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Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse

Abstract

An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact organisms and provides a system whereby the expression and regulation of cloned genes can be followed within the context of a well characterized developmental programme.

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References

  1. Chao, M. V., Mellon, P., Charnay, P., Maniatis, T. & Axel, R. Cell 32, 483–493 (1983).

    Article  CAS  Google Scholar 

  2. Zinn, K., Mellon, P., Ptashne, M. & Maniatis, T. Proc. natn. Acad. Sci. U.S.A. 79, 4897–4901 (1982).

    Article  ADS  CAS  Google Scholar 

  3. Gillies, S. D., Morrison, S. L., Oi, V. T. & Tonegawa, S. Cell 33, 717–728 (1983).

    Article  CAS  Google Scholar 

  4. Huang, A. L., Ostrowski, M. C., Berard, D. & Hager, G. L. Cell 27, 245–255 (1981).

    Article  CAS  Google Scholar 

  5. Palmiter, R. D., Norstedt, G., Gelinas, R. E., Hammer, R. E. & Brinster, R. L. Science 222, 809–814 (1983).

    Article  ADS  CAS  Google Scholar 

  6. Cline, M. J. & Golde, D. W. Nature 277, 177–181 (1979).

    Article  ADS  CAS  Google Scholar 

  7. Nathan, D. G. & Oski, F. A. (eds) Hematology of Infancy and Childhood (Saunders, Philadelphia, 1981).

  8. Ferrero, D. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4114–4118 (1983).

    Article  ADS  CAS  Google Scholar 

  9. Johnson, G. R. J. cell. Physiol. 103, 371–383 (1980).

    Article  CAS  Google Scholar 

  10. McLeod, D. L., Shreeve, M. M. & Axelrad, A. A. Blood 44, 517–534 (1974).

    CAS  PubMed  Google Scholar 

  11. Till, J. E. & McCulloch, E. A. Radiat. Res. 14, 213–222 (1961).

    Article  ADS  CAS  Google Scholar 

  12. Lewis, J. P. & Trobaugh, F. E. Jr Nature 204, 589–590 (1964).

    Article  ADS  CAS  Google Scholar 

  13. Cline, M. J. et al. Nature 284, 422–425 (1980).

    Article  ADS  CAS  Google Scholar 

  14. Carr, F., Medina, W. D., Dube, S. & Bertino, J. R. Blood 62, 180–185 (1982).

    Google Scholar 

  15. Mulligan, R. C. Experimental Manipulation of Gene Expression (ed. Inouye, M.) 155–173 (Academic, Orlando, Florida, 1983).

    Book  Google Scholar 

  16. Cepko, C. & Mulligan, R. C. Cell (in the press).

  17. Dexter, T. M., Allen, T. D. & Lajtha, L. G. J. cell. Physiol. 91, 335–344 (1976).

    Article  Google Scholar 

  18. Mauch, P., Greenberger, J. S., Botnick, L., Hannon, E. & Hellman, S. Proc. natn. Acad. Sci. U.S.A. 77, 2927–2930 (1980).

    Article  ADS  CAS  Google Scholar 

  19. Curry, J. L. & Trentin, J. J. Devl Biol. 15, 395–413 (1967).

    Article  CAS  Google Scholar 

  20. Magli, M. C., Iscove, N. N. & Odartchenko, N. Nature 245, 527–529 (1982).

    Article  ADS  Google Scholar 

  21. Mann, R., Mulligan, R. C. & Baltimore, D. Cell 33, 153–159 (1983).

    Article  CAS  Google Scholar 

  22. Van de Woude, G. F. et al. Cold Spring Harb. Symp. quant. Biol. 44, 735–745 (1979).

    Article  Google Scholar 

  23. Nunberg, J. H., Kaufman, R. J., Chang, A. C. Y., Cohen, S. N. & Sckimke, R. J. Cell 19, 355–364 (1980).

    Article  CAS  Google Scholar 

  24. Southern, P. J. & Berg, P. J. molec. appl. Genet. 1, 327–341 (1982).

    CAS  Google Scholar 

  25. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  26. Barnes, D. W. H., Evans, E. P., Ford, C. E. & West, B. J. Nature 219, 518–520 (1968).

    Article  ADS  Google Scholar 

  27. Wu, A. M., Till, J. E., Siminovitch, L. & McCulloch, E. A. J. cell. Physiol. 69, 177–184 (1967).

    Article  CAS  Google Scholar 

  28. Weiss, R., Teich, N., Varmus, H. & Coffin, J. (eds) Molecular Biology of Tumor Virus—RNA Tumor Virus (Cold Spring Harbor Laboratory, New York, 1982).

  29. Hearings before the Subcommittee on Investigations and Oversight of the Committee on Science and Technology U.S. House of Representatives, 97th Congress, 170 (US Government Printing Office, Washington, 1983).

  30. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  Google Scholar 

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Williams, D., Lemischka, I., Nathan, D. et al. Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse. Nature 310, 476–480 (1984). https://doi.org/10.1038/310476a0

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