DNA of human papillomavirus (HPV) types 16 and 18 has been found closely associated with human genital cancer1,2, supporting the concept that members of this virus group are key factors in the aetiology of genital cancer3. HPV 18 DNA sequences were also detected in cell lines derived from cervical cancer2. We have now analysed these cell lines, HeLa, C4-1 and 756, for the structural organization and transcription of the HPV 18 genome and we find that the HPV 18 DNA is integrated into the cellular genome and is amplified in HeLa and 756 cells. Almost the complete HPV 18 genome seems to be present in 756 cells, with the early region being disrupted into two portions in each integrated copy. In HeLa and C4-1 cells, a 2–3 kilobase (kb) segment of HPV 18-specific sequences is missing from the E2 to L2 region. HPV 18 sequences are specifically transcribed from the E6–E7–E1 region into poly(A)+ RNAs of 1.5–6.5 kb. Hybridization analysis of cDNA clones indicated that some of the transcripts are composed of HPV 18 and cellular sequences. In addition, poly(A)+ RNA hybridizing with HPV 16 DNA was found in two out of three cervical carcinoma biopsies.
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Dürst, M., Gissmann, L., Ikenberg, H. & zur Hausen, H. Proc. natn. Acad. Sci. U.S.A. 80, 3812–3815 (1983).
Boshart, M. et al. EMBO J. 3, 1151–1157 (1984).
zur Hausen, H. Curr. Topics Microbiol. Immun. 78, 1–30 (1977).
Wettstein, F. O. & Stevens, J. Proc. natn. Acad. Sci. U.S.A. 79, 790–794 (1982).
Gissmann, L., de Villiers, E.-M. & zur Hausen, H. Int. J. Cancer 29, 143–146 (1982).
Clewell, D. B. & Helinski, D. R. Proc. natn. Acad. Sci. U.S.A. 62, 1159–1166 (1969).
Schwarz, E. et al. EMBO J. 2, 2341–2348 (1983).
Gissmann, L. & Schwarz, E. in Development in Molecular Virology Vol. 5 (ed. Becker, Y.) 173–197 (Nijhoff, Hingham, Massachusetts, 1985).
Heilman, C. A., Engel, L., Lowy, D. R. & Howley, P. M. Virology 119, 22–34 (1982).
Freese, U. K., Schulte, P. & Pfister, H. Virology 117, 257–261 (1982).
Amtmann, E. & Sauer, G. J. Virol. 43, 59–66 (1982).
Pfister, H. Rev. Physiol. Biochem. Pharm. 99, 111–181 (1984).
Georges, E., Croissant, O., Bonneaud, N. & Orth, G. J. Virol. 51, 530–538 (1984).
McVay, P., Fretz, M., Wettstein, F. O., Stevens, J. G. & Ito, Y. J. gen. Virol. 60, 271–278 (1982).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Auersperg, N. & Hawryluk, A. F. J. natn. Cancer Inst. 28, 605–627 (1962).
Freedman, R. S. et al. in vitro 18, 719–726 (1982).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
Freese, U. K., Laux, G., Hudewentz, J., Schwarz, E. & Bornkamm, G. W. J. Virol. 48, 731–743 (1983).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1413 (1972).
Seed, B. Nucleic Acids Res. 10, 1799–1810 (1982).
Moos, M. & Gallwitz, D. EMBO J. 2, 757–761 (1983).
Dürst, M., Kleinheinz, A., Hotz, M. & Gissmann, L. J. gen. Virol. (in the press).
About this article
Cite this article
Schwarz, E., Freese, U., Gissmann, L. et al. Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature 314, 111–114 (1985). https://doi.org/10.1038/314111a0
Cancer Letters (2020)
Microbial Pathogenesis (2020)
Development of multiepitope therapeutic vaccines against the most prevalent high-risk human papillomaviruses
Insertional oncogenesis by HPV70 revealed by multiple genomic analyses in a clinically HPV‐negative cervical cancer
Genes, Chromosomes and Cancer (2020)
Biochemical Pharmacology (2020)