Abstract
An enzymatic activity which converts closed circular DNA into a positively supercoiled form is present in cellular extracts of Sulfolobus, an acidothermophilic archaebacterium. This novel enzyme is a type II DNA topoisomerase and is active only at temperatures > 55 °C, in the presence ofATP and Mg2+.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Gellert, M. A. Rev. Biochem. 50, 879–910 (1981).
Liu, L. et al. Nature 281, 456–461 (1979).
Nagata, K. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4266–4270 (1983).
Kikuchi, Y. & Nash, H. Proc. natn. Acad. Sci. U.S.A. 76, 3760–3764 (1979).
Bernstein, L. et al. Cell 32, 461–472 (1983).
Krasnow, M. & Cozzarelli, N. Cell 32, 1313–1324 (1983).
Trucksis, M. & Depew, R. Proc. natn. Acad. Sci. U.S.A. 78, 2164–2168 (1981).
Sternglanz, R. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2747–2751 (1981).
Crick, F. Proc. natn. Acad. Sci. U.S.A. 73, 2639–2643 (1976).
Finch, J. et al. Nature 269, 29–36 (1977).
Worcel, A. et al. Proc. natn. Acad. Sci. U.S.A. 78, 1461–1465 (1981).
Hsieh, T-S. & Brutlag, D. Cell 21, 115–125 (1980).
Baldi, M. et al. Cell 20, 461–467 (1980).
Liu, L. et al. Nucleic Acids Res. 9, 3979–3989 (1981).
Goto, T. & Wang, J. J. biol. Chem. 257, 5866–5872 (1982).
Brown, P. & Cozzarelli, N. Science 206, 1081–1083 (1979).
Gellert, M. et al. Proc. natn. Acad. Sci. U.S.A. 73, 3872–3876 (1976).
Oka, A. et al. Molec. gen. Genet. 172, 151–159 (1979).
Depew, R. & Wang, J. Proc. natn. Acad. Sci. U.S.A. 72, 4275–4279 (1975).
Lee, C-H. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2838–2842 (1981).
Liu, L. & Wang, J. Proc. natn. Acad. Sci. U.S.A. 75, 2098–2102 (1978).
Brown, P. et al. Proc. natn. Acad. Sci. U.S.A. 76, 6110–6114 (1979).
Snounou, G. & Malcolm, A. J. molec. Biol. 167, 211–216 (1983).
Snounou, G. & Malcolm, A. Eur. J. Biochem. 138, 275–280 (1984).
Yeats, S. et al. EMBO J. 1, 1035–1038 (1982).
Germond, J-E. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3779–3783 (1979).
Singleton, C. et al. Nature 299, 312–316 (1982).
Panayotatos, N. & Wells, R. Nature 289, 466–470 (1981).
Courey, A. & Wang, J. Cell 33, 817– 829 (1983).
Nordheim, A. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 203, 674–679 (1983).
Kohwi-Shigematsu, T. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4389–4393 (1983).
Schnabel, R. et al. EMBO J. 2, 751–755 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kikuchi, A., Asai, K. Reverse gyrase—a topoisomerase which introduces positive superhelical turns into DNA. Nature 309, 677–681 (1984). https://doi.org/10.1038/309677a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/309677a0
This article is cited by
-
Strategies of chemolithoautotrophs adapting to high temperature and extremely acidic conditions in a shallow hydrothermal ecosystem
Microbiome (2023)
-
An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research
Folia Microbiologica (2020)
-
Screening for natural inhibitors of human topoisomerases from medicinal plants with bio-affinity ultrafiltration and LC–MS
Phytochemistry Reviews (2020)
-
Increase of positive supercoiling in a hyperthermophilic archaeon after UV irradiation
Extremophiles (2019)
-
Environmental Adaptation from the Origin of Life to the Last Universal Common Ancestor
Origins of Life and Evolution of Biospheres (2018)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
