Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

A role for clathrin light chains in the recognition of clathrin cages by ‘uncoating ATPase’

Nature volume 311, pages 228–231 (1984)Cite this article

Abstract

A cycle of clathrin assembly and disassembly drives the formation of coated vesicles, intermediates in intracellular protein transport. The heavy chain of clathrin is needed for assembly, but the function of the clathrin light chains has remained obscure. An enzyme has now been purified which uses ATP hydrolysis to power the release of clathrin from coated vesicles, presumably recycling the coat protein for repeated rounds of vesicle budding. This ‘uncoating ATPase’ requires clathrin light chains for its action.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Goldstein, J. L., Anderson, R. G. W. & Brown, M. S. Nature 279, 679–685 (1979).

    Article  ADS  CAS  Google Scholar 

  2. Pearse, B. M. F. & Bretscher, M. S. A. Rev. Biochem. 50, 85–101 (1981).

    Article  CAS  Google Scholar 

  3. Pearse, B. M. F. Trends biochem. Sci. 5, 131–134 (1980).

    Article  CAS  Google Scholar 

  4. Ungewickell, E. & Branton, D. Nature 289, 420–422 (1981).

    Article  ADS  CAS  Google Scholar 

  5. Kirchhausen, T. & Harrison, S. C. Cell 23, 755–761 (1981).

    Article  CAS  Google Scholar 

  6. Pearse, B. M. F. Proc. natn. Acad. Sci. U.S.A. 73, 1255–1259 (1976).

    Article  ADS  CAS  Google Scholar 

  7. Woodward, M. P. & Roth, T. F. Proc. natn. Acad. Sci. U.S. A. 75, 4394–4398 (1978).

    Article  ADS  CAS  Google Scholar 

  8. Keen, J. H., Willingham, M. C. & Pastan, I. H. Cell 16, 303–312 (1979).

    Article  CAS  Google Scholar 

  9. Pearse, B. M. F. J. molec. Biol 126, 803–812 (1978).

    Article  CAS  Google Scholar 

  10. Brodsky, F. M. & Parham, P. J. molec. Biol 167, 197–204 (1983).

    Article  CAS  Google Scholar 

  11. Ungewickell, E. EMBO J. 2, 1401–1408 (1983).

    Article  CAS  Google Scholar 

  12. Winkler, F. K. & Stanley, K. K. EMBO J. 2, 1393–1400 (1983).

    Article  CAS  Google Scholar 

  13. Kirchhausen, T., Harrison, S. C., Parham, P. & Brodsky, F. M. Proc. natn. Acad. Sci. U.S.A. 80, 2481–2485 (1983).

    Article  ADS  CAS  Google Scholar 

  14. Rothman, J. E., Fries, E., Dunphy, W. C. & Urbani, L. J. Cold Spring Harb. Symp. quant. Biol. 46, 797–805 (1982).

    Article  Google Scholar 

  15. Patzer, E. J., Schlossman, D. M. & Rothman, J. E. J. Cell Biol. 93, 230–236 (1982).

    Article  CAS  Google Scholar 

  16. Schlossman, D. M., Schmid, S. L., Braell, W. A. & Rothman, J. E. J. Cell Biol. 99, 723–733 (1984).

    Article  CAS  Google Scholar 

  17. Braell, W. A., Schlossman, D. M., Schmid, S. L. & Rothman, J. E. J. Cell Biol. 99, 734–741 (1984).

    Article  CAS  Google Scholar 

  18. Lisanti, M. P. et al. Eur. J. Biochem. 125, 463–470 (1982).

    Article  CAS  Google Scholar 

  19. Schmid, S. L., Matsumoto, A. K. & Rothman, J. E. Proc. natn. Acad. Sci. U.S.A. 79, 91–95 (1982).

    Article  ADS  CAS  Google Scholar 

  20. Ungewickell, E., Unanue, E. R. & Branton, D. Cold Spring Harb. Symp. quant. Biol. 46, 723–731 (1981).

    Article  CAS  Google Scholar 

  21. Brodsky, F. M., Holmes, N. J. & Parham, P. J. Cell Biol. 96, 911–914 (1983).

    Article  CAS  Google Scholar 

  22. Tack, B. F., Dean, J., Eilat, D., Lorenz, P. E. & Schechter, A. N. J. biol. Chem. 255, 8842–8847 (1980).

    CAS  PubMed  Google Scholar 

  23. Schook, W., Puszkin, S., Bloom, W., Ores, C. & Kochwa, S. Proc. natn. Acad. Sci. U.S.A. 76, 116–120 (1979).

    Article  ADS  CAS  Google Scholar 

  24. Pearse, B. M. F. Proc. natn. Acad. Sci. U.S.A. 79, 451–455 (1982).

    Article  ADS  CAS  Google Scholar 

  25. Laemmli, U. K. Nature 177, 680–685 (1970).

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Stanford University Medical School, Stanford, California, 94305, USA

    Sandra L. Schmid, William A. Braell, David M. Schlossman & James E. Rothman

Authors
  1. Sandra L. Schmid
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William A. Braell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David M. Schlossman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. James E. Rothman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schmid, S., Braell, W., Schlossman, D. et al. A role for clathrin light chains in the recognition of clathrin cages by ‘uncoating ATPase’. Nature 311, 228–231 (1984). https://doi.org/10.1038/311228a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/311228a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing