Abstract
A molecular recombinant of Rous sarcoma virus and murine amphotropic leukaemia virus, src(MoMuLV), where the avian src oncogene has been placed under the influence of a murine virus promoter sequence, has been reported1. Infection of long-term marrow cultures2 with this virus led to a dramatic change in the relative numbers of stem cells, granulocyte–macrophage progenitor cells and mature cells found in normal haematopoietic cell development3. However, although the balance between self-renewal, differentiation and development was disturbed, injection of the cultured cells into irradiated syngeneic recipients did not lead to the development of leukaemia. Thus, although the control had been ‘loosened’, the host regulatory mechanisms were sufficient to impose a restraint on unlimited growth of the cells. We now show that the stem cells from the src-infected cultures show a remarkably increased capacity for self-renewal in vitro in situations which are inimical to the maintenance of self-renewal in normal uninfected stem cells and that self-renewal/differentiation can be modified by the culture conditions.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Anderson, S. M. & Scolnick, E. M. J. Virol. 46, 594–605 (1983).
Dexter, T. M., Allen, T. D. & Lajtha, L. G. J. cell. Physiol. 91, 335–344 (1977).
Boettiger, D., Anderson, S. & Dexter, T. M. Cell 36, 763–773 (1984).
Schofield, R. & Lajtha, L. G. Blood Cells 9, 467–473 (1983).
Harrison, D. E. & Astle, C. M. J. exp. Med. 156, 1767–1779 (1982).
Siminovitch, L., Till, J. E. & McCulloch, E. A. J. cell. comp. Physiol. 64, 23–31 (1964).
Mauch, P., Greenberger, J. S., Botnick, L., Hannon, E. & Hellman, S. Proc. natn. Acad. Sci. U.S.A. 77, 2927–2930 (1980).
Dexter, T. M. et al. in Aplastic Anemia (Liss, New York, in the press).
Schofield, R. & Dexter, T. M. Leuk. Res. (in the press).
Dexter, T. M. & Allen, T. D. J. Path. 141, 415–433 (1983).
Metcalf, D., Johnson, G. R. & Mandel, T. E. J. cell. Physiol. 98, 401–412 (1979).
Humphries, R. K., Eaves, A. C. & Eaves, C. J. Proc. natn. Acad. Sci. U.S.A. 78, 3629–3633 (1981).
Iscove, N. N., Roitsch, C. A., Williams, N. & Guilbert, L. J. J. cell. Physiol. Suppl. 1, 65–78 (1982).
Bazill, G. W., Haynes, M., Garland, J. & Dexter, T. M. Biochem. J. 210, 747–759 (1983).
Burgess, A. W., Metcalf, D., Russell, S. H. M. & Nicola, N. A. Biochem. J. 185, 301–314 (1980).
Stanley, E. R. & Guilbert, L. J. J. immun. Meth. 42, 253–284 (1981).
Dexter, T. M., Garland, J., Scott, D., Scolnick, E. & Metcalf, D. J. exp. Med. 152, 1036–1047 (1980).
Greenberger, J. S. J. supramolec. Struct. 13, 501–511 (1980).
Schrader, J. W., Lewis, S. J., Clark-Lewis, I. & Culvenor, J. A. Proc. natn. Acad. Sci. U.S.A. 78, 323–327 (1981).
Brugge, E. & Erikson, R. L. Nature 269, 346–348 (1977).
Dexter, T. M., Scott, D. & Teich, N. M. Cell 12, 355–364 (1977).
Dexter, T. M., Allen, T. D., Testa, N. G. & Scolnick, E. J. exp. Med. 154, 594–608 (1981).
Greenberger, J. S. J. natn. Cancer Inst. 62, 337–348 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Spooncer, E., Boettiger, D. & Dexter, T. Continuous in vitro generation of multipotential stem cell clones from src-infected cultures. Nature 310, 228–230 (1984). https://doi.org/10.1038/310228a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/310228a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
