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Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence

Nature volume 312, pages 121–127 (1984)Cite this article

Abstract

Cloning and sequence analysis of cDNA for the Electrophorus electricus electroplax sodium channel indicate that this protein, consisting of 1,820 amino acid residues, exhibits four repeated homology units, which are presumably oriented in a pseudosymmetric fashion across the membrane. Each homology unit contains a unique segment with clustered positively charged residues, which may be involved in the gating structure, possibly in conjunction with negatively charged residues clustered elsewhere.

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References

  1. Agnew, W. S. A. Rev. Physiol. 46, 517–530 (1984).

    Article  CAS  Google Scholar 

  2. Catterall, W. A. Science 223, 653–661 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Hodgkin, A. L. & Huxley, A. F. J. Physiol., Lond. 117, 500–544 (1952).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Catterall, W. A. A. Rev. Pharmac. Tox. 20, 15–43 (1980).

    Article  CAS  Google Scholar 

  5. Agnew, W. S., Levinson, S. R., Brabson, J. S. & Raftery, M. A. Proc. natn. Acad. Sci. U.S.A. 75, 2606–2610 (1978).

    Article  ADS  CAS  Google Scholar 

  6. Nakayama, H., Withy, R. M. & Raftery, M. A. Proc. natn. Acad. Sci. U.S.A. 79, 7575–7579 (1982).

    Article  ADS  CAS  Google Scholar 

  7. Miller, J. A., Agnew, W. S. & Levinson, S. R. Biochemistry 22, 462–470 (1983).

    Article  CAS  PubMed  Google Scholar 

  8. Hartshorne, R. P. & Catterall, W. A. Proc. natn. Acad. Sci. U.S.A. 78, 4620–4624 (1981).

    Article  ADS  CAS  Google Scholar 

  9. Barchi, R. L. J. Neurochem. 40, 1377–1385 (1983).

    Article  CAS  PubMed  Google Scholar 

  10. Lombet, A. & Lazdunski, M. Eur. J. Biochem. 141, 651–660 (1984).

    Article  CAS  PubMed  Google Scholar 

  11. Weigele, J. B. & Barchi, R. L. Proc. natn. Acad. Sci. U.S.A. 79, 3651–3655 (1982).

    Article  ADS  CAS  Google Scholar 

  12. Tamkun, M. M., Talvenheimo, J. A. & Catterall, W. A. J. biol. Chem. 259, 1676–1688 (1984).

    CAS  PubMed  Google Scholar 

  13. Rosenberg, R. L., Tomiko, S. A. & Agnew, W. S. Proc. natn. Acad. Sci. U.S.A. 81, 1239–1243 (1984).

    Article  ADS  CAS  Google Scholar 

  14. Hanke, W., Boheim, G., Barhanin, J., Pauron, D. & Lazdunski, M. EMBO J. 3, 509–515 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Hewick, R. M., Hunkapiller, M. W., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990–7997 (1981).

    CAS  PubMed  Google Scholar 

  16. Vieira, J. & Messing, J. Gene 19, 259–268 (1982).

    Article  CAS  PubMed  Google Scholar 

  17. Helfman, D. M., Feramisco, J. R., Fiddes, J. C., Thomas, G. P. & Hughes, S. H. Proc. natn. Acad. Sci. U.S.A. 80, 31–35 (1983).

    Article  ADS  CAS  Google Scholar 

  18. Southern, E. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  19. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  PubMed  Google Scholar 

  20. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  21. Okayama, H. & Berg, P. Molec. cell. Biol. 2, 161–170 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Hanahan, D. & Meselson, M. Gene 10, 63–67 (1980).

    Article  CAS  PubMed  Google Scholar 

  23. Kozak, M. Nucleic Acids Res. 9, 5233–5252 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Hubbard, S. C. & Ivatt, R. J. A. Rev. Biochem. 50, 555–583 (1981).

    Article  CAS  Google Scholar 

  26. Bause, E. Biochem. J. 209, 331–336 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Toh, H., Hayashida, H. & Miyata, T. Nature 305, 827–829 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  28. Chou, P. Y. & Fasman, G. D. A. Rev. Biochem. 47, 251–276 (1978).

    Article  CAS  Google Scholar 

  29. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  PubMed  Google Scholar 

  30. Capaldi, R. A. & Vanderkooi, G. Proc. natn. Acad. Sci. U.S.A. 69, 930–932 (1972).

    Article  ADS  CAS  Google Scholar 

  31. Engelman, D. M. & Steitz, T. A. Cell 23, 411–422 (1981).

    Article  CAS  PubMed  Google Scholar 

  32. Rice, C. M., Bell, J. R., Hunkapiller, M. W., Strauss, E. G. & Strauss, J. H. J. molec. Biol. 154, 355–378 (1982).

    Article  CAS  PubMed  Google Scholar 

  33. Hille, B. J. gen. Physiol. 58, 599–619 (1971).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Hille, B. J. gen. Physiol. 59, 637–658 (1972).

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  35. Hille, B. in Membranes: A Series of Advances Vol. 3 (ed. Eisenman, G.) 255–323 (Dekker, New York, 1975).

    Google Scholar 

  36. Kreil, G. A. Rev. Biochem. 50, 317–348 (1981).

    Article  CAS  Google Scholar 

  37. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 852–862 (1975).

    Article  CAS  PubMed  Google Scholar 

  38. Braell, W. A. & Lodish, H. F. Cell 28, 23–31 (1982).

    Article  CAS  PubMed  Google Scholar 

  39. Anderson, D. J., Mostov, K. E. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 80, 7249–7253 (1983).

    Article  ADS  CAS  Google Scholar 

  40. Claesson, L., Larhammar, D., Rask, L. & Paterson, P. A. Proc. natn. Acad. Sci. U.S.A. 80, 7395–7399 (1983).

    Article  ADS  CAS  Google Scholar 

  41. Chin, D. J. et al. Nature 308, 613–617 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  42. Armstrong, C. M. & Bezanilla, F. Nature 242, 459–461 (1973).

    Article  ADS  CAS  PubMed  Google Scholar 

  43. Armstrong, C. M. Q. Rev. Biophys. 7, 179–210 (1975).

    Article  Google Scholar 

  44. Armstrong, C. M. Physiol. Rev. 61, 644–683 (1981).

    Article  CAS  PubMed  Google Scholar 

  45. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  46. Sutcliffe, J. G. Nucleic Acids Res. 5, 2721–2728 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Reddy, V. B. et al. Science 200, 494–502 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  48. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  PubMed  Google Scholar 

  49. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  50. Hanahan, D. J. molec. Biol. 166, 557–580 (1983).

    Article  CAS  PubMed  Google Scholar 

  51. Moore, H.-P. H., Fritz, L. C., Raftery, M. A. & Brockes, J. P. Proc. natn. Acad. Sci. U.S.A. 79, 1673–1677 (1982).

    Article  ADS  CAS  Google Scholar 

  52. Ito, H., Ike, Y., Ikuta, S. & Itakura, K. Nucleic Acids Res. 10, 1755–1769 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Boyer, H. W. & Roulland-Dussoix, D. J. molec. Biol. 41, 459–472 (1969).

    Article  CAS  PubMed  Google Scholar 

  54. Wahl, G. M., Padgett, R. A. & Stark, G. R. J. biol. Chem. 254, 8679–8689 (1979).

    CAS  PubMed  Google Scholar 

  55. Weinstock, R., Sweet, R., Weiss, M., Cedar, H. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 75, 1299–1303 (1978).

    Article  ADS  CAS  Google Scholar 

  56. Noda, M. et al. Nature 295, 202–206 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  57. McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).

    Article  ADS  CAS  Google Scholar 

  58. Dayhoff, M. O., Schwartz, R. M. & Orcutt, B. C. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 345–352 (National Biomedical Research Foundation, Silver Spring, Maryland, 1978).

    Google Scholar 

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Author information

Author notes

  1. Hitoshi Nakayama and Yuichi Kanaoka: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

  2. Naoto Minamino, Kenji Kangawa and Hisayuki Matsuo: Department of Biochemistry, Miyazaki Medical College, Miyazaki 889-16, Japan

  3. Michael A. Raftery: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA

  4. Tadaaki Hirose and Seiichi Inayama: Pharmaceutical Institute, Keio University School of Medicine, Tokyo 160, Japan

  5. Hidenori Hayashida and Takashi Miyata: Department of Biology, Kyushu University Faculty of Science, Fukuoka 812, Japan

Authors and Affiliations

  1. Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Masaharu Noda, Shin Shimizu, Tsutomu Tanabe, Toshiyuki Takai, Toshiaki Kayano, Takayuki Ikeda, Hideo Takahashi, Hitoshi Nakayama, Yuichi Kanaoka, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Michael A. Raftery, Tadaaki Hirose, Seiichi Inayama, Hidenori Hayashida, Takashi Miyata & Shosaku Numa  

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Noda, M., Shimizu, S., Tanabe, T. et al. Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence. Nature 312, 121–127 (1984). https://doi.org/10.1038/312121a0

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