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Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

Nature volume 313, pages 358363 (31 January 1985) | Download Citation

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Abstract

Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose l,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.

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Author information

Author notes

    • Michael P. Timko
    • , Albert P. Kausch
    •  & Anthony R. Cashmore

    Laboratory of Cell Biology, The Rockefeller University, New York, New York 10021, USA

Affiliations

  1. Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium

    • Guido Van den Broeck
    • , Michael P. Timko
    • , Albert P. Kausch
    • , Anthony R. Cashmore
    • , Marc Van Montagu
    •  & Luis Herrera-Estrella

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https://doi.org/10.1038/313358a0

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