Article

Cryo-electron microscopy of viruses

  • Nature volume 308, pages 3236 (01 March 1984)
  • doi:10.1038/308032a0
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Abstract

Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.

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References

  1. 1.

    Expl Cell Res. 3, 282–350 (1952).

  2. 2.

    & Science 186, 1036–1037 (1974).

  3. 3.

    & Nature 288, 569–571 (1980).

  4. 4.

    & J. Microsc. 124, RP 3–4 (1981).

  5. 5.

    , , , & J. Microsc. 128, 219–237 (1982).

  6. 6.

    , , , & Ultramicroscopy 10, 55–62 (1982).

  7. 7.

    , & J. Microsc. 129, 89–102 (1983).

  8. 8.

    , , & Proc. natn. Acad. Sci. U.S.A. 75, 4320–4324 (1978).

  9. 9.

    , & in Advances in Optical and Electron Microscopy Vol. 8 (eds Cosslett, V. E. & Barer, R.) 107–135 (Academic, London, 1982).

  10. 10.

    A. Rev. phys. Chem. 34, 593–630 (1983).

  11. 11.

    et al. J. Microsc. 132, 109–123 (1983).

  12. 12.

    et al. J. Microsc. 131, 1–9 (1983).

  13. 13.

    & Phil. Trans. R. Soc. B261 105–118 (1971).

  14. 14.

    & EMBO J. 3, 101–105 (1984).

  15. 15.

    , & J. molec. Biol. 167, 119–132 (1983).

  16. 16.

    , & J. molec. Biol. 134, 575–594 (1979).

  17. 17.

    , & J. molec. Biol. 122, 247–253 (1978).

  18. 18.

    J. molec. Biol. 90, 235–242 (1975).

  19. 19.

    , & J. gen. Virol. 45, 15–26 (1979).

  20. 20.

    & Adv. Protein Chem. 36, 79–139 (1983).

  21. 21.

    Virology 26, 567–576 (1965).

  22. 22.

    et al. J. supramolec. Struct. 2, 253–275 (1974).

  23. 23.

    & J. molec. Biol. 92, 559–565 (1975).

  24. 24.

    in The Bacteriophage T4 (eds Mathews, C. K., Kutter, E. M., Mosig, G. & Berget, P. B.) 11–24 (Am. Soc. Microbiol., Washington DC, 1983).

  25. 25.

    & J. molec. Biol. 99, 51–75 (1975).

  26. 26.

    , , & J. molec. Biol. 106, 243–275 (1976).

  27. 27.

    , & J. molec. Biol. 78, 255–259 (1973).

  28. 28.

    , , & Cell 14, 559–568 (1978).

  29. 29.

    , , , & J. molec. Biol. 71, 201–206 (1972).

  30. 30.

    , , & J. Virol. 10, 256–260 (1972).

  31. 31.

    & J. Electron Microsc. 14, 112–118 (1965).

  32. 32.

    , & Annls Med. exp. Biol. Fenn. 47, 235–248 (1969).

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Author information

Affiliations

  1. European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, FRG

    • Marc Adrian
    • , Jacques Dubochet
    • , Jean Lepault
    •  & Alasdair W. McDowall

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