Use of light for footprinting DNA in vivo

Abstract

A new ‘photofootprinting’ technique, which uses light to detect protein–DNA contacts as well as changes in the structure of DNA at the base pair level, has been developed and used to detect contacts between lac repressor and the lac operator in Escherichia coli cells.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Galas, D. & Schmitz, A. Nucleic Acids Res. 5, 3157–3170 (1978).

  2. 2

    Gilbert, W., Maxam, A. & Mirzabekov, A. in Control of Ribosome Synthesis (eds Kjelgaand, N. C. & Maaloe, O.) 139–148 (Munkasgaard, Copenhagen, 1976).

  3. 3

    Siebenlist, U., Simpson, R. B. & Gilbert, W. Cell 20, 269–281 (1980).

  4. 4

    Ogata, R. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 4973–4976 (1977).

  5. 5

    Ross, W., Landy, A., Kikuchi, Y. & Nash, H. Cell 18, 297–307 (1978).

  6. 6

    Van Dyke, M., Hertzberg, R. P. & Dervan, P. B. Proc. natn. Acad. Sci. U.S.A. 79, 5470–5474 (1982).

  7. 7

    Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

  8. 8

    Wang, S. Y. (ed.) Photochemistry and Photobiology of Nucleic Acids Vols 1, 2 (Academic, New York, 1976).

  9. 9

    Ciarrocchi, G. & Pedrini, A. M. J. molec. Biol. 155, 177–183 (1982).

  10. 10

    Ciarrocchi, G. & Sutherland, B. M. Photochem. Photobiol. 38, 259–263 (1983).

  11. 11

    Denhardt, D. T. & Kato, A. C. J. molec. Biol. 77, 479–494 (1973).

  12. 12

    Bose, S. N., Davies, R. J. H., Sethi, S. K. & McCloskey, J. A. Science 13, 723–725 (1983).

  13. 13

    Brown, H. C., Wheeler, O. H. & Ichikawa, K. Tetrahedron 1, 214–220 (1957).

  14. 14

    Cerutti, P. & Miller, N. J. molec. Biol. 26, 55–66 (1967).

  15. 15

    Kunieda, T. & Witkop, B. J. Am. chem. Soc. 93, 3493–3499 (1971).

  16. 16

    Miller, N. & Cerutti, P. Proc. natn. Acad. Sci. U.S.A. 59, 34–38 (1968).

  17. 17

    Green, M. & Cohen, S. S. J. biol. Chem. 225, 397–407 (1957).

  18. 18

    Roberts, T. M., Kacichi, R. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 76, 760–764 (1979).

  19. 19

    Ogata, R. T. & Gilbert, W. J. molec. Biol. 132, 709–728 (1979).

  20. 20

    Schmitz, A. & Gales, D. J. Nucleic Acids Res. 6, 111–137 (1979).

  21. 21

    Bahl, C.P., Wu, R., Stawinsky, J. & Narang, S. A. Proc. natn. Acad. Sci. U.S.A. 74, 966–970 (1977).

  22. 22

    Sancar, A. & Rupp, W. D. Cell 33, 249–260 (1983).

  23. 23

    Franklin, W. A., Lo, K. M. & Haseltine, W. A. J. biol. Chem. 257, 13535–13543 (1982).

  24. 24

    Wang, J. C. & Becherer, K. Nucleic Acids Res. 6, 1773–1790 (1983).

  25. 25

    Meistrich, M., Lamola, A. A. & Gabbay, E. Photochem. Photobiol. 11, 169–178 (1970).

  26. 26

    Turro, N. J. Molecular Photochemistry (Benjamin, Massachusetts, 1965).

  27. 27

    Gilbert, W., Majors, J. & Maxam, A. in Dahlem Workshop on Chromosomes (Abakon, Berlin, 1976).

  28. 28

    Fried, M. & Crothers, D. M. Nucleic Acids Res. 9, 6505–6526 (1981).

  29. 29

    Musajo, L. & Rodighieno, G. Photochem. Photobiol. 11, 27–35 (1970).

  30. 30

    Cole, R. S. Biochim. biophys. Acta 217, 30–39 (1970).

  31. 31

    Musajo, L., Bondin, F. & Bevilacqua, R. Photochem. Photobiol. 6, 927–931 (1967).

  32. 32

    Hansen, C. V., Shen, C.-K. J. & Hearst, J. E. Science 193, 62–64 (1976).

  33. 33

    Cech, T. R., Potter, D. & Pardue, M. L. Cold Spring Harb. Symp. quant. Biol. 42, 191–198 (1977).

  34. 34

    Chamberlain, M. J. A. Rev. Biochem. 43, 721–775 (1974).

  35. 35

    Siebenlist, U. Nature 279, 651–652 (1979).

  36. 36

    Kirkegaard, K., Buc, H., Spassky, A. & Wang, J. C. Proc. natn. Acad. Sci. U.S.A. 80, 2544–2548 (1983).

  37. 37

    Siebenlist, U., Simpson, R. B. & Gilbert, W. Cell 20, 269–281 (1980).

  38. 38

    Church, G. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 81, 1991–1995 (1984).

  39. 39

    Turchinski, M. F., Gus'kova, L. I., Khazai, I., Budovskii, E. I. & Kochetov, M. K. Molec. Biol. (USSR) 4, 343–348 (1969).

  40. 40

    Peattie, D. A. Proc. natn. Acad. Sci. U.S.A. 76, 1760–1764 (1979).

  41. 41

    Miller, J. A. Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, New York, 1972).

  42. 42

    Ish-Horowicz, D. & Burke, J. F. Nucleic Acids Res. 9, 2989–2998 (1981).

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Becker, M., Wang, J. Use of light for footprinting DNA in vivo. Nature 309, 682–687 (1984). https://doi.org/10.1038/309682a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.