Abstract
The introduction of cloned probes to follow the segregation of DNA restriction fragment length polymorphisms (RFLPs) has led to a revival of mendelian genetics in attempts to map the human genome1. In the mouse, however, it has often proved difficult to detect an RFLP with a DNA probe between different inbred strains of the laboratory mouse. To circumvent this problem, we have used two species, Mus musculus domesticus and Mus spretus2 which interact as sympatric species3 but can be interbred under laboratory conditions4. Because of the relative evolutionary distance between these species, they exhibit polymorphism at many more loci than do different strains of the usual M. m. domesticus laboratory mouse2. This is also observed at the DNA level when the sizes of restriction fragments encoding a specific gene are compared5. We have used these RFLPs between M. m. domesticus and M. spretus to follow the segregation of genes encoding different isoforms of myosin alkali light chains in the backcross progeny between these species and to compare this with that of other contractile protein genes. No linkage between these genes was observed.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Solomon, E. & Goodfellow, P. Nature 306, 223–224 (1983).
Bonhomme, F. et al. Biochem. Genet. 22, 275–303 (1984).
Britton-Davidian, J. & Thaler, L. Biochem. Genet. 16, 213–225 (1978).
Bonhomme, F., Martin, S. & Thaler, L. Experientia 34, 1140–1141 (1978).
Robert, B. et al. Cell 29, 129–140 (1984).
Barton, P. J. R. et al. J. biol. Chem. (submitted).
Whalen, R. G. & Sell, S. Nature 286, 731–733 (1980).
Barton, P. J. R., Robert, B., Fiszman, M. Y., Leader, D. P. & Buckingham, M. E. J. Muscle Res. Cell Motility (in the press).
Taylor, B. A. in Origins of Inbred Mice (ed. Morse, H.) 423–438 (Academic, New York, 1978).
Genetic Variants and Strains of the Laboratory Mouse (ed. Green, M.)(Fischer, Stuttgart, 1981).
Minty, A. J. et al. J. biol. Chem. 256 1008–1014 (1981).
Minty, A. J., Alonso, S., Caravatti, M. & Buckingham, M. E. Cell 30, 185–192 (1982).
Weydert, A. et al. J. biol. Chem. 258 13867–13874 (1983).
Czosnek, H. et al. EMBO J. 1, 1299–1305 (1982).
Czosnek, H. et al. EMBO J. 2, 1977–1979 (1983).
Leinwand, L. A., Fournier, R. E. K., Nadal-Ginard, B. & Shows, T. Science 221, 766–769 (1983).
Buckingham, M. in Essays in Biochemistry Vol. 20 (eds Campbell, P. N. & Marshall, R. D.)(Academic, New York, 1984).
Maniatis, T., Fritsch, E. F., Lauer, J. & Lawn, R. M. A. Rev. Genet. 14, 145–178 (1980).
Bonhomme, F., Benmehdi, F., Britton-Davidian, J. & Martin, S. C.r. hebd. Séanc. Acad. Sci., Paris 289 D, 545–548 (1979).
Harris, H. & Hopkinson, D. A. Handbook of Enzyme Electrophoresis in Human Genetics (North-Holland, Amsterdam, 1978).
Rougeon, F. & Mach, B. Gene 1, 229–239 (1977).
Bonhomme, F., Guénet, J-L. & Catalan, J. C.r. hebd. Séanc. Acad. Sci., Paris 294, 691–693 (1982).
Minty, A. J., Alonso, S., Guénet, J-L. & Buckingham, M. E. J. molec. Biol. 167, 77–101 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Robert, B., Barton, P., Minty, A. et al. Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross. Nature 314, 181–183 (1985). https://doi.org/10.1038/314181a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/314181a0
This article is cited by
-
Margaret Buckingham, discoveries in skeletal and cardiac muscle development, elected to the National Academy of Science
Skeletal Muscle (2012)
-
Msx1 is close but not allelic to either Hm or Hx on mouse Chromosome 5
Mammalian Genome (1994)
-
Evolution of EF-hand calcium-modulated proteins. V. The genes encoding EF-hand proteins are not clustered in mammalian genomes
Journal of Molecular Evolution (1993)
-
Re-localization of Actsk-1 to mouse Chromosome 8, a new region of homology with human Chromosome 1
Mammalian Genome (1993)
-
Mapping of multiple mouse loci related to the farnesyl pyrophosphate synthetase gene
Mammalian Genome (1993)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.