Abstract
A putative T-cell receptor gene was isolated from the DNA of the helper hybridoma, 2B4. Analysis of the sequence components of this rearranged gene indicates the presence of separate variable (V), diversity (D) and joining (J) region elements analogous to those of heavy-chain immunoglobulins. These findings further support the belief that the products of this locus are involved in antigen recognition by T cells. There is no evidence of somatic mutation between the putative germ-line and the expressed variable-region gene.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Longitudinal immunosequencing in healthy people reveals persistent T cell receptors rich in highly public receptors
BMC Immunology Open Access 21 June 2019
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Matzinger, P. Nature 292, 497–501 (1981).
Williamson, A. R. Nature 283, 527–532 (1980).
Kappler, J. W., Skidmore, B., White, J. & Marrack, P. J. exp. Med. 153, 1198–1214 (1981).
Heber-Katz, E. et al. J. exp. Med. 155, 1086–1099 (1982).
Hunig, T. R. & Bevan, M. J. J. exp. Med. 155, 111–125 (1982).
Lonai, P. et al. J. exp. Med. 154, 1910–1921 (1981).
Elliot, B. E., Nagy, Z. A., Ben-Neriah, Y. & Givol, D. Nature 285, 496–498 (1980).
Hedrick, S. M., Cohen, D. I., Nielsen, E. A. & Davis, M. M. Nature 308, 149–153 (1984).
Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I. & Davis, M. M. Nature 308, 153–158 (1984).
Yanagi, Y. et al. Nature 308, 145–149 (1984).
Samelson, L. E., Germain, R. N. & Schwartz, R. H. Proc. natn. Acad. Sci. U.S.A. 80, 6972–6976 (1983).
Allison, J. P., MacIntyre, B. W. & Block, D. J. Immun. 129, 2293–2300 (1982).
Haskins, K. et al. J. exp. Med. 157, 1149–1169 (1983).
Benoist, C. O., Mathis, D. J., Kanter, M. R., Williams, V. F. & McDevitt, H. O. Proc. natn. Acad. Sci. U.S.A. 80, 534–538 (1983).
Huynh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning Techniques: A Practical Approach (ed. Glover, D. M.) (IRL Press, Oxford, 1984).
Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. in Sequences of Proteins of Immunological Interest (US Dept of Health and Human Services, Washington, DC, 1983).
Maniatis, T. et al. Cell 15, 687–701 (1978).
Viera, J. & Messing, J. Gene 19, 259–268 (1982).
Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Parslow, T. G., Blair, D. L., Murphy, W. J. & Granner, D. K. Proc. natn. Acad. Sci. U.S.A. (in the press).
Early, P., Huang, H., Davis, M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).
Sakano, H., Maki, R., Kurosawa, Y., Roeder, W. & Tonegawa, S. Nature 286, 676–682 (1980).
Schilling, J., Clevinger, B., Davie, J. M. & Hood, L. Nature 283, 35–40 (1980).
Kurosawa, Y. & Tonegawa, S. J. exp. Med. 155, 201–218 (1982).
Sakano, H., Kurosawa, Y., Weigart, M. & Tonegawa, S. Nature 290, 562–579 (1981).
Alt, F. W. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 79, 4118–4122 (1982).
Hood, L. et al. Cold Spring Harb. Symp. quant. Biol. 45, 887–898 (1981).
Jerne, N. K. Eur. J. Immun. 1, 1–9 (1971).
Kaye, J. & Janeway, C. J. exp. Med. (submitted).
Sakano, H., Huppi, G., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).
Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).
Amzel, M., Poljak, R. J., Saul, F., Varga, J. M. & Richards, F. F. Proc. natn. Acad. Sci. U.S.A. 71, 1427–1430 (1974).
Davies, D. R., Padlan, E. A. & Segal, D. A. Rev. Biochem. 44, 629–667 (1975).
Acuto, O. et al. Cell 34, 717–726 (1983).
McIntyre, B. W. & Allison, J. P. Cell 34, 739–745 (1983).
Kappler, J., Kubo, R., Haskins, K., White, J. & Marrack, P. Cell 34, 727–737 (1983).
Marrack, P. et al. J. exp. Med. 158, 1077–1091 (1983).
Swain, S. L. Immun. Rev. 74, 129–142 (1983).
Mullins, J. I., Brody, D. S., Binari, R. C. & Cotter, S. M. Nature (in the press).
Clayberger, C., Dekruyff, R. H., Aisenberg, J. & Cantor, H. J. exp. Med. 157, 1906–1919 (1983).
Fresno, M., Nabel, G., McVay-Bordreau, L., Furthmayer, H. & Cantor, H. J. exp. Med. 153, 1246–1259 (1981).
Gascoigne, N. R. J., Chien, Y.-h., Becker, D., Kavaler, J. & Davis, M. M. Nature (submitted).
Lee, N. E. et al. J. exp. Med. (submitted).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chien, Yh., Gascoigne, N., Kavaler, J. et al. Somatic recombination in a murine T-cell receptor gene. Nature 309, 322–326 (1984). https://doi.org/10.1038/309322a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/309322a0
This article is cited by
-
Benchmarking of T cell receptor repertoire profiling methods reveals large systematic biases
Nature Biotechnology (2021)
-
Longitudinal immunosequencing in healthy people reveals persistent T cell receptors rich in highly public receptors
BMC Immunology (2019)
-
Molecules in medicine mini review: the αβ T cell receptor
Journal of Molecular Medicine (2014)
-
Immunological tolerance 50 years after the Burnet Nobel Prize
Immunology & Cell Biology (2011)
-
Transgenic mice with a diverse human T cell antigen receptor repertoire
Nature Medicine (2010)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
