendahl, O. Immunology 45, 727–736 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. 3

    Newman, S. L. & Johnston, R. B. Jr J. exp. Med. 123, 1839–1846 (1979).

    CAS  Google Scholar 

  4. 4

    Wright, S. D. & Silverstein, S. C. J. exp. Med. 158, 2016–2023 (1983).

    CAS  Article  Google Scholar 

  5. 5

    Yamamoto, K. & Johnston, R. B. Jr J. exp. Med. 159, 406–416 (1984).

    Article  Google Scholar 

  6. 6

    Southwick, F. S. & Stossel, T. P. Semin. Hemat. 20, 305–321 (1983).

    CAS  PubMed  Google Scholar 

  7. 7

    Campbell, A. K. & Hallett, M. B. J. Physiol., Lond. 358, 537–550 (1983).

    Article  Google Scholar 

  8. 8

    Korchack, H. M. et al. J. biol. Chem. 259, 4076–4080 (1984).

    Google Scholar 

  9. 9

    Young, J. D.-E., Ko, S. S. & Cohn, Z. A. Proc. natn. Acad. Sci. U.S.A. 81, 5430–5434 (1984).

    ADS  CAS  Article  Google Scholar 

  10. 10

    Tsien, R. Y., Pozzan, T. & Rink, T. J. Nature 295, 68–71 (1982).

    ADS  CAS  Article  Google Scholar 

  11. 11

    Tsien, R. Y., Pozzan, T. & Rink, T. J. J. Cell Biol. 94, 325–334 (1982).

    CAS  Article  Google Scholar 

  12. 12

    Di Virgilio, F., Lew, P. D. & Pozzan, T. Nature 310, 691–693 (1984).

    ADS  CAS  Article  Google Scholar 

  13. 13

    Lew, P. D., Wollheim, C. B., Waltvogel, F. A. & Pozzan, T. J. Cell Biol. 99, 1212–1220 (1984).

    CAS  Article  Google Scholar 

  14. 14

    Yin, H. L. & Stossel, T. P. Nature 281, 583–585 (1979).

    ADS  CAS  Article  Google Scholar 

  15. 15

    Cheung, W. Y. Science 207, 19–27 (1980).

    ADS  CAS  Article  Google Scholar 

  16. 16

    Niggli, V., Adunyah, E. S. & Carafoli, E. J. biol. Chem. 256, 8588–8592 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. 17

    Stossel, T. P. J. Cell Biol. 58, 346–356 (1973).

    CAS  Article  Google Scholar 

  18. 18

    Stossel, T. P., Alper, C. A. & Rosen, F. S. J. exp. Med. 137, 690–705 (1973).

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lew, D., Andersson, T., Hed, J. et al. Ca2+-dependent and Ca2+-independent phagocytosis in human neutrophils. Nature 315, 509–511 (1985). https://doi.org/10.1038/315509a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.