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Cloning, sequence and expression of human interleukin-2 receptor

Nature volume 312, pages 768–771 (1984)Cite this article

Abstract

T lymphocytes, essential for the generation of a normal immune response, require the presence of the lymphokine interleukin-2 (IL-2) in order to proliferate1,2. Cells that respond to IL-2 possess a surface receptor glycoprotein specific for this lymphokine3,4. We have recently purified and chemically characterized the IL-2 receptor from both phytohaemagglutinin-activated human T cells and the human T-cell lymphoma HUT-102 (ref. 5). From the NH2-terminal protein sequence obtained in that study, we have now used synthetic oligonucleotides to probe a complementary DNA library, prepared from HUT-102 messenger RNA, for the presence of cDNA clones that might code for the IL-2 receptor. Two cDNA clones were isolated which had closely related DNA sequences. Interestingly, only one coded for an active receptor when transfected into COS-7 cells. This clone contained a 216-base pair (bp) insert that was not present in the other clone. The insert was flanked by an 8-bp direct repeat reminiscent of a transposable element, and appeared to code for a region of marked structural homology to the NH2-terminal region of the receptor molecule.

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Authors and Affiliations

  1. Department of Molecular Biology, Immunex Corporation, 51 University Street, Seattle, Washington, 98101, USA

    David Cosman, Douglas Pat Cerretti & Alf Larsen

  2. Department of Membrane Biochemistry, Immunex Corporation, 51 University Street, Seattle, Washington, 98101, USA

    Linda Park, Steven Dower, Steven Gillis & David Urdal

  3. Department of Protein Chemistry, Immunex Corporation, 51 University Street, Seattle, Washington, 98101, USA

    Carl March

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  1. David Cosman
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  2. Douglas Pat Cerretti
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Cosman, D., Pat Cerretti, D., Larsen, A. et al. Cloning, sequence and expression of human interleukin-2 receptor. Nature 312, 768–771 (1984). https://doi.org/10.1038/312768a0

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