Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Structure of the nucleosome core particle at 7 Å resolution

Nature volume 311, pages 532–537 (1984)Cite this article

Abstract

The crystal structure of the nucleosome core particle has been solved to 7 Å resolution. The right-handed B-DNA superhelix on the outside contains several sharp bends and makes numerous interactions with the histone octamer within. The central turn of superhelix and H3 · H4 tetramer have dyad symmetry, but the H2A · H2B dimers show departures due to interparticle associations.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

References

  1. Kornberg, R. D. A. Rev. Biochem. 46, 931–954 (1977).

    Article  CAS  Google Scholar 

  2. McGhee, J. D. & Felsenfeld, G. A. Rev. Biochem. 49, 1115–1156 (1980).

    CAS  Google Scholar 

  3. Klug, A. Symposium on Nucleic Acid Res. (eds Mizobuchi, K., Watanabe, I. & Watson, J. D.) 91–112 (Academic, New York, 1983).

    Google Scholar 

  4. Noll, M. & Kornberg, R. D. J. molec. Biol. 109, 393–404 (1977).

    Article  CAS  Google Scholar 

  5. Finch, J. T. et al. Nature 269, 29–36 (1977).

    Article  ADS  CAS  Google Scholar 

  6. Finch, J. T. et al. J. molec. Biol. 145, 757–769 (1981).

    Article  CAS  Google Scholar 

  7. Finch, J. T., Lewit-Bentley, A., Bentley, G. A., Roth, M. & Timmins, P. A. Phil Trans. R. Soc. B290, 635–638 (1980).

    Article  CAS  Google Scholar 

  8. Bentley, G. A., Finch, J. T. & Lewit-Bentley, A. J. molec. Biol. 145, 771–784 (1981).

    Article  CAS  Google Scholar 

  9. Klug, A., Rhodes, D., Smith, J., Finch, J. T. & Thomas, J. O. Nature 287, 509–516 (1980).

    Article  ADS  CAS  Google Scholar 

  10. Mirzabekov, A. D., Shick, V. V., Belyavsky, A. V. & Bavykin, S. G. Proc. natn. Acad. Sci. U.S.A. 75, 4184–4188 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Shick, V. V., Belyavsky, A. G., Bavykin, S. G. & Mirazebekov, A. D. J. molec. Biol. 139, 491–517 (1980).

    Article  CAS  Google Scholar 

  12. Thomas, J. O. & Kornberg, R. D. FEBS Lett. 58, 353–358 (1975).

    Article  CAS  Google Scholar 

  13. Kornberg, R. D. & Thomas, J. O. Science 184, 865–868 (1974).

    Article  ADS  CAS  Google Scholar 

  14. Eickbush, T. H. & Moudrianakis, E. N. Biochemistry 17, 4955–4964.

  15. Lattman, E., Burlingame, R., Hatch, C. & Moundrianakis, E. N. Science 216, 1016–1018 (1982).

    Article  ADS  CAS  Google Scholar 

  16. Burlingame, R. & Moundrianakis, E. N. Science 216, 1016–1018 (1984).

    Google Scholar 

  17. Richmond, T. J., Finch, J. T. & Klug, A. Cold Spring Harb. Symp. quant. Biol. 47, 493–501 (1983).

    Article  Google Scholar 

  18. Lee, C-H., Misuzawa, H. & Kakefuda, T. Proc. natn. Acad. Sci. U.S.A. 78, 2838–2842 (1981).

    Article  ADS  CAS  Google Scholar 

  19. Lipka, J. J., Lippard, S. J. & Wall, J. S. Science 206, 1419–1421 (1979).

    Article  ADS  CAS  Google Scholar 

  20. Dodson, E. J. in Crystallographic Computing Techniques (ed. Ahmed, F. R.) 259–268 (Munksgaard, Copenhagen, 1976).

    Google Scholar 

  21. Cary, P. D., Moss, T. & Bradbury, E. M. Eur. J. Biochem. 89, 475–482 (1979).

    Article  Google Scholar 

  22. Lutter, L. C. J. molec. Biol. 117, 53–69 (1977).

    Article  CAS  Google Scholar 

  23. Sollner Webb, B. & Felsenfeld, G. Cell 10, 537–547 (1977).

    Article  CAS  Google Scholar 

  24. Lutter, L. C. J. molec. Biol. 124, 391–420 (1978).

    Article  CAS  Google Scholar 

  25. Lutter, L. C. Nucleic Acids Res. 6, 41–56 (1979).

    Article  ADS  CAS  Google Scholar 

  26. Crick, F. C. & Klug, A. Nature 255, 530–533 (1975).

    Article  ADS  CAS  Google Scholar 

  27. McGhee, J. D. & Felsenfeld, G. Proc. natn. Acad. Sci. U.S.A. 76, 2133–2137 (1979).

    Article  ADS  CAS  Google Scholar 

  28. Lomonossoff, G. P., Butler, P. J. G. & Klug, A. J. molec. Biol. 149, 745–760 (1981).

    Article  CAS  Google Scholar 

  29. Dickerson, R. E. & Drew, H. R. J. molec. Biol.. 125, 535–543 (1981).

    Google Scholar 

  30. Camerini-Otero, R. D. & Felsenfeld, G. Proc. natn. Acad. Sci. U.S.A. 74, 5519–5523 (1977).

    Article  ADS  CAS  Google Scholar 

  31. McGhee, J. D. & Felsenfeld, G. Nucleic Acids Res. 8, 2751–2769 (1980).

    Article  CAS  Google Scholar 

  32. McGhee, J. D., Nichol, J. M., Felsenfeld, G. & Rau, D. C. Cell 33, 831–841 (1982).

    Article  Google Scholar 

  33. Thoma, F., Koller, Th. & Klug, A. J. Cell Biol. 83, 403–427 (1979).

    Article  CAS  Google Scholar 

  34. Simpson, R. T. Biochemistry 17, 5524–5531 (1978).

    Article  CAS  Google Scholar 

  35. Allan, J., Hartman, P. G., Crane-Robinson, C. & Aviles, F. X. Nature 288, 675–679 (1980).

    Article  ADS  CAS  Google Scholar 

  36. Boulikas, T., Wiseman, J. M. & Garrard, W. T. Proc. natn. Acad. Sci. U.S.A. 77, 127–131 (1980).

    Article  ADS  CAS  Google Scholar 

  37. Thomas, J. O. & Khabaza, A. J. A. Eur. J. Biochem. 112, 501–511 (1980).

    Article  CAS  Google Scholar 

  38. Camerini-Otero, R. D., Sollener-Webb, B. & Felsenfeld, G. Cell 8, 333–347 (1976).

    Article  CAS  Google Scholar 

  39. Stockley, P. G. & Thomas, J. O. FEBS Lett. 99, 129–135 (1979).

    Article  CAS  Google Scholar 

  40. Thomas, J. O. & Oudet, P. Nucleic Acids Res. 71, 611–623 (1979).

    Article  Google Scholar 

  41. Simon, R. H., Camerini-Otero, R. D. & Felsenfeld, Nucleic Acids Res. 5, 4805–4818 (1978).

    Article  CAS  Google Scholar 

  42. Anderson, W. F., Ohlendorf, D. H., Takeda, Y. & Matthews, B. W. Nature 290, 754–758 (1981).

    Article  ADS  CAS  Google Scholar 

  43. McKay, D. B. & Steitz, T. A. Nature 290, 744–749 (1981).

    Article  ADS  CAS  Google Scholar 

  44. Pabo, C. O. & Lewis, M. Nature 298, 443–447 (1982).

    Article  ADS  CAS  Google Scholar 

  45. Frederick, C. A. et al. Nature 309, 327–331 (1984).

    Article  ADS  CAS  Google Scholar 

  46. Chao, M. W., Gralla, J. & Martinson, H. G. Biochemistry 18, 1068–1074 (1979).

    Article  CAS  Google Scholar 

  47. Simpson, T. R. & Stafford, D. W. Proc. natn. Acad. Sci. U.S.A. 80, 51–55 (1983).

    Article  ADS  CAS  Google Scholar 

  48. Zachau, H. G. & Igo-Kemenes, T. Cell 24, 597–598 (1981).

    Article  CAS  Google Scholar 

  49. Kornberg, R. D. Nature 292, 579–580 (1981).

    Article  ADS  CAS  Google Scholar 

  50. Kunkel, G. R. & Martinson, H. G. Nucleic Acids Res. 9, 6869–6888 (1981).

    Article  CAS  Google Scholar 

  51. Prunell, A. EMBO J. 1, 173–179 (1982).

  52. Ness, P. J., Labhart, P., Banz, E., Koller, Th. & Parish, R. W. J. molec. Biol. 166, 361–381 (1983).

    Article  CAS  Google Scholar 

  53. Andersson, K., Mahr, R., Bjorkroth, B. & Daneholt, B. Chromosoma 87, 33–48 (1982).

    Article  CAS  Google Scholar 

  54. Prior, C. P., Cantor, C. R., Johnson, E. M., Littau, V. C. & Allfrey, V. G. Cell 34, 1033–1042 (1983).

    Article  CAS  Google Scholar 

  55. Baer, B. & Rhodes, D. Nature 301, 482–488 (1983).

    Article  ADS  CAS  Google Scholar 

  56. Hayes, J. G. & Halliday, J. J. Instn Maths. Appl. 14, 89–103 (1974).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge, CB2 2QH, UK

    T. J. Richmond, J. T. Finch, B. Rushton, D. Rhodes & A. Klug

Authors
  1. T. J. Richmond
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. T. Finch
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Rushton
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Rhodes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Klug
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Richmond, T., Finch, J., Rushton, B. et al. Structure of the nucleosome core particle at 7 Å resolution. Nature 311, 532–537 (1984). https://doi.org/10.1038/311532a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/311532a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing