Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross

Abstract

The introduction of cloned probes to follow the segregation of DNA restriction fragment length polymorphisms (RFLPs) has led to a revival of mendelian genetics in attempts to map the human genome1. In the mouse, however, it has often proved difficult to detect an RFLP with a DNA probe between different inbred strains of the laboratory mouse. To circumvent this problem, we have used two species, Mus musculus domesticus and Mus spretus2 which interact as sympatric species3 but can be interbred under laboratory conditions4. Because of the relative evolutionary distance between these species, they exhibit polymorphism at many more loci than do different strains of the usual M. m. domesticus laboratory mouse2. This is also observed at the DNA level when the sizes of restriction fragments encoding a specific gene are compared5. We have used these RFLPs between M. m. domesticus and M. spretus to follow the segregation of genes encoding different isoforms of myosin alkali light chains in the backcross progeny between these species and to compare this with that of other contractile protein genes. No linkage between these genes was observed.

References

  1. 1

    Solomon, E. & Goodfellow, P. Nature 306, 223–224 (1983).

    ADS  Article  Google Scholar 

  2. 2

    Bonhomme, F. et al. Biochem. Genet. 22, 275–303 (1984).

    CAS  Article  Google Scholar 

  3. 3

    Britton-Davidian, J. & Thaler, L. Biochem. Genet. 16, 213–225 (1978).

    Article  Google Scholar 

  4. 4

    Bonhomme, F., Martin, S. & Thaler, L. Experientia 34, 1140–1141 (1978).

    CAS  Article  Google Scholar 

  5. 5

    Robert, B. et al. Cell 29, 129–140 (1984).

    Article  Google Scholar 

  6. 6

    Barton, P. J. R. et al. J. biol. Chem. (submitted).

  7. 7

    Whalen, R. G. & Sell, S. Nature 286, 731–733 (1980).

    ADS  CAS  Article  Google Scholar 

  8. 8

    Barton, P. J. R., Robert, B., Fiszman, M. Y., Leader, D. P. & Buckingham, M. E. J. Muscle Res. Cell Motility (in the press).

  9. 9

    Taylor, B. A. in Origins of Inbred Mice (ed. Morse, H.) 423–438 (Academic, New York, 1978).

    Google Scholar 

  10. 10

    Genetic Variants and Strains of the Laboratory Mouse (ed. Green, M.)(Fischer, Stuttgart, 1981).

  11. 11

    Minty, A. J. et al. J. biol. Chem. 256 1008–1014 (1981).

    CAS  PubMed  Google Scholar 

  12. 12

    Minty, A. J., Alonso, S., Caravatti, M. & Buckingham, M. E. Cell 30, 185–192 (1982).

    CAS  Article  Google Scholar 

  13. 13

    Weydert, A. et al. J. biol. Chem. 258 13867–13874 (1983).

    CAS  PubMed  Google Scholar 

  14. 14

    Czosnek, H. et al. EMBO J. 1, 1299–1305 (1982).

    CAS  Article  Google Scholar 

  15. 15

    Czosnek, H. et al. EMBO J. 2, 1977–1979 (1983).

    CAS  Article  Google Scholar 

  16. 16

    Leinwand, L. A., Fournier, R. E. K., Nadal-Ginard, B. & Shows, T. Science 221, 766–769 (1983).

    ADS  CAS  Article  Google Scholar 

  17. 17

    Buckingham, M. in Essays in Biochemistry Vol. 20 (eds Campbell, P. N. & Marshall, R. D.)(Academic, New York, 1984).

    Google Scholar 

  18. 18

    Maniatis, T., Fritsch, E. F., Lauer, J. & Lawn, R. M. A. Rev. Genet. 14, 145–178 (1980).

    CAS  Article  Google Scholar 

  19. 19

    Bonhomme, F., Benmehdi, F., Britton-Davidian, J. & Martin, S. C.r. hebd. Séanc. Acad. Sci., Paris 289 D, 545–548 (1979).

    CAS  Google Scholar 

  20. 20

    Harris, H. & Hopkinson, D. A. Handbook of Enzyme Electrophoresis in Human Genetics (North-Holland, Amsterdam, 1978).

    Google Scholar 

  21. 21

    Rougeon, F. & Mach, B. Gene 1, 229–239 (1977).

    CAS  Article  Google Scholar 

  22. 22

    Bonhomme, F., Guénet, J-L. & Catalan, J. C.r. hebd. Séanc. Acad. Sci., Paris 294, 691–693 (1982).

    CAS  Google Scholar 

  23. 23

    Minty, A. J., Alonso, S., Guénet, J-L. & Buckingham, M. E. J. molec. Biol. 167, 77–101 (1983).

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Robert, B., Barton, P., Minty, A. et al. Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross. Nature 314, 181–183 (1985). https://doi.org/10.1038/314181a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing