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DNA sequence and expression of the B95-8 Epstein—Barr virus genome

Nature volume 310, pages 207–211 (1984)Cite this article

Abstract

The complete (172,282 base pairs) nucleotide sequence of the B95-8 strain of Epstein–Barr virus has been established using the dideoxynucleotide/M13 sequencing procedure. Many RNA polymerase II promoters have been mapped and the mRNAs from these promoters have been assigned to the latent or early/late productive virus cycles. Likely protein-coding regions have been identified and three of these have been shown to encode a ribonucleotide reductase, a DNA polymerase and two surface glycoproteins.

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References

  1. Epstein, M. A., Achong, B. G. & Barr, Y. M. Lancet i, 702–703 (1964).

    Article  Google Scholar 

  2. Roizman, B. (ed.) The Herpesviruses, The Viruses Vol. 1 (Plenum, New York, 1982).

  3. Epstein, M. A. & Achong, B. G. (eds) The Epstein–Barr Virus (Springer, Berlin, 1979).

    Google Scholar 

  4. Henle, W., Diehl, V., Kohn, G., zur Hausen, H. & Henle, G. Science 157, 1064–1065 (1967).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Pope, J. H., Horne, M. K. & Scott, W. Int. J. Cancer 3, 857–866 (1968).

    Article  CAS  PubMed  Google Scholar 

  6. Jondal, M. & Klein, G. J. exp. Med. 138, 1365–1378 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Morgan, D. G., Niederman, J. C., Miller, G., Smith, H. W. & Dowaliby, J. M. Lancei ii, 1154–1157 (1979).

    Article  Google Scholar 

  8. Lemon, S. M., Hutt, L. M., Shaw, J. E., Li, J. L. H. & Pagano, J. S. Nature 268, 268–270 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Miller, G., Shope, T., Lisco, H., Stitt, D. & Lipman, M. Proc. natn. Acad. Sci. U.S.A. 69, 383–387 (1972).

    Article  ADS  CAS  Google Scholar 

  10. zur Hausen, H., Hecker, E., O'Neill, F. J. & Freese, U. K. Nature 272, 373–375 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Pritchett, R. F., Hayward, S. D. & Kieff, E. J. Virol. 15, 556–569 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Hayward, S. D. & Kieff, E. J Virol. 23, 421–429 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Lindahl, T. et al. J. molec. Biol. 102, 511–530 (1976).

    Article  CAS  PubMed  Google Scholar 

  14. Dambaugh, T. et al. Proc. natn. Acad. Sci. U.S.A. 77, 2999–3003 (1980).

    Article  ADS  CAS  Google Scholar 

  15. Given, D., Yee, D., Griem, K. & Kieff, E. J. Virol. 30, 852–862 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Kintner, C. R. & Sugden, B. Cell 17, 661–671 (1979).

    Article  CAS  PubMed  Google Scholar 

  17. Given, D. & Kieff, E. J. Virol. 31, 315–324 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Rymo, L. & Forsblom, S. Nucleic Acids Res. 5, 1387–1402 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Hayward, S. D., Nogee, L. & Hayward, G. S. J. Virol. 33, 507–521 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Skare, J. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 77, 3860–3864, 7510 (1980).

    Article  ADS  CAS  Google Scholar 

  21. Arrand, J. R. et al. Nucleic Acids Res. 9, 2999–3014 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Hummel, M. & Kieff, E. J. Virol. 43, 262–272 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Weigel, R. & Miller, G. Virology 125, 287–298 (1983).

    Article  CAS  PubMed  Google Scholar 

  24. Hummel, M. & Kieff, E. Proc. natn. Acad. Sci. U.S.A. 79, 5698–5702 (1982).

    Article  ADS  CAS  Google Scholar 

  25. Seibl, R. & Wolf, H. Virology (submitted).

  26. Cohen, L. K., Spech, S. H., Roberts, B. E. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. (in the press).

  27. Deininger, P. Analyt. Biochem. 129, 216–223 (1983).

    Article  CAS  PubMed  Google Scholar 

  28. Messing, J. & Vieira, J. Gene 19, 269–276 (1982).

    Article  CAS  PubMed  Google Scholar 

  29. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  30. Staden, R. Nucleic Acids Res. 10, 4731–4751 (1982); 12, 499–503 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Bankier, A. T. & Barrell, B. G. Techniques in the Life Sciences Vol. B5 (Elsevier, Ireland, 1983).

    Google Scholar 

  32. Bankier, A. T. et al. Molec. Biol. Med. 1, 425–445 (1983).

    CAS  PubMed  Google Scholar 

  33. Bankier, A. T., Deininger, P. L., Farrell, P. J. & Barrell, B. G. Molec. Biol. Med. 1, 21–45 (1983).

    CAS  PubMed  Google Scholar 

  34. Seguin, C. S., Farrell, P. J. & Barrell, B. G. Molec. Biol. Med. 1, 369–392 (1983).

    CAS  PubMed  Google Scholar 

  35. Biggin, M., Farrell, P. J. & Barrell, B. G. EMBO J. 3, 1083–1090 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Gibson, T., Stockwell, P., Ginsberg, M. & Barrell, B. G. Nucleic Acids Res. (in the press).

  37. Farrell, P. J., Deininger, P. L., Bankier, A. T. & Barrell, B. G. Proc. natn. Acad. Sci. U.S.A. 80, 1565–1569 (1983).

    Article  ADS  CAS  Google Scholar 

  38. Farrell, P. J., Bankier, A., Seguin, C., Deininger, P. & Barrell, B. G. EMBO J. 2, 1331–1338 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Jeang, K. & Hayward, S. D. J. Virol. 48, 135–148 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  40. Jones, M., Foster, L., Sheedy, T. & Griffin, B. E. EMBO J. 3, 813–821 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Hayward, S. D., Lazarowitz, S. G. & Hayward, G. S. J. Virol. 43, 201–212 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  42. Jones, M. D. & Griffin, B. E. Nucleic Acids Res. 11, 3919–3937 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Cheung, A. & Kieff, E. J. Virol. 44, 286–294 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  44. Hennessy, K. & Kieff, E. Proc. natn. Acad. Sci. U.S.A. 80, 5665–5669 (1983).

    Article  ADS  CAS  Google Scholar 

  45. Staden, R. Nucleic Acids Res. 12, 521–538 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Raab-Traub, N., Pritchett, R. & Kieff, E. J. Virol. 27, 388–398 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  47. Delius, H. & Bornkamm, G. W. J. Virol. 27, 81–89 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  48. Arrand, J. R. & Rymo, L. J. Virol. 41, 376–379 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  49. Rosa, M. D., Gottlieb, E., Lerner, M. R. & Steitz, J. A. Molec. cell. Biol. 1, 785–796 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Bhat, R. A. & Thimmappaya, B. Proc. natn. Acad. Sci. U.S.A. 80, 4789–4793 (1983).

    Article  ADS  CAS  Google Scholar 

  51. Yates, J., Warren, N., Reisman, D. & Sugden, B. Proc. natn. Acad. Sci. U.S.A. (in the press).

  52. Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. Proc. natn. Acad. Sci. U.S.A. 77, 3855–3859 (1980).

    Article  ADS  CAS  Google Scholar 

  53. Corden, J. et al. Science 209, 1406–1414 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  54. Summers, W. C. & Klein, G. J. Virol. 18, 151–155 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  55. Herz, C. & Roizman, B. Cell 33, 145–151 (1983).

    Article  CAS  PubMed  Google Scholar 

  56. Kieff, E. et al. J. infect. Dis. 146, 506–517 (1982).

    Article  CAS  PubMed  Google Scholar 

  57. Rymo, L. J. Virol. 32, 8–18 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  58. Van Santen, V., Cheung, A., Hummel, M. & Kieff, E. J. Virol. 46, 424–433 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  59. Heller, M., van Santen, V. & Kieff, E. J. Virol. 44, 311–320 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  60. Fischer, D. K. et al. Proc. natn. Acad. Sci. U.S.A. 81, 43–47 (1984).

    Article  ADS  CAS  Google Scholar 

  61. Hennessy, K., Heller, M., van Santen, V. & Kieff, E. Science 220, 1396–1398 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  62. Grogan, E. A. et al. Proc. natn. Acad. Sci. U.S.A. 80, 7650–7653 (1983).

    Article  ADS  CAS  Google Scholar 

  63. Swain, M. A. & Galloway, D. A. J. Virol. 46, 1045–1050 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  64. Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. EMBO J. 1, 945–951 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  65. Hofmann, G. J., Lazarowitz, S. G. & Hayward, S. D. Proc. natn. Acad. Sci. U.S.A. 77, 2979–2983 (1980).

    Article  ADS  Google Scholar 

  66. Thorley-Lawson, D. A. & Poodry, C. A. J. Virol. 43, 730–736 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  67. Hummel, M., Thorley-Lawson, D. A. & Kieff, E. J. Virol. 49, 413–417 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  68. Klein, G. Cell 32, 311–315 (1983).

    Article  CAS  PubMed  Google Scholar 

  69. Griffin, B. E. & Karran, L. Nature 309, 78–82 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

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Author information

Author notes

  1. R. Baer

    Present address: Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York, 10021, USA

  2. P. L. Deininger

    Present address: LSU Medical Center, 1901 Perdido St, New Orleans, Louisiana, 70112, USA

  3. C. Séguin

    Present address: Cellular Regulation Section, Laboratory of Biochemistry, NCI, National Institutes of Health, Bethesda, Maryland, 20205, USA

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 2QH, UK

    R. Baer, A. T. Bankier, M. D. Biggin, P. L. Deininger, T. J. Gibson, G. Hatfull, G. S. Hudson, S. C. Satchwell, C. Séguin & B. G. Barrell

  2. Ludwig Institute for Cancer Research, MRC Centre, Hills Road, Cambridge, CB2 2QH, UK

    P. J. Farrell & P. S. Tuffnell

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Baer, R., Bankier, A., Biggin, M. et al. DNA sequence and expression of the B95-8 Epstein—Barr virus genome. Nature 310, 207–211 (1984). https://doi.org/10.1038/310207a0

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