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The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein

Abstract

To study the nature of antigenic recognition, antibodies have been prepared against a set of peptide sequences representing both highly mobile and well-ordered regions of myohaemerythrin, based on X-ray crystallographic temperature factors. Anti-peptide antibodies against highly mobile regions react strongly with the native protein; anti-peptide antibodies from well-ordered regions do not. Mobility is a major factor in the recognition of the native protein by anti-peptide antibodies; this may be of general significance in protein–protein interactions.

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Tainer, J., Getzoff, E., Alexander, H. et al. The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein. Nature 312, 127–134 (1984). https://doi.org/10.1038/312127a0

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