Reverse gyrase—a topoisomerase which introduces positive superhelical turns into DNA

Abstract

An enzymatic activity which converts closed circular DNA into a positively supercoiled form is present in cellular extracts of Sulfolobus, an acidothermophilic archaebacterium. This novel enzyme is a type II DNA topoisomerase and is active only at temperatures > 55 °C, in the presence ofATP and Mg2+.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Gellert, M. A. Rev. Biochem. 50, 879–910 (1981).

  2. 2

    Liu, L. et al. Nature 281, 456–461 (1979).

  3. 3

    Nagata, K. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4266–4270 (1983).

  4. 4

    Kikuchi, Y. & Nash, H. Proc. natn. Acad. Sci. U.S.A. 76, 3760–3764 (1979).

  5. 5

    Bernstein, L. et al. Cell 32, 461–472 (1983).

  6. 6

    Krasnow, M. & Cozzarelli, N. Cell 32, 1313–1324 (1983).

  7. 7

    Trucksis, M. & Depew, R. Proc. natn. Acad. Sci. U.S.A. 78, 2164–2168 (1981).

  8. 8

    Sternglanz, R. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2747–2751 (1981).

  9. 9

    Crick, F. Proc. natn. Acad. Sci. U.S.A. 73, 2639–2643 (1976).

  10. 10

    Finch, J. et al. Nature 269, 29–36 (1977).

  11. 11

    Worcel, A. et al. Proc. natn. Acad. Sci. U.S.A. 78, 1461–1465 (1981).

  12. 12

    Hsieh, T-S. & Brutlag, D. Cell 21, 115–125 (1980).

  13. 13

    Baldi, M. et al. Cell 20, 461–467 (1980).

  14. 14

    Liu, L. et al. Nucleic Acids Res. 9, 3979–3989 (1981).

  15. 15

    Goto, T. & Wang, J. J. biol. Chem. 257, 5866–5872 (1982).

  16. 16

    Brown, P. & Cozzarelli, N. Science 206, 1081–1083 (1979).

  17. 17

    Gellert, M. et al. Proc. natn. Acad. Sci. U.S.A. 73, 3872–3876 (1976).

  18. 18

    Oka, A. et al. Molec. gen. Genet. 172, 151–159 (1979).

  19. 19

    Depew, R. & Wang, J. Proc. natn. Acad. Sci. U.S.A. 72, 4275–4279 (1975).

  20. 20

    Lee, C-H. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2838–2842 (1981).

  21. 21

    Liu, L. & Wang, J. Proc. natn. Acad. Sci. U.S.A. 75, 2098–2102 (1978).

  22. 22

    Brown, P. et al. Proc. natn. Acad. Sci. U.S.A. 76, 6110–6114 (1979).

  23. 23

    Snounou, G. & Malcolm, A. J. molec. Biol. 167, 211–216 (1983).

  24. 24

    Snounou, G. & Malcolm, A. Eur. J. Biochem. 138, 275–280 (1984).

  25. 25

    Yeats, S. et al. EMBO J. 1, 1035–1038 (1982).

  26. 26

    Germond, J-E. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3779–3783 (1979).

  27. 27

    Singleton, C. et al. Nature 299, 312–316 (1982).

  28. 28

    Panayotatos, N. & Wells, R. Nature 289, 466–470 (1981).

  29. 29

    Courey, A. & Wang, J. Cell 33, 817– 829 (1983).

  30. 30

    Nordheim, A. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 203, 674–679 (1983).

  31. 31

    Kohwi-Shigematsu, T. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4389–4393 (1983).

  32. 32

    Schnabel, R. et al. EMBO J. 2, 751–755 (1983).

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kikuchi, A., Asai, K. Reverse gyrase—a topoisomerase which introduces positive superhelical turns into DNA. Nature 309, 677–681 (1984). https://doi.org/10.1038/309677a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.