Control of tubulin gene expression in the parasitic protozoan Leishmania enriettii

Abstract

The life cycle of parasitic protozoa of the genus Leishmania consists of two morphologically distinct forms1: (1) amastigotes, the form of the parasite that resides inside macrophages of the mammalian host, which are non-motile and possess only a residual flagellum; and (2) promastigotes, the extracellular forms that live in the gut of the sandfly vector, which are highly motile and possess a single prominent flagellum. During the developmental transformation of amastigotes to promastigotes, the biosynthesis of α-and β-tubulin proteins is dramatically increased2, presumably to provide sufficient tubulin for synthesis and maintenance of the flagellum. We show here that the level of α-and β-tubulin mRNA in Leishmania enrietti promastigotes is significantly greater than that in amastigotes, as determined by both Northern blot analysis and by in vitro translation of cellular RNA. These results show that the regulated expression of the tubulin genes is controlled at the level of mRNA accumulation in L. enriettii, by contrast with Leishmania mexicana, in which the control of gene expression has been reported to be at the level of translation.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Zuckerman, A. & Lainson, R. in Parasitic Protozoa Vol. 1 (ed. Krier, J. P.) 57–133 (Academic, New York, 1979).

    Google Scholar 

  2. 2

    Fong, D. & Chang, K.-P. Proc. natn. Acad. Sci. U.S.A. 78, 7624–7628 (1981).

    ADS  CAS  Article  Google Scholar 

  3. 3

    Wallach, M. W., Fong, D. & Chang, K.-P. Nature 299, 650–652 (1982).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Landfear, S. M., McMahon-Pratt, D. & Wirth, D. F. Molec. Cell Biol. 3, 1070–1076 (1983).

    CAS  Article  Google Scholar 

  5. 5

    Wirth, D. F. & Slater, C. Molec. biochem. Parasit. 9, 83–92 (1983).

    CAS  Article  Google Scholar 

  6. 6

    Silflow, C. D. & Rosenbaum, J. L. Cell. 24, 81–88 (1981).

    CAS  Article  Google Scholar 

  7. 7

    Lefebvre, P. A., Silflow, C. D., Wieben, E. D. & Rosebaum, J. L. Cell. 20, 469–477 (1980).

    CAS  Article  Google Scholar 

  8. 8

    Ben-Ze'ev, A., Farmer, S. R. & Penman, S. Cell 17, 319–325 (1979).

    CAS  Article  Google Scholar 

  9. 9

    Kalfayan, L. & Wensink, P. C. Cell 29, 91–98 (1982).

    CAS  Article  Google Scholar 

  10. 10

    Raff, E. C. et al. Cell 28, 33–40 (1982).

    CAS  Article  Google Scholar 

  11. 11

    Lai, E. K., Walsh, C., Wardell, D. & Fulton, C. Cell 17, 867–878 (1979).

    CAS  Article  Google Scholar 

  12. 12

    Maniatis, T., Fritsch, E. F. & Sambrook, J. in Molecular Cloning, A Laboratory Manual, 188–196 (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  13. 13

    Pelham, H. R. B. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1976).

    CAS  Article  Google Scholar 

  14. 14

    Laemmli, U. K. Nature 227, 680–685 (1970).

    ADS  CAS  Article  Google Scholar 

  15. 15

    Rigby, P. W. J., Deckman, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    CAS  Article  Google Scholar 

  16. 16

    Unnasch, T. & Wirth, D. F. Nucleic Acids Res. 11, 8461–8472 (1983).

    CAS  Article  Google Scholar 

  17. 17

    Chang, K.-P. Science 209, 1240–1242 (1980).

    ADS  CAS  Article  Google Scholar 

  18. 18

    Fong, D. et al. Proc. natn. Acad. Sci. USA (in the press).

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Landfear, S., Wirth, D. Control of tubulin gene expression in the parasitic protozoan Leishmania enriettii. Nature 309, 716–717 (1984). https://doi.org/10.1038/309716a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.