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The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein

Nature volume 312, pages 127–134 (1984)Cite this article

Abstract

To study the nature of antigenic recognition, antibodies have been prepared against a set of peptide sequences representing both highly mobile and well-ordered regions of myohaemerythrin, based on X-ray crystallographic temperature factors. Anti-peptide antibodies against highly mobile regions react strongly with the native protein; anti-peptide antibodies from well-ordered regions do not. Mobility is a major factor in the recognition of the native protein by anti-peptide antibodies; this may be of general significance in protein–protein interactions.

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References

  1. Lerner, R. A. Nature 299, 592–596 (1982).

    Article  ADS  CAS  Google Scholar 

  2. Crumpton, M. J. in The Antigens (ed. Sela, M.) 133–158 (Academic, New York, 1974).

    Google Scholar 

  3. Niman, H. L. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4949–4953 (1983).

    Article  ADS  CAS  Google Scholar 

  4. Wilson, I. A. et al. Cell 37, 767–778 (1984).

    Article  CAS  Google Scholar 

  5. Debye, P. Ann. Phys. 43, 49 (1914).

    CAS  Google Scholar 

  6. Holbrook, S. R. & Kim, S.-H. J. molec. Biol. 173, 361–388 (1984).

    Article  CAS  Google Scholar 

  7. Swaminathan, S., Ichiye, T., van Gunsteren, W. & Karplus, M. Biochemistry 21, 5230–5241 (1982).

    Article  CAS  Google Scholar 

  8. Artymiuk, P. J. et al. Nature 280, 563–568 (1979).

    Article  ADS  CAS  Google Scholar 

  9. Bernstein, F. C. et al. J. molec. Biol. 112, 535–542 (1977).

    Article  CAS  Google Scholar 

  10. Richards, F. M. A. Rev. Biophys. Bioengng 6, 151–176 (1977).

    Article  CAS  Google Scholar 

  11. Weber, P. C. & Salemme, F. R. Nature 287, 82–84 (1980).

    Article  ADS  CAS  Google Scholar 

  12. Stenkamp, R. E., Sieker, L. C. & Jensen, L. H. Acta crystallogr. B 39, 697–703 (1983).

    Article  Google Scholar 

  13. Frauenfelder, H. & Petsko, G. A. Biophys. J. 32, 465–483 (1980).

    Article  ADS  CAS  Google Scholar 

  14. Levitt, M. J. molec. Biol. 168, 621–657 (1983).

    Article  CAS  Google Scholar 

  15. Farr, R. S. J. infect. Dis. 103, 239–262 (1958).

    Article  CAS  Google Scholar 

  16. Klippenstein, G. L., Van Riper, D. A. & Oosterom, E. A. J. biol. Chem. 247, 5959–5963 (1972).

    CAS  PubMed  Google Scholar 

  17. McCammon, J. A. & Karplus, M. Acc. chem. Res. 16, 187–193 (1983).

    Article  CAS  Google Scholar 

  18. Huber, R. & Bennett, W. S. Jr Biopolymers 22, 261–279 (1983).

    Article  CAS  Google Scholar 

  19. Mao, B., Pear, M. R., McCammon, J. A. & Northrup, S. H. Biopolymers 21, 1979–1989 (1982).

    Article  CAS  Google Scholar 

  20. Wagner, G. & Wuthrich, K. J. molec. Biol. 134, 75–94 (1979).

    Article  CAS  Google Scholar 

  21. Benjamin, D. C. et al. A. Rev. Immun. 2, 67–101 (1984).

    Article  CAS  Google Scholar 

  22. Chothia, C. & Janin, J. Nature 256, 705–708 (1975).

    Article  ADS  CAS  Google Scholar 

  23. Getzoff, E. D. et al. Nature 306, 287–290 (1983).

    Article  ADS  CAS  Google Scholar 

  24. Weiner, P. K., Langridge, R., Blaney, J. M., Schaefer, R. & Kollman, P. A. Proc. natn. Acad. Sci. U.S.A. 79, 3754–3758 (1982).

    Article  ADS  CAS  Google Scholar 

  25. Connolly, M. L. Science 221, 709–713 (1983).

    Article  ADS  CAS  Google Scholar 

  26. Eisenberg, D., Weiss, R. M., Terwilliger, T. C. & Wilcox, W. Faraday Symp. chem. Soc. 17, 109–120 (1982).

    Article  Google Scholar 

  27. Green, N. et al. Cell 28, 477–487 (1982).

    Article  CAS  Google Scholar 

  28. Johnson, D. A., Gautsch, J. W., Sportsman, J. R. & Elder, J. H. Gene analyt. Tech. 1, 3–8 (1984).

    Article  CAS  Google Scholar 

  29. Ternynck, T. & Avranmeas, S. Immunochemistry 14, 767–774 (1977).

    Article  CAS  Google Scholar 

  30. McConahey, P. J. & Dixon, F. J. Int. Archs Allergy appl. Immun. 29, 185–189 (1966).

    Article  CAS  Google Scholar 

  31. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).

    Article  ADS  CAS  Google Scholar 

  32. Connolly, M. L. J. Appl. Crystallogr. 16, 548–558 (1983).

    Article  CAS  Google Scholar 

  33. McCammon, J. A., Wolynes, P. & Karplus, M. Biochemistry 18, 927–942 (1979).

    Article  CAS  Google Scholar 

  34. Bondi, A. J. phys. Chem. 68, 441–451 (1964).

    Article  CAS  Google Scholar 

  35. O'Donnell, T. J. & Olson, A. J. Comput. Graphics 15, 133–142 (1981).

    Article  Google Scholar 

  36. Connolly, M. L. & Olson, A. J. Computers and Chemistry (in the press).

  37. Connolly, M. L. J. molec. Graphics (in the press).

  38. Klippenstein, G. L., Cote, J. L. & Ludlan, S. E. Biochemistry 15, 1128–1136 (1976).

    Article  CAS  Google Scholar 

  39. Houghten, R. A., Chang, W. C. & Li, C. H. Int. J. Peptide Prot. Res. 16, 311–320 (1980).

    Article  CAS  Google Scholar 

  40. Marglin, A. & Merrifield, R. B. A. Rev. Biochem. 39, 841–866 (1970).

    Article  CAS  Google Scholar 

  41. Bittle, J. L. et al. Nature 298, 30–33 (1982).

    Article  ADS  CAS  Google Scholar 

  42. Houghten, R. A. & Li, C. H. Analyt. Biochem. 98, 36–46 (1979).

    Article  CAS  Google Scholar 

  43. Liu, F. T., Zinnecker, M., Hamaska, T. & Katz, D. H. Biochemistry 18, 690–697 (1979).

    Article  CAS  Google Scholar 

  44. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  45. Sheriff, S., Hendrickson, W. A., Stenkamp, R. E., Sieker, L. C. & Jensen, L. H. Proc. natn. Acad. Sci. U.S.A. (in the press).

  46. Westhof, E. et al. Nature 311, 123–126 (1984).

    Article  ADS  CAS  Google Scholar 

  47. Tainer, J. A., Getzoff, E. D., Paterson, Y., Olson, A. J. & Lerner, R. A. A. Rev. Immun. (in the press).

Download references

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Authors and Affiliations

  1. Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California, 92037, USA

    John A. Tainer, Elizabeth D. Getzoff, Hannah Alexander, Richard A. Houghten, Arthur J. Olson & Richard A. Lerner

  2. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, 10032, USA

    Wayne A. Hendrickson

Authors
  1. John A. Tainer
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  2. Elizabeth D. Getzoff
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  3. Hannah Alexander
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  4. Richard A. Houghten
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  5. Arthur J. Olson
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  6. Richard A. Lerner
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  7. Wayne A. Hendrickson
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Tainer, J., Getzoff, E., Alexander, H. et al. The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein. Nature 312, 127–134 (1984). https://doi.org/10.1038/312127a0

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