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The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein

Nature volume 312, pages127–134(1984)Cite this article

Abstract

To study the nature of antigenic recognition, antibodies have been prepared against a set of peptide sequences representing both highly mobile and well-ordered regions of myohaemerythrin, based on X-ray crystallographic temperature factors. Anti-peptide antibodies against highly mobile regions react strongly with the native protein; anti-peptide antibodies from well-ordered regions do not. Mobility is a major factor in the recognition of the native protein by anti-peptide antibodies; this may be of general significance in protein–protein interactions.

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References

  1. 1

    Lerner, R. A. Nature 299, 592–596 (1982).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Crumpton, M. J. in The Antigens (ed. Sela, M.) 133–158 (Academic, New York, 1974).

    Google Scholar 

  3. 3

    Niman, H. L. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4949–4953 (1983).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Wilson, I. A. et al. Cell 37, 767–778 (1984).

    CAS  Article  Google Scholar 

  5. 5

    Debye, P. Ann. Phys. 43, 49 (1914).

    CAS  Google Scholar 

  6. 6

    Holbrook, S. R. & Kim, S.-H. J. molec. Biol. 173, 361–388 (1984).

    CAS  Article  Google Scholar 

  7. 7

    Swaminathan, S., Ichiye, T., van Gunsteren, W. & Karplus, M. Biochemistry 21, 5230–5241 (1982).

    CAS  Article  Google Scholar 

  8. 8

    Artymiuk, P. J. et al. Nature 280, 563–568 (1979).

    ADS  CAS  Article  Google Scholar 

  9. 9

    Bernstein, F. C. et al. J. molec. Biol. 112, 535–542 (1977).

    CAS  Article  Google Scholar 

  10. 10

    Richards, F. M. A. Rev. Biophys. Bioengng 6, 151–176 (1977).

    CAS  Article  Google Scholar 

  11. 11

    Weber, P. C. & Salemme, F. R. Nature 287, 82–84 (1980).

    ADS  CAS  Article  Google Scholar 

  12. 12

    Stenkamp, R. E., Sieker, L. C. & Jensen, L. H. Acta crystallogr. B 39, 697–703 (1983).

    Article  Google Scholar 

  13. 13

    Frauenfelder, H. & Petsko, G. A. Biophys. J. 32, 465–483 (1980).

    ADS  CAS  Article  Google Scholar 

  14. 14

    Levitt, M. J. molec. Biol. 168, 621–657 (1983).

    CAS  Article  Google Scholar 

  15. 15

    Farr, R. S. J. infect. Dis. 103, 239–262 (1958).

    CAS  Article  Google Scholar 

  16. 16

    Klippenstein, G. L., Van Riper, D. A. & Oosterom, E. A. J. biol. Chem. 247, 5959–5963 (1972).

    CAS  PubMed  Google Scholar 

  17. 17

    McCammon, J. A. & Karplus, M. Acc. chem. Res. 16, 187–193 (1983).

    CAS  Article  Google Scholar 

  18. 18

    Huber, R. & Bennett, W. S. Jr Biopolymers 22, 261–279 (1983).

    CAS  Article  Google Scholar 

  19. 19

    Mao, B., Pear, M. R., McCammon, J. A. & Northrup, S. H. Biopolymers 21, 1979–1989 (1982).

    CAS  Article  Google Scholar 

  20. 20

    Wagner, G. & Wuthrich, K. J. molec. Biol. 134, 75–94 (1979).

    CAS  Article  Google Scholar 

  21. 21

    Benjamin, D. C. et al. A. Rev. Immun. 2, 67–101 (1984).

    CAS  Article  Google Scholar 

  22. 22

    Chothia, C. & Janin, J. Nature 256, 705–708 (1975).

    ADS  CAS  Article  Google Scholar 

  23. 23

    Getzoff, E. D. et al. Nature 306, 287–290 (1983).

    ADS  CAS  Article  Google Scholar 

  24. 24

    Weiner, P. K., Langridge, R., Blaney, J. M., Schaefer, R. & Kollman, P. A. Proc. natn. Acad. Sci. U.S.A. 79, 3754–3758 (1982).

    ADS  CAS  Article  Google Scholar 

  25. 25

    Connolly, M. L. Science 221, 709–713 (1983).

    ADS  CAS  Article  Google Scholar 

  26. 26

    Eisenberg, D., Weiss, R. M., Terwilliger, T. C. & Wilcox, W. Faraday Symp. chem. Soc. 17, 109–120 (1982).

    Article  Google Scholar 

  27. 27

    Green, N. et al. Cell 28, 477–487 (1982).

    CAS  Article  Google Scholar 

  28. 28

    Johnson, D. A., Gautsch, J. W., Sportsman, J. R. & Elder, J. H. Gene analyt. Tech. 1, 3–8 (1984).

    CAS  Article  Google Scholar 

  29. 29

    Ternynck, T. & Avranmeas, S. Immunochemistry 14, 767–774 (1977).

    CAS  Article  Google Scholar 

  30. 30

    McConahey, P. J. & Dixon, F. J. Int. Archs Allergy appl. Immun. 29, 185–189 (1966).

    CAS  Article  Google Scholar 

  31. 31

    Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).

    ADS  CAS  Article  Google Scholar 

  32. 32

    Connolly, M. L. J. Appl. Crystallogr. 16, 548–558 (1983).

    CAS  Article  Google Scholar 

  33. 33

    McCammon, J. A., Wolynes, P. & Karplus, M. Biochemistry 18, 927–942 (1979).

    CAS  Article  Google Scholar 

  34. 34

    Bondi, A. J. phys. Chem. 68, 441–451 (1964).

    CAS  Article  Google Scholar 

  35. 35

    O'Donnell, T. J. & Olson, A. J. Comput. Graphics 15, 133–142 (1981).

    Article  Google Scholar 

  36. 36

    Connolly, M. L. & Olson, A. J. Computers and Chemistry (in the press).

  37. 37

    Connolly, M. L. J. molec. Graphics (in the press).

  38. 38

    Klippenstein, G. L., Cote, J. L. & Ludlan, S. E. Biochemistry 15, 1128–1136 (1976).

    CAS  Article  Google Scholar 

  39. 39

    Houghten, R. A., Chang, W. C. & Li, C. H. Int. J. Peptide Prot. Res. 16, 311–320 (1980).

    CAS  Article  Google Scholar 

  40. 40

    Marglin, A. & Merrifield, R. B. A. Rev. Biochem. 39, 841–866 (1970).

    CAS  Article  Google Scholar 

  41. 41

    Bittle, J. L. et al. Nature 298, 30–33 (1982).

    ADS  CAS  Article  Google Scholar 

  42. 42

    Houghten, R. A. & Li, C. H. Analyt. Biochem. 98, 36–46 (1979).

    CAS  Article  Google Scholar 

  43. 43

    Liu, F. T., Zinnecker, M., Hamaska, T. & Katz, D. H. Biochemistry 18, 690–697 (1979).

    CAS  Article  Google Scholar 

  44. 44

    Laemmli, U. K. Nature 227, 680–685 (1970).

    ADS  CAS  Article  Google Scholar 

  45. 45

    Sheriff, S., Hendrickson, W. A., Stenkamp, R. E., Sieker, L. C. & Jensen, L. H. Proc. natn. Acad. Sci. U.S.A. (in the press).

  46. 46

    Westhof, E. et al. Nature 311, 123–126 (1984).

    ADS  CAS  Article  Google Scholar 

  47. 47

    Tainer, J. A., Getzoff, E. D., Paterson, Y., Olson, A. J. & Lerner, R. A. A. Rev. Immun. (in the press).

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Author information

Affiliations

  1. Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California, 92037, USA

    John A. Tainer, Elizabeth D. Getzoff, Hannah Alexander, Richard A. Houghten, Arthur J. Olson & Richard A. Lerner

  2. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, 10032, USA

    Wayne A. Hendrickson

Authors
  1. John A. Tainer
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  2. Elizabeth D. Getzoff
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  3. Hannah Alexander
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  4. Richard A. Houghten
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  5. Arthur J. Olson
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  6. Richard A. Lerner
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  7. Wayne A. Hendrickson
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Tainer, J., Getzoff, E., Alexander, H. et al. The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein. Nature 312, 127–134 (1984). https://doi.org/10.1038/312127a0

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